Results 1 to 10 of about 3,781 (193)

Effect of biosurfactant sophorolipids on Rhizomucor miehei lipase fermentation by Aspergillus oryzae. [PDF]

Bioresour Bioprocess, 2021
AbstractIn this study, the effect of biosurfactant sophorolipids (SLs) on Rhizomucor miehei lipase (RML) fermentation by Aspergillus oryzae was investigated. With the exogenous addition of 0.3% (w/v) SLs in the initial medium, the RML activity reached 430.0 U/mL, an increase of 25.0% compared to the control group.
Zhang Q, Xiong Z, Sun L, Tian X, Tian G, Yang Y, Li X, Wang Y, Chu J.   +8 more
europepmc   +5 more sources

Optimization of protease production from Rhizomucor miehei Rm4 isolate under solid-state fermentation. [PDF]

J Genet Eng Biotechnol, 2022
Protease is one of the most important industrial enzymes. The importance of protease bioproduction comes from meeting the increasing demand for this enzyme especially in the cheese industry. Rhizomucor miehei protease is the preferred substitute for the traditional rennet. Solid-state fermentation (SSF) shows promising results in enzyme production.
Alahmad Aljammas H, Yazji S, Azizieh A.
europepmc   +4 more sources

Safety evaluation of the food enzyme mucorpepsin from Rhizomucor miehei strain DSM 29547. [PDF]

EFSA J, 2022
The food enzyme mucorpepsin (EC 3.4.23.23) is produced with the non-genetically modified Rhizomucor miehei strain DSM 29547 by Chr. Hansen. The food enzyme is free from viable cells of the production organism. It is intended to be used in dairy processing for cheese production.
EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (EFSA CEP Panel), Lambré C, Barat Baviera JM, Bolognesi C, Cocconcelli PS, Crebelli R, Gott DM, Grob K, Lampi E, Mengelers M, Mortensen A, Rivière G, Steffensen IL, Tlustos C, Van Loveren H, Vernis L, Zorn H, Herman L, Maia J, Kovalkovicova N, Lunardi S, Liu Y, Chesson A.   +23 more
europepmc   +4 more sources

Structure-guided modification of Rhizomucor miehei lipase for production of structured lipids. [PDF]

PLoS One, 2013
To improve the performance of yeast surface-displayed Rhizomucor miehei lipase (RML) in the production of human milk fat substitute (HMFS), we mutated amino acids in the lipase substrate-binding pocket based on protein hydrophobicity, to improve esterification activity. Five mutants: Asn87Ile, Asn87Ile/Asp91Val, His108Leu/Lys109Ile, Asp256Ile/His257Leu,
Zhang JH, Jiang YY, Lin Y, Sun YF, Zheng SP, Han SY.   +5 more
europepmc   +6 more sources

Computer-Aided Design to Improve the Thermal Stability of Rhizomucor miehei Lipase. [PDF]

Foods
Lipase, a green biocatalyst, finds extensive application in the food sector. Enhancing the thermal stability of lipase presents both challenges and opportunities within the food industry. This research employed multiple rounds of cross-screening using tools like FoldX and I-Mutant 3.0 to strategically design mutations for Rhizomucor miehei lipase (RML),
Teng R, Zhang J, Tu Z, He Q, Li Y.
europepmc   +5 more sources

Computational analysis of chain flexibility and fluctuations in Rhizomucor miehei lipase [PDF]

Protein Engineering, Design and Selection, 1999
We have performed molecular dynamics simulation of Rhizomucor miehei lipase (Rml) with explicit water molecules present. The simulation was carried out in periodic boundary conditions and conducted for 1. 2 ns in order to determine the concerted protein dynamics and to examine how well the essential motions are preserved along the trajectory.
Bywater, R. P., Peters, Günther H.J.
core   +4 more sources

Theoretical studies of Rhizomucor miehei lipase activation

"Protein Engineering, Design and Selection", 1993
Computational methods have been used to study the extensive conformational change of Rhizomucor miehei lipase upon activation. The present study considers the possible activation route, the energies involved and molecular interactions during the conformational change of the lipase in a hydrophobic environment.
M, Norin, O, Olsen, A, Svendsen, O, Edholm, K, Hult   +4 more
doaj   +8 more sources

Improved production of a recombinant Rhizomucor miehei lipase expressed in Pichia pastoris and its application for conversion of microalgae oil to biodiesel. [PDF]

Biotechnol Biofuels, 2014
Part 2: Trust and PrivacyInternational audienceTrust and privacy have been widely studied as key issues and success factors for e-commerce. The advent of m-commerce calls for revisiting these concepts and re-examining their antecedents in the mobile ...
Huang J, Xia J, Yang Z, Guan F, Cui D, Guan G, Jiang W, Li Y.   +7 more
europepmc   +4 more sources

Genome sequence and transcriptome analyses of the thermophilic zygomycete fungus Rhizomucor miehei. [PDF]

BMC Genomics, 2014
The zygomycete fungi like Rhizomucor miehei have been extensively exploited for the production of various enzymes. As a thermophilic fungus, R. miehei is capable of growing at temperatures that approach the upper limits for all eukaryotes. To date, over hundreds of fungal genomes are publicly available.
Zhou P, Zhang G, Chen S, Jiang Z, Tang Y, Henrissat B, Yan Q, Yang S, Chen CF, Zhang B, Du Z.   +10 more
europepmc   +4 more sources

Optimized hybrid nanospheres immobilizing Rhizomucor miehei lipase for chiral biotransformation [PDF]

Process Biochemistry, 2016
The authors thank the financial support from Consolider-Ingenio MULTICAT CSD2009-00050, MAT2014-52085-C2-1-P, and Severo Ochoa Excellence Program SEV-2012-0267.
Verri F, Diaz U, MACARIO, Anastasia, Corma A, GIORDANO, Girolamo   +4 more
openaire   +2 more sources