Results 21 to 30 of about 475,848 (256)

Extraction of high-quality RNA from mouse pancreatic tumors

MethodsX, 2020
Extraction of high-quality RNA from pancreatic tumors for sequencing purposes is technically challenging, as the pancreas is an organ rich in ribonucleases.
Kranthi Kumar Chougoni, Steven R. Grossman   +1 more
doaj   +1 more source

Bovine Pancreatic RNase A: An Insight into the Mechanism of Antitumor Activity In Vitro and In Vivo

Pharmaceutics, 2022
In this investigation, we extensively studied the mechanism of antitumor activity of bovine pancreatic RNase A. Using confocal microscopy, we show that after RNase A penetration into HeLa and B16 cells, a part of the enzyme remains unbound with the ...
Islam Saber Ead Mohamed, Aleksandra V. Sen’kova, Oleg V. Markov, Andrey V. Markov, Innokenty A. Savin, Marina A. Zenkova, Nadezhda L. Mironova   +6 more
doaj   +1 more source

Molecular Dynamics Simulation of Bovine Pancreatic Ribonuclease A - CpA and Transition State-like Complexes

Journal of Physical Chemistry B, 2010
The mechanisms of enzymes are intimately connected with their overall structure and dynamics in solution. Experimentally it is considerably challenging to provide detailed atomic level information about the conformational events that occur at different ...
Elena Formoso, J. M. Matxain, X. López, D. York   +3 more
semanticscholar   +1 more source

Revisiting the Formation of a Native Disulfide Bond: Consequences for Protein Regeneration and Beyond

Molecules, 2020
Oxidative protein folding involves the formation of disulfide bonds and the regeneration of native structure (N) from the fully reduced and unfolded protein (R).
Mahesh Narayan
doaj   +1 more source

Preliminary investigation of deoxyoligonucleotide binding to ribonuclease A using mass spectrometry: An attempt to develop a lab experience for undergraduates [version 2; referees: 2 approved]

F1000Research, 2018
Deoxyoligonucleotide binding to bovine pancreatic ribonuclease A (RNase A) was investigated using electrospray ionization ion-trap mass spectrometry (ESI-IT-MS). Deoxyoligonucleotides included CCCCC (dC5) and CCACC (dC2AC2).
Daniel D. Clark
doaj   +1 more source

Construction of an RNA-Binding Protein-Related Prognostic Model for Pancreatic Adenocarcinoma Based on TCGA and GTEx Databases

Frontiers in Genetics, 2021
Background: Recently, RNA-binding proteins (RBPs) were reported to interact with target mRNA to regulate gene posttranscriptional expression, and RBP-mediated RNA modification can regulate the expression and function of proto-oncogenes and tumor ...
Xin Wen, Zhiying Shao, Zhiying Shao, Shuyi Chen, Wei Wang, Yan Wang, Jinghua Jiang, Qinggong Ma, Longzhen Zhang, Longzhen Zhang, Longzhen Zhang   +10 more
doaj   +1 more source

Preliminary investigation of deoxyoligonucleotide binding to ribonuclease A using mass spectrometry: An attempt to develop a lab experience for undergraduates [version 1; referees: 2 approved]

F1000Research, 2018
Deoxyoligonucleotide binding to bovine pancreatic ribonuclease A (RNase A) was investigated using electrospray ionization ion-trap mass spectrometry (ESI-IT-MS). Deoxyoligonucleotides included CCCCC (dC5) and CCACC (dC2AC2).
Daniel D. Clark
doaj   +1 more source

Affinity Labeling of Pancreatic Ribonuclease

Journal of Biological Chemistry, 1971
Abstract Pyridoxal phosphate can be firmly bound by reduction with sodium borohydride to many enzymes in which it occurs. Such treatment has been used to label specifically parts of peptide chains in the vicinity of the bound coenzyme. Pyridoxal phosphate may form specific complexes with many proteins for which it has no known coenzyme function. Bovine
G E, Means, R E, Feeney
openaire   +2 more sources

Ligand-Induced Variations in Structural and Dynamical Properties Within an Enzyme Superfamily

Frontiers in Molecular Biosciences, 2018
Enzyme catalysis is a complex process involving several steps along the reaction coordinates, including substrate recognition and binding, chemical transformation, and product release.
Chitra Narayanan, David N. Bernard, Khushboo Bafna, Donald Gagné, Pratul K. Agarwal, Pratul K. Agarwal, Nicolas Doucet, Nicolas Doucet   +7 more
doaj   +1 more source

Construction of Highly Stable Cytotoxic Nuclear-Directed Ribonucleases

Molecules, 2018
Ribonucleases are proteins whose use is promising in anticancer therapy. We have previously constructed different human pancreatic ribonuclease variants that are selectively cytotoxic for tumor cells by introducing a nuclear localization signal into ...
David Roura Padrosa, Jessica Castro, Alejandro Romero-Casañas, Marc Ribó, Maria Vilanova, Antoni Benito   +5 more
doaj   +1 more source