Results 41 to 50 of about 49,990 (134)

BLISTER-regulated vegetative growth is dependent on the protein kinase domain of ER stress modulator IRE1A in Arabidopsis thaliana [PDF]

, 2019
The unfolded protein response (UPR) is required for protein homeostasis in the endoplasmic reticulum (ER) when plants are challenged by adverse environmental conditions.
Hong, Zheng-Hui, Kleinmanns, Julia Anna, Liu, Jian-Xiang, Qing, Tao, Schubert, Daniel   +4 more
core   +1 more source

Cationic poly(amidoamine) promotes cytosolic delivery of bovine RNase A in melanoma cells, while maintaining its cellular toxicity [PDF]

, 2015
Ribonucleases are known to cleave ribonucleic acids, inducing cell death. RNase A, a member of the ribonuclease family, generally displayed poor in vitro activity. This has been attributed to factors such as low intracellular delivery.
Akinc, Almeida, Berisio, Cafaro, Chao, Cohen, Coué, Coué, De Lorenzo, Di Donato, Ding, Dubois, Ellis, Ercole, Ferruti, Foglia, Futami, Futami, Futami, Hattori, Hunter, Iversen, Ivinova, Karayianni, Larsericsdotter, Lavignac, Lavignac, Lavignac, Ledoux, Lee, Leich, Leland, Lienqueo, Liu, Lo, Mahn, Matousek, Mattison, Nicholson, Parhamifar, Parhamifar, Pattrick, Peng, Pfeil, Pouckova, Pouckova, Richardson, Romanini, Sasisanker, Saxena, Sedlak, Seyrek, Smith, Tribet, van de Weert, Vottariello, Wang, Wilson, Xia, Yeo, Zhang   +60 more
core   +1 more source

Sequence‐specific cleavage of RNA by a hybrid ribonuclease H

FEBS Letters, 1994
Site‐specific cleavage of the 22‐, 132‐ and 534‐base RNAs by the DNA/protein hybrid R Nase H were examined. The 22‐base RNA was chemically synthesized, and 132‐ and 534‐base RNAs were prepared by run‐off transcription. The hybrid enzyme cleaves these RNAs, which contain a single target sequence, primarily at the unique phosphodiester bond within the ...
Aritomo Konishi, Yasuo Komatsu, Hideo Inoue, Shigenori Kanaya, Eiko Ohtsuka, Chieko Nakai   +5 more
openaire   +3 more sources

A conformational RNA zipper promotes intron ejection during non-conventional XBP1 mRNA splicing. [PDF]

, 2015
The kinase/endonuclease IRE1 is the most conserved signal transducer of the unfolded protein response (UPR), an intracellular signaling network that monitors and regulates the protein folding capacity of the endoplasmic reticulum (ER).
Acosta-Alvear, Diego, Mendez, Aaron S, Peschek, Jirka, Walter, Peter   +3 more
core   +1 more source

RNase P Ribozymes Inhibit the Replication of Human Cytomegalovirus by Targeting Essential Viral Capsid Proteins. [PDF]

, 2015
An engineered RNase P-based ribozyme variant, which was generated using the in vitro selection procedure, was used to target the overlapping mRNA region of two proteins essential for human cytomegalovirus (HCMV) replication: capsid assembly protein (AP ...
LIU, Fenyong, Reeves, Michael, Sheng, Jingxue, Trang, Phong, Wang, Yu, Wu, Jianguo, Yang, Zhu, Ye, Jun, Zen, Ke, Zhu, Li   +9 more
core   +3 more sources

Drosophila CG3303 is an essential endoribonuclease linked to TDP-43-mediated neurodegeneration [PDF]

, 2017
Endoribonucleases participate in almost every step of eukaryotic RNA metabolism, acting either as degradative or biosynthetic enzymes. We previously identified the founding member of the Eukaryotic EndoU ribonuclease family, whose components display ...
BOZZONI, Irene, CAFFARELLI, ELISA, CAPAUTO, DAVIDE, CAPPUCCI, UGO, CASALE, ASSUNTA MARIA, DI CARLO, VALERIO, DI FRANCO, Carmela Antonia, DI MICCO, PATRIZIO, GIOIA, UBALDO, LANEVE, Pietro, Morea, Veronica, PIACENTINI, Lucia   +11 more
core   +1 more source

Ribonucleolytic resection is required for repair of strand displaced nonhomologous end-joining intermediates [PDF]

, 2013
Nonhomologous end-joining (NHEJ) pathways repair DNA double-strand breaks (DSBs) in eukaryotes and many prokaryotes, although it is not reported to operate in the third domain of life, archaea. Here, we describe a complete NHEJ complex, consisting of DNA
Bartlett, Edward, Brissett, Nigel C, Doherty, Aidan J   +2 more
core   +1 more source

A general approach to high-yield biosynthesis of chimeric RNAs bearing various types of functional small RNAs for broad applications. [PDF]

, 2015
RNA research and therapy relies primarily on synthetic RNAs. We employed recombinant RNA technology toward large-scale production of pre-miRNA agents in bacteria, but found the majority of target RNAs were not or negligibly expressed. We thus developed a
Chen, Qiu-Xia, Urayama, Shiro, Wang, Wei-Peng, Yu, Ai-Ming, Zeng, Su   +4 more
core   +2 more sources

Inhibitory effect of 2,3,5,6-tetrafluoro-4-[4-(Aryl)-1H-1,2,3-triazol-1-yl]benzenesulfonamide derivatives on HIV reverse transcriptase associated rnase H activities [PDF]

, 2016
The HIV-1 ribonuclease H (RNase H) function of the reverse transcriptase (RT) enzyme catalyzes the selective hydrolysis of the RNA strand of the RNA:DNA heteroduplex replication intermediate, and represents a suitable target for drug development.
Carcelli, Mauro, Corona, Angela, Esposito, Francesca, Grandi, Nicole, Naesens, Lieve, Pala, Nicolino, Palomba, Michele, Rogolino, Dominga, Sanna, Vanna, Sechi, Mario, Tramontano, Enzo   +10 more
core   +2 more sources

Human mitochondrial degradosome prevents harmful mitochondrial R loops and mitochondrial genome instability [PDF]

, 2018
R loops are nucleic acid structures comprising an DNA-RNA hybrid and a displaced single-stranded DNA. These structures may occur transiently during transcription, playing essential biological functions. However, persistent R loops may become pathological
Aguilera López, Andrés, Pérez de Camino Cantos, Dolores, Silva, Sonia   +2 more
core   +1 more source