A rapid non-enzymatic method for the preparation of HMW DNA from blood for RFLP studies.
In genetic linkage studies using the restriction fragment length polymorphism (RFLP) technique, it is essential to process effectively large numbers of blood samples. One of the problems faced when extracting DNA by standard methods is the requirement of
D. Lahiri, J. Nurnberger
semanticscholar +1 more source
An Epidemic of Respiratory and Ocular Infections Caused by the Reemergence of a Recombinant Human Adenovirus, the Novel Type HAdV-B114 (P7H3F3). [PDF]
ABSTRACT Human adenoviruses of species B (HAdV‐B) can cause upper respiratory tract infections and conjunctivitis, but also severe lower respiratory tract infections (LRTI). Although HAdV‐associated LRTI are non‐notifiable in Germany, typing data of our Adenovirus Reference Laboratory indicated an HAdV‐B3 epidemic in 2023, with 67 samples initially ...
Ganzenmueller T+5 more
europepmc +2 more sources
Exploring the Potential and Limitations of PCR-RFLP and PCR-SSCP for SNP Detection: A Review
Polymerase chain reaction–single-strand conformation polymorphism (PCR-SSCP) and PCR–restriction fragment length polymorphism (PCR-RFLP) are two independent methods used in the post-amplification genotyping of DNA variations.
H. Hashim, M. Al-Shuhaib
semanticscholar +1 more source
Polymorphism of growth hormone gene in the artificial insemination result of Madura cattle with Limousin semen as a reference for genetic selection [PDF]
Research on genetic polymorphism of growth hormone (GH) and receptor growth hormone (rGH) has not been done in crossbred of Limousin cattle, so it is interesting to be examined. Blood samples were taken from 14 Madura calves were artificially inseminated
B. Utomo, E. Safitri
doaj +1 more source
Characterization of Lactuca spp. germplasm by protein and molecular markers - a review
The genus Lactuca L. belongs to one of the largest plant families, Asteraceae. Lactuca L. is represented by ca 100 species distributed in different geographical areas and ecological conditions.
M. Dziechciarková+3 more
doaj +1 more source
Occurrence of potential vectors of phytoplasma in pear orchards with different plantation management
During 2009-2011, regular captures of Cacopsylla pyri, C. pyrisuga and C. pyricola in four pear orchards (one conventional orchard, one integrated pest management (IPM) orchard, two organic orchards) were conducted in the Czech Republic. Polymerase chain
E. Chroboková+3 more
doaj +1 more source
Characterization of Ascaris from Ecuador and Zanzibar [PDF]
To shed light on the epidemiology of ascariasis in Ecuador and Zanzibar, 177 adult worms retrieved by chemo-expulsion from either people or pigs were collected, measured and subjected to polymerase chain reaction-restriction fragment length polymorphism (
A.M. Sparks+9 more
core +3 more sources
Restriction fragment length polymorphism (RFLP) of open reading frames 6 and 7 was applied to comparative genetic analysis of live attenuated vaccine strains (Amervac-PRRS/A3, Porcilis PRRS, Ingelvac PRRS) of porcine reproductive and respiratory syndrome
E. Kosinova, I. Psikal
doaj +1 more source
Effect of human rotavirus vaccine on severe diarrhea in African infants. [PDF]
BACKGROUND: Rotavirus is the most common cause of severe gastroenteritis among young children worldwide. Data are needed to assess the efficacy of the rotavirus vaccine in African children.
Cheuvart, Brigitte B+11 more
core +1 more source