Results 331 to 340 of about 657,904 (378)

Semiquantitative reverse transcription-polymerase chain reaction with the Agilent 2100 Bioanalyzer

ELECTROPHORESIS, 2001
We have applied a method to monitor mRNA expression in a semiquantitative fashion on the Agilent 2100 Bioanalyzer. The method was originally described in 1994 by Wong et al. and referred to as the "primer-dropping" method. This polymerase chain reaction (PCR) technique uses multiple sets of primer pairs in a coamplification reaction that amplifies the ...
Gottwald, E., Müller, O., Polten, A.
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Mapping Plastid Transcript Population by Circular Reverse Transcription Polymerase Chain Reaction

2018
During evolution of photosynthetic organisms, the genetic information provided by the internalized cyanobacteria has been transferred to the nucleus. The small genome kept by the chloroplast, the so-called plastome, displays a complex organization, comprising operons under the control of multiples promoters.
Courtois, Florence, Merendino Iseni, Livia   +1 more
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Reverse Transcription‐Polymerase Chain Reaction‐Based Detection of Plant Viruses

Current Protocols in Microbiology, 2007
AbstractA one‐step reverse transcription–polymerase chain reaction (RT‐PCR) is used to detect two cherry flexiviruses, Cherry green ring mottle virus (CGRMV) and Cherry necrotic rusty mottle virus (CNRMV), in Prunus species. This unit presents procedures for collection of plant samples, preparation of total nucleic acids, viral RNA‐rich or total RNA ...
John Hartung, Ruhui Li
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Reverse transcription-polymerase chain reaction: An overview of the technique and its applications

Biotechnology Advances, 1993
The polymerase chain reaction (PCR) has galvanized molecular biologists by virtue of its ability to provide them with large quantities of any desired fragment (up to 11kb) of DNA. This power combined with its flexibility has also inspired many useful applications, including new methods of DNA sequencing, cloning and mutagenesis.
Nick W. Ohan, John J. Heikkila
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Analysis of Gene Expression: Reverse Transcription-Polymerase Chain Reaction

2003
Numerous techniques have been developed to measure gene expression in tissues and cells. These include coupled reverse transcription and polymerase chain reaction amplification (RT-PCR), Northern blot (see Chapter 20 ), in situ hybridization (see Chapter 21 ), RNase protection assays, dot blots, and S1 nuclease assays.
Haddad el-B, Rousell J
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Integrated reverse transcription polymerase chain reaction systems for virus detection☆

Biosensors and Bioelectronics, 2007
The current study reports on an integrated microreverse transcription polymerase chain reaction (RT-PCR) system for molecular diagnosis of microorganisms automatically. By using antibodies-conjugated superparamagnetic beads, the developed system can detect viruses with higher sensitivity and specificity when compared with traditional biological ...
Huan-Yao Lei, Kang-Yi Lien, Gwo-Bin Lee, Wan-Chi Lee   +3 more
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A Reverse Transcription–Polymerase Chain Reaction Bioassay for Avian Vitellogenin mRNA

Toxicology and Applied Pharmacology, 2001
A bioassay based on the measurement of vitellogenin (VTG) mRNA in avian embryo hepatocyte cultures by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) was developed. To allow sequence comparison and design of suitable PCR primers, a short region of VTG cDNA was cloned and sequenced for seven species of birds. Cell cultures were
William L. Casley, Thomas W. Moon, Angela Lorenzen   +2 more
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Detection of Salmonella enteritidis by reverse transcription-polymerase chain reaction (PCR)

International Journal of Food Microbiology, 1999
A reverse transcription-polymerase chain reaction (RT-PCR) method was developed for detecting mRNA from the sefA gene of Salmonella enteritidis. Detection of target mRNA was examined from cells grown in buffered peptone water at different temperatures (37, 25 and 15 degrees C) and pH (5.5, 7.2 and 8.5).
Elizabeth A Szabo, Bernard M. Mackey
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Genotyping of calves rotavirus in China by reverse transcription polymerase chain reaction

Journal of Virological Methods, 2013
To investigate the epidemical characteristics and genotype distributions of bovine rotavirus (BRV) in China, 195 fecal samples were collected from calves with diarrhea in China. Fecal samples were detected for rotavirus A antigen using ELISA. The positive samples were screened for VP7 and VP4 by RT-PCR.
Fengling Tian, Tuanjie Che, Ayimu Guli, Zhuandi Gong, Suocheng Wei   +4 more
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Quantitative reverse transcription polymerase chain reaction of a molluscan metallothionein mRNA

Aquatic Toxicology, 2001
A quantitative assay based on competitive reverse transcription polymerase chain reaction (RT-PCR) was developed for metallothionein (MT) mRNA of the mollusc Crassostrea virginica and applied to analysis of MT mRNA of hemocytes. The assay was based on titration of a competitive external standard cRNA derived from the coding region of the oyster MT mRNA.
G. Roesijadi, Rondi A. Butler
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