Results 71 to 80 of about 11,347,920 (284)
eLife, 2018 The conformations populated during protein folding have been studied for decades; yet, their evolutionary importance remains largely unexplored. Ancestral sequence reconstruction allows access to proteins across evolutionary time, and new methods such as
Shion An Lim +2 more
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Characterization of a novel member of the family Caulimoviridae infecting Dioscorea nummularia in the Pacific, which may represent a new genus of dsDNA plant viruses. [PDF]
PLoS ONE, 2018 We have characterized the complete genome of a novel circular double-stranded DNA virus, tentatively named Dioscorea nummularia-associated virus (DNUaV), infecting Dioscorea nummularia originating from Samoa.
Amit C Sukal +4 more
doaj +1 more source
Contributions of folding cores to the thermostabilities of two ribonucleases H [PDF]
Protein Science, 2002 AbstractTo investigate the contribution of the folding cores to the thermodynamic stability of RNases H, we used rational design to create two chimeras composed of parts of a thermophilic and a mesophilic RNase H. Each chimera combines the folding core from one parent protein and the remaining parts of the other.
Srebrenka, Robic +2 more
openaire +2 more sources
FEBS Open Bio, EarlyView.Functional screening identified PcSyn14890, a cyanobacteria‐specific protein that enhances growth and stress resistance in E. coli and Synechocystis PCC6803. Although we expected it to function as a molecular chaperone, it was unable to protect against thermal aggregation of GAPDH.
Akiyo Yamada +7 more
wiley +1 more source
Sequence‐specific cleavage of RNA by a hybrid ribonuclease H
FEBS Letters, 1994 Site‐specific cleavage of the 22‐, 132‐ and 534‐base RNAs by the DNA/protein hybrid R Nase H were examined. The 22‐base RNA was chemically synthesized, and 132‐ and 534‐base RNAs were prepared by run‐off transcription. The hybrid enzyme cleaves these RNAs, which contain a single target sequence, primarily at the unique phosphodiester bond within the ...
Nakai, Chieko +5 more
openaire +2 more sources
Fusogenic RNA Nanomodules for Fusion‐Mediated and Multiplexed siRNA Delivery
Advanced Functional Materials, EarlyView.A fusogenic lipid‐layered RNA nanomodules (L‐CRAMs) enable high‐capacity and long‐lasting siRNA delivery through membrane fusion. These nanomodules carry exceptionally large siRNA payloads, avoid conventional endosomal uptake, and release multiple functional siRNAs through Dicer‐mediated processing.
Sunghyun Moon +5 more
wiley +1 more source
1RNH Ribonuclease H in P212121
, 2013 A movie of ribonuclease H ins pacegrou P212121.
Hazel Corradi
core
DETERMINATION OF H(N),H-ALPHA AND H(N), C' COUPLING-CONSTANTS IN C-13,N-15-LABELED PROTEINS
, 1994 Sensitive three-dimensional NMR experiments, based on the E.COSY principle, are presented for the measurement of the 3J(H(N),H(alpha) and 3J(H(N),C') coupling constants in uniformly C-13- and N-15-labeled proteins.
Schleucher, J +5 more
core +2 more sources
The Plant Pathology Journal, 2019 Sugarcane bacilliform viruses (SCBV), which belong to the genus Badnavirus, family Caulimoviridae, are an important DNA virus complex that infects sugarcane.
Kashif Ahmad +5 more
doaj +1 more source