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Structure and evolution of ribonuclease P RNA
Biochimie, 1991Eubacterial RNase P contains a catalytic RNA that cleaves 5' leader sequences from precursor tRNAs. We review the current understanding of RNase P RNA structure and evolution, from the perspective of phylogenetic comparative analysis.
J W, Brown, N R, Pace
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Activation of Bacterial Ribonuclease P by Macrolides
Biochemistry, 2008The effect of macrolide antibiotic spiramycin on RNase P holoenzyme and M1 RNA from Escherichia coli was investigated. Ribonuclease P (RNase P) is a ribozyme that is responsible for the maturation of 5' termini of tRNA molecules. Spiramycin revealed a dose-dependent activation on pre-tRNA cleavage by E. coli RNase P holoenzyme and M1 RNA. The K s and V
Toumpeki, Chrisavgi +4 more
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Ribonuclease P structure and function inArchaea
Molecular Biology Reports, 1996An important approach to understanding RNA-based catalytic function by ribonuclease P is the investigation of its evolutionary diversity in structure and function. Because RNase P enzymes from all organisms are thought to share common ancestry, the fundamental features of structure and biochemistry should be conserved in all of its modern forms.
J W, Brown, E S, Haas
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1988
Ribonuclease P is an enzyme that cleaves tRNA precursors to generate the 5’ termini of mature tRNA sequences. In E. coli this enzyme consists of a catalytic RNA subunit and a protein cofactor. In the absence of direct cloning of the gene for the RNA component and functional assays of the transcript of that cloned gene, it is possible to perform other ...
Sidney Altman +2 more
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Ribonuclease P is an enzyme that cleaves tRNA precursors to generate the 5’ termini of mature tRNA sequences. In E. coli this enzyme consists of a catalytic RNA subunit and a protein cofactor. In the absence of direct cloning of the gene for the RNA component and functional assays of the transcript of that cloned gene, it is possible to perform other ...
Sidney Altman +2 more
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Modulation of ribonuclease P activity by calcipotriol
European Journal of Biochemistry, 2000The effects of cholesterol, 7‐dehydrocholesterol, vitamin D3 and several synthetic vitamin D3 analogs on ribonuclease P (RNase P) were investigated using a cell‐free system from the slime mold Dictyostelium discoideum. RNase P is an ubiquitous and essential enzyme that endonucleolytically cleaves all tRNA precursors to produce the mature 5′ end.
E, Papadimou +4 more
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Recent studies of ribonuclease P.
The FASEB Journal, 1993RNase P is an essential enzyme that is required for the biosynthesis of tRNA. It is composed of RNA and protein subunits. The RNA subunit of the enzyme derived from eubacterial sources can carry out the catalytic function by itself in vitro. Current studies of RNase P focus on structure-function relationships with respect to interactions of the RNA ...
S, Altman, L, Kirsebom, S, Talbot
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Journal of Molecular Biology, 1981
A strain of Escherichia coli lacking RNAase III and containing thermolabile RNAase E and RNAase P was labeled with 32Pi at a non-permissive temperature. RNA molecules were separated by two-dimensional polyacrylamide gel electrophoresis. Most of the small RNA species were isolated and analyzed for the presence of 5′ nucleoside triphosphates.
G, Plautz, D, Apirion
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A strain of Escherichia coli lacking RNAase III and containing thermolabile RNAase E and RNAase P was labeled with 32Pi at a non-permissive temperature. RNA molecules were separated by two-dimensional polyacrylamide gel electrophoresis. Most of the small RNA species were isolated and analyzed for the presence of 5′ nucleoside triphosphates.
G, Plautz, D, Apirion
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Escherichia coli Ribonuclease P
2001Publisher Summary Ribonuclease P is an endoribonuclease responsible for the maturation of the 5′ termini of the majority of all known tRNAs in all cell types studied to date. In Escherichia coli, RNase P consists of an RNA subunit, Ml RNA, and a small basic protein, C5.
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Mitochondrial ribonuclease P activity of Trypanosoma brucei
Molecular and Biochemical Parasitology, 2001Ribonuclease P (RNase P) is an essential enzyme that cleaves the 5' leader sequences of precursor tRNAs (pre-tRNAs) to generate mature tRNAs. The RNase P-like activity from Trypanosoma brucei mitochondria (mtRNase P) was purified over 10000-fold by sequential column chromatography.
R, Salavati, A K, Panigrahi, K D, Stuart
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2009
Two gene-targeting approaches have been developed with the use of RNase P. The first approach is based on an external guide sequence (EGS), which consists of a sequence complementary to a target mRNA and, when noncovalently complexed with RNase P, renders the target RNA susceptible to degradation by the ribonuclease.
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Two gene-targeting approaches have been developed with the use of RNase P. The first approach is based on an external guide sequence (EGS), which consists of a sequence complementary to a target mRNA and, when noncovalently complexed with RNase P, renders the target RNA susceptible to degradation by the ribonuclease.
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