Results 31 to 40 of about 11,347,920 (284)
Rethinking Leucine Zipper : ribonuclease activity and structural dynamics of a ubiquitous oligomerization motif [PDF]
This dissertation focuses on structural, dynamic and catalytic properties of a Leucine Zipper (LZ) motif – a family of protein oligomerization domains which belong to the structural class of coiled coil proteins.
Nikolaev, Yaroslav
core +1 more source
HIV-1 RT-Associated RNase H review
HIV-RT is an essential enzyme for HIV replication it comprises two associated functions: RNA- and DNA-dependent DNA polymerase (RDDP & DDDP) and ribonuclease H (RNase H).
Madonna M. A. Mitry +2 more
doaj +1 more source
3,7-Dihydroxytropolones Inhibit Initiation of Hepatitis B Virus Minus-Strand DNA Synthesis
Initiation of protein-primed (-) strand DNA synthesis in hepatitis B virus (HBV) requires interaction of the viral reverse transcriptase with epsilon (ε), a cis-acting regulatory signal located at the 5’ terminus of pre-genomic RNA (pgRNA), and several ...
Ellen Bak +6 more
doaj +1 more source
Purification and Properties of Ribonuclease H of Calf Thymus [PDF]
Ribonuclease H of calf thymus has been purified better than 3000-fold to yield an almost homogeneous preparation. The enzyme, which comprises about 0.03% of the total protein in the initial extract, is a slightly acidic protein (pI = 4.95) of molecular weight of about 64,000, possibly composed of subunits. The enzyme requires a metal ion for activation;
J G, Stavrianopoulos, E, Chargaff
openaire +2 more sources
Molecular Biological Characterization of the Human Foamy Virus Reverse Transcriptase and Ribonuclease H Domains [PDF]
Foamy viruses form a separate group of retroviruses encoding a pol protein with at least four domains based on comparative sequence alignments.
Flügel, Rolf M. +2 more
core +1 more source
Evolution of ribonuclease H genes in prokaryotes to avoid inheritance of redundant genes
Background A theoretical model of genetic redundancy has proposed that the fates of redundant genes depend on the degree of functional redundancy, and that functionally redundant genes will not be inherited together.
Tomita Masaru +2 more
doaj +1 more source
An assay of ribonuclease H, endoribonucleases, and phosphatases. [PDF]
An assay for ribonuclease H (EC 3.1.4.34) is described which permits the estimation of the number and the type of cleavages produced by the enzyme when acting on the ribo moiety of a DNA-RNA hybrid substrate. With six different homopolymer hybrids tested, the number of enzymically induced breaks varied widely.
J G, Starvianopoulos, E, Chargaff
openaire +3 more sources
Folding of an isolated ribonuclease H core fragment [PDF]
AbstractBased on results from both equilibrium and kinetic hydrogen exchange studies of Escherichia coli ribonuclease HI (RNase H), a fragment of RNase H (eABCD) was designed. The sequence of eABCD contains less than half of the protein's primary sequence and includes the regions that were shown to be the most protected from hydrogen exchange in all ...
A K, Chamberlain +4 more
openaire +2 more sources
DETERMINATION OF H-ALPHA,H-BETA AND H-BETA,C' COUPLING-CONSTANTS IN C-13 LABELED PROTEINS
A sensitive method to assign H(beta) protons stereospecifically as well as to determine rotamer populations about chi1 in two 3D experiments is presented.
THURING, H. +4 more
core +1 more source
α-Amylase and ribonuclease activities were determined in embryoless rye half-seeds exhibiting different viaibility after various times of storage (2, 5 and 8 years). The half-seeds were incubated for 24, 48, 72 and 96 h with and without GA3.
T. Mierzwińska
doaj +1 more source

