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Reverse Transcription Polymerase Chain Reaction
2019The polymerase chain reaction (PCR) technique was developed to amplify, with high efficiency, double-stranded or single-stranded deoxyribonucleic acid (DNA) sequences. Amplification of individual Ribonucleic acid (RNA) molecules can also be accomplished with PCR when coupled with reverse transcription (RT) of RNA into a complementary DNA (cDNA) copy. A
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Journal of Microbiological Methods, 1999
In this work we have developed reverse transcription polymerase chain reaction (RT-PCR) methods for detecting specific mRNA from enterococci, particularly vanA and vanB genes, responsible for glycopeptide resistance in this genus. mRNA from the two genes was detected immediately after RNA extraction of a midlog phase culture, determined by growth rate ...
Privitera O +7 more
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In this work we have developed reverse transcription polymerase chain reaction (RT-PCR) methods for detecting specific mRNA from enterococci, particularly vanA and vanB genes, responsible for glycopeptide resistance in this genus. mRNA from the two genes was detected immediately after RNA extraction of a midlog phase culture, determined by growth rate ...
Privitera O +7 more
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Clinical Application of Reverse Transcription-Polymerase Chain Reaction for HIV Infection
Clinics in Laboratory Medicine, 1994This article describes the current knowledge of the various RT-PCR assays and their clinical application in HIV disease. The importance of assay precision and sample handling is emphasized. A review of quantification techniques is also presented. Finally, the relevant literature describing the application of RT-PCR for both virion RNA in plasma and ...
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Detection of canine cytokine gene expression by reverse transcription-polymerase chain reaction
Veterinary Immunology and Immunopathology, 1999Further characterization of the canine immune system will greatly benefit from the availability of tools to detect canine cytokines. Our interest concerns the study on the role of cytokines in canine visceral leishmaniasis. For this purpose, we have designed specific primers using previously published sequences for the detection of canine IL-2, IFN ...
Victor P.M.G. Rutten +7 more
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Detection of tobacco rattle virus by reverse transcription and polymerase chain reaction
Journal of Virological Methods, 1992An assay involving reverse transcription followed by polymerase chain reaction specifically detected tobacco rattle virus RNA in extracts of infected plants. It detected a wide range of serological variants and typical non-particle producing NM-type isolates.
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European Journal of Clinical Investigation, 1995
Abstract.The aim of this study was to analyse the expression of CO11A1, C011A2 and CO13A1 in 6 mm diameter punch biopsies obtained from human wounds. Total RNA was isolated from biopsies taken fromSacrococcygeal pilonidalsinus excision cavities at weekly intervals between surgery and clinical closure.
D Berry +5 more
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Abstract.The aim of this study was to analyse the expression of CO11A1, C011A2 and CO13A1 in 6 mm diameter punch biopsies obtained from human wounds. Total RNA was isolated from biopsies taken fromSacrococcygeal pilonidalsinus excision cavities at weekly intervals between surgery and clinical closure.
D Berry +5 more
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Veterinary Microbiology, 1996
We developed a quantitative reverse transcription-polymerase chain reaction (RT-PCR) procedure to estimate replication status of feline immunodeficiency virus (FIV) in peripheral blood mononuclear cells (PBMCs) of cats. Primers used for the RT-PCR were designed to detect only multiple spliced transcripts of FIV and allowed us to detect the specific ...
Takeshi Mikami, Keizo Tomonaga
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We developed a quantitative reverse transcription-polymerase chain reaction (RT-PCR) procedure to estimate replication status of feline immunodeficiency virus (FIV) in peripheral blood mononuclear cells (PBMCs) of cats. Primers used for the RT-PCR were designed to detect only multiple spliced transcripts of FIV and allowed us to detect the specific ...
Takeshi Mikami, Keizo Tomonaga
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Analytical Biochemistry, 2011
A large part of the reliability of reverse transcription quantitative polymerase chain reaction (RT-qPCR) data depends on technical variations. Such variations are mainly attributable to the reverse transcription step. Standardization is a key factor in decreasing the intersample variability.
Pascal Pugniere +4 more
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A large part of the reliability of reverse transcription quantitative polymerase chain reaction (RT-qPCR) data depends on technical variations. Such variations are mainly attributable to the reverse transcription step. Standardization is a key factor in decreasing the intersample variability.
Pascal Pugniere +4 more
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Journal of Visualized Experiments, 2019
To detect rabies virus and other member species of the genus Lyssavirus within the family Rhabdoviridae, the pan-lyssavirus nested reverse transcription polymerase chain reaction (nested RT-PCR) was developed to detect the conserved region of the nucleoprotein (N) gene of lyssaviruses.
Huancheng Guo +7 more
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To detect rabies virus and other member species of the genus Lyssavirus within the family Rhabdoviridae, the pan-lyssavirus nested reverse transcription polymerase chain reaction (nested RT-PCR) was developed to detect the conserved region of the nucleoprotein (N) gene of lyssaviruses.
Huancheng Guo +7 more
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DIAGNOSIS OF TOSPOVIRUSES BY REVERSE-TRANSCRIPTION POLYMERASE CHAIN REACTION
Acta Horticulturae, 1996R. J. Weekes +3 more
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