Results 1 to 10 of about 49,990 (134)
Distinct Ribonuclease H Activities in Calf Thymus [PDF]
European Journal of Biochemistry, 1975 Three enzymes with ribonuclease H activity are present in calf thymus. They have been separated on the basis of chromatographic behaviour and molecular weight. They are further distinguished from one another by their ionic requirements and sensitivity to the −SH reagent N‐ethylmaleimide.
W. Büsen, Peter Hausen
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An assay of ribonuclease H, endoribonucleases, and phosphatases. [PDF]
Proceedings of the National Academy of Sciences, 1976 An assay for ribonuclease H (EC 3.1.4.34) is described which permits the estimation of the number and the type of cleavages produced by the enzyme when acting on the ribo moiety of a DNA-RNA hybrid substrate. With six different homopolymer hybrids tested, the number of enzymically induced breaks varied widely.
J G Starvianopoulos, Erwin Chargaff
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Multiple Ribonuclease H–Encoding Genes in the Caenorhabditis elegans Genome Contrasts with the Two Typical Ribonuclease H–Encoding Genes in the Human Genome [PDF]
Molecular Biology and Evolution, 2002 Database searches of the Caenorhabditis elegans and human genomic DNA sequences revealed genes encoding ribonuclease H1 (RNase H1) and RNase H2 in each genome. The human genome contains a single copy of each gene, whereas C. elegans has four genes encoding RNase H1-related proteins and one gene for RNase H2.
Arulvathani Arudchandran +5 more
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Ribonuclease H (Hybrid) in Escherichia coli
Journal of Biological Chemistry, 1973 Abstract A ribonucleolytic enzyme (RNase H) from Escherichia coli specific for RNA-DNA hybrids has been identified, partially purified, and characterized. RNase H specifically degrades the RNA strand of purified RNA-DNA hybrids but is inactive on single- or double-stranded RNA or on DNA.
Henry I. Miller +2 more
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Molecular Diversities and Functions of Ribonucleases H
Seibutsu Butsuri, 2003 Ribonuclease H specifically cleaves the RNA strand of RNA/DNA hybrids. Based on the difference in the amino acid sequences, RNases H are classified into two major families, Type 1 and Type 2 RNases H. Type 1 and Type 2 RNases H share a common three-dimensional structure despite their poor amino acid sequence similarity.
Shigenori Kanaya
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Contributions of folding cores to the thermostabilities of two ribonucleases H [PDF]
Protein Science, 2002 AbstractTo investigate the contribution of the folding cores to the thermodynamic stability of RNases H, we used rational design to create two chimeras composed of parts of a thermophilic and a mesophilic RNase H. Each chimera combines the folding core from one parent protein and the remaining parts of the other.
Srebrenka Robic +2 more
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Ribonuclease H: the enzymes in eukaryotes [PDF]
The FEBS Journal, 2009 Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. There are two main types of RNase H, and at least one of them is present in most organisms.
Susana M. Cerritelli, Robert J. Crouch
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The folding and unfolding behavior of ribonuclease H on the ribosome [PDF]
Journal of Biological Chemistry, 2020 ABSTRACTThe health of a cell depends on accurate translation and proper protein folding; misfolding can lead to aggregation and disease. The first opportunity for a protein to fold occurs during translation, when the ribosome and surrounding environment can affect the energy landscape of the nascent chain.
Jensen, Madeleine K. +4 more
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Atomistic details of the phosphodiester cleavage of ribonuclease H [PDF]
Journal of Cheminformatics, 2011 it catalyzes the hydrolysis of phospho-diester linkages of the RNA strand in the DNA:RNAhybrid duplex. RNase H activity is encoded as a part ofthe reverse transcriptase (RT) that converts a retroviralsingle strained RNA genome into double strained DNA.Due to the RNase H activity in HIV reverse transcrip-tase (HIV-RT), it represents a promising target ...
Brigitta Elsässer, Gregor Fels
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