Results 21 to 30 of about 61,046 (231)

C-terminal threonines and serines play distinct roles in the desensitization of rhodopsin, a G protein-coupled receptor

eLife, 2015
Rod photoreceptors generate measurable responses to single-photon activation of individual molecules of the G protein-coupled receptor (GPCR), rhodopsin.
Anthony W Azevedo, Thuy Doan, Hormoz Moaven, Iza Sokal, Faiza Baameur, Sergey A Vishnivetskiy, Kristoff T Homan, John JG Tesmer, Vsevolod V Gurevich, Jeannie Chen, Fred Rieke   +10 more
doaj   +1 more source

Structural basis for the allosteric modulation of rhodopsin by nanobody binding to its extracellular domain

Nature Communications, 2023
Rhodopsin is a prototypical G protein-coupled receptor (GPCR) critical for vertebrate vision. Research on GPCR signaling states has been facilitated using llama-derived nanobodies (Nbs), some of which bind to the intracellular surface to allosterically ...
Arum Wu, David Salom, John D. Hong, Aleksander Tworak, Kohei Watanabe, Els Pardon, Jan Steyaert, Hideki Kandori, Kota Katayama, Philip D. Kiser, Krzysztof Palczewski   +10 more
doaj   +1 more source

The two-photon reversible reaction of the bistable jumping spider rhodopsin-1 [PDF]

, 2019
Bistable opsins are photopigments expressed in both invertebrates and vertebrates. These light-sensitive G-protein-coupled receptors undergo a reversible reaction upon illumination.
Deupi, Xavier, Ehrenberg, David, Heberle, Joachim, Koyanagi, Mitsumasa, Lesca, Elena, Schertler, Gebhard F. X., Terakita, Akihisa, Varma, Niranjan   +7 more
core   +1 more source

Rhodopsin gene expression determines rod outer segment size and rod cell resistance to a dominant-negative neurodegeneration mutant.

PLoS ONE, 2012
Two outstanding unknowns in the biology of photoreceptors are the molecular determinants of cell size, which is remarkably uniform among mammalian species, and the mechanisms of rod cell death associated with inherited neurodegenerative blinding diseases
Brandee A Price, Ivette M Sandoval, Fung Chan, Ralph Nichols, Ramon Roman-Sanchez, Theodore G Wensel, John H Wilson   +6 more
doaj   +1 more source

Regulation of intracellular cyclic GMP concentration by light and calcium in electropermeabilized rod photoreceptors. [PDF]

, 1994
This study examines the regulation of cGMP by illumination and by calcium during signal transduction in vertebrate retinal photoreceptor cells. We employed an electropermeabilized rod outer segment (EP-ROS) preparation which permits perfusion of low ...
Coccia, V J, Cote, Rick H.
core   +2 more sources

Rhodopsin 7–The unusual Rhodopsin inDrosophila

PeerJ, 2016
Rhodopsins are the major photopigments in the fruit flyDrosophila melanogaster. Drosophilaexpress six well-characterized Rhodopsins (Rh1–Rh6) with distinct absorption maxima and expression pattern. In 2000, when theDrosophilagenome was published, a novelRhodopsingene was discovered:Rhodopsin 7(Rh7).Rh7is highly conserved among theDrosophilagenus and is
Pingkalai R. Senthilan, Charlotte Helfrich-Förster   +1 more
openaire   +4 more sources

Rhodopsin expression level affects rod outer segment morphology and photoresponse kinetics. [PDF]

PLoS ONE, 2012
The retinal rod outer segment is a sensory cilium that is specialized for the conversion of light into an electrical signal. Within the cilium, up to several thousand membranous disks contain as many as a billion copies of rhodopsin for efficient photon ...
Clint L Makino, Xiao-Hong Wen, Norman A Michaud, Henry I Covington, Emmanuele DiBenedetto, Heidi E Hamm, Janis Lem, Giovanni Caruso   +7 more
doaj   +1 more source

Highly sensitive visual restoration and protection via ectopic expression of chimeric rhodopsin in mice

iScience, 2023
Summary: Photoreception requires amplification by mammalian rhodopsin through G protein activation, which requires a visual cycle. To achieve this in retinal gene therapy, we incorporated human rhodopsin cytoplasmic loops into Gloeobacter rhodopsin ...
Yusaku Katada, Kazuho Yoshida, Naho Serizawa, Deokho Lee, Kenta Kobayashi, Kazuno Negishi, Hideyuki Okano, Hideki Kandori, Kazuo Tsubota, Toshihide Kurihara   +9 more
doaj   +1 more source

Rpgrip1 is required for rod outer segment development and ciliary protein trafficking in zebrafish [PDF]

, 2017
The authors would like to thank the Royal Society of London, the National Eye Research Centre, the Visual Research Trust, Fight for Sight, the W.H. Ross Foundation, the Rosetrees Trust, and the Glasgow Children’s Hospital Charity for supporting this work.
A Eblimit, A Hameed, AF Wright, AI Hollander den, B Kennedy, BW Draper, C Insinna, C Turney, CA Murga-Zamalloa, CM Louie, CS Mellersh, D Deretic, D Mustafi, DH Hong, EM Morrow, F Liu, G Stearns, G Ying, H Patil, H Wang, HH Arts, J Kim, J Wang, J Won, JC Booij, JE Westfall, JM Corless, JP Boylan, KL Cerveny, KL Coene, L Sang, MM Humphries, MV Nachury, O Rinner, OL Moritz, P Castagnet, PD Alfinito, PH Balm, R Bachmann-Gagescu, R Roepman, R Roepman, S Gerber, SC Neuhauss, SE Brockerhoff, SR Patnaik, TA Mavlyutov, TP Dryja, V Traverso, VS Lopes, X Shu, X Shu, X Shu., Y Wada., Y Zhao, YV Makhankov   +54 more
core   +4 more sources

Isoelectric Focusing to Quantify Rhodopsin Phosphorylation in Mouse Retina

Bio-Protocol, 2019
Rhodopsin is a G-protein coupled receptor (GPCR) that mediates vision under dim light. Upon light exposure, rhodopsin is phosphorylated at multiple serine and threonine sites at its carboxyl-terminus by rhodopsin kinase (GRK1). This, in turn, reduces its
Sowmya Bekshe Lokappa, M. Cornwall, Jeannie Chen   +2 more
doaj   +1 more source