Results 31 to 40 of about 112,073 (318)

One-step refolding and purification of disulfide-containing proteins with a C-terminal MESNA thioester

BMC Biotechnology, 2008
Background Expression systems based on self-cleavable intein domains allow the generation of recombinant proteins with a C-terminal thioester. This uniquely reactive C-terminus can be used in native chemical ligation reactions to introduce synthetic ...
Merkx Maarten, Meijer EW, van Baal Ingrid, Bastings Maartje MC   +3 more
doaj   +1 more source

TRIM39-RPP21 Variants (∆19InsCCC) Are Not Associated with Juvenile Idiopathic Epilepsy in Egyptian Arabian Horses. [PDF]

, 2019
Juvenile idiopathic epilepsy (JIE) is an inherited disease characterized by recurrent seizures during the first year of life in Egyptian Arabian horses. Definitive diagnosis requires an electroencephalogram (EEG) performed by a veterinary specialist.
Aleman, Monica, Dahlgren, Anna R, Finno, Carrie J, Hales, Erin N, Peterson, Janel A, Rivas, Victor N   +5 more
core   +2 more sources

Characterization of proteins by means of their buffer capacity, measured with an ISFET-based coulometric sensor-actuator system [PDF]

, 1993
Proteins form the specific selector in many biochemical sensors. A change in one of the properties of such a protein has to be detected by an appropriate transducer, which completes the biochemical sensor.
Bergveld, P., Luo, J., Olthuis, W.
core   +4 more sources

Protein disulfide-isomerase interacts with a substrate protein at all stages along its folding pathway [PDF]

, 2014
In contrast to molecular chaperones that couple protein folding to ATP hydrolysis, protein disulfide-isomerase (PDI) catalyzes protein folding coupled to formation of disulfide bonds (oxidative folding).
A Jansens, A Land, A Pirneskoski, A. Katrine Wallis, Alistair G. Irvine, AR Karala, AR Karala, AY Denisov, B van den Berg, B van den Berg, B van den Berg, CB Anfinsen, Claudia A. Blindauer, CPM van Mierlo, CPM van Mierlo, CW Gruber, DP Goldenberg, E van Anken, F Hatahet, G Kozlov, G Wagner, HC Shin, HC Shin, J Riemer, JH Laity, JL Arolas, JS Weissman, JS Weissman, L Wang, LJ Byrne, Lloyd W. Ruddock, M Narayan, Mark J. Howard, Michelle L. Rowe, Narinder Sanghera, NJ Bulleid, NJ Darby, P Klappa, P Venetianer, RA Williamson, RB Freedman, RF Goldberger, Richard A. Williamson, RJ Ellis, Robert B. Freedman, S Masui, S Talluri, Salvador Ventura, TE Creighton, TE Creighton, TE Creighton, TE Creighton, VD Nguyen, VD Nguyen, WF Vranken   +54 more
core   +8 more sources

Suppressing Kaposi’s Sarcoma-Associated Herpesvirus Lytic Gene Expression and Replication by RNase P Ribozyme

Molecules, 2023
Kaposi’s sarcoma, an AIDS-defining illness, is caused by Kaposi’s sarcoma-associated herpesvirus (KSHV), an oncogenic virus. In this study, we engineered ribozymes derived from ribonuclease P (RNase P) catalytic RNA with targeting against the mRNA ...
Yujun Liu, Yuan-Chuan Chen, Bin Yan, Fenyong Liu   +3 more
doaj   +1 more source

Inferring stabilizing mutations from protein phylogenies : application to influenza hemagglutinin [PDF]

, 2009
One selection pressure shaping sequence evolution is the requirement that a protein fold with sufficient stability to perform its biological functions.
A Akasako, A Akasako, A Cao, A Martin, A Mitraki, A Rambaut, AA Pakula, AR Dinner, AR Fersht, AR Fersht, AS Yang, AS Yang, AV Gribenko, B Steipe, B Steipe, BM Broome, C Pal, C Park, CB Anfinsen, CB Do, CM Dobson, CT Saunders, D Gilis, D Perl, D Shortle, DA Cowan, DA Drummond, DA Drummond, DD Loeb, DM Taverna, DM Taverna, E Capriotti, E Hoffmann, E van Nimwegen, EPC Rocha, Eugene I. Shakhnovich, F Chiti, F Ronquist, G Parisi, GG Brownlee, H Akashi, H Li, H Schindelin, H Zhao, H Zhou, HW Hellinga, I Keller, IE Sanchez, IMP del Pino, J Felsenstein, J Felsenstein, J Felsenstein, J Felsenstein, J Kyte, JA Wells, JB Garrett, JD Bloom, JD Bloom, JD Bloom, JD Bloom, Jesse D. Bloom, JL Thorne, JM Koshi, JP Huelsenbeck, JP Huelsenbeck, JR Cochran, JR Lepock, JV Chamary, K Ishikawa, K Ishikawa, K Katayanagi, KA Bava, KA Gray, KB Zeldovich, KJ Szretter, KL Maxwell, L Giver, L Serrano, M Dai, M Haruki, M Jacob, M Lehmann, M Matrosovich, M Ueda, M Wunderlich, Matthew J. Glassman, MD Kumar, MF Sippl, MM Garcia-Mira, MM Gromiha, MP Canadillas, MS Fornasari, MW Pantoliano, N Amin, N Goldman, N Goldman, N Lartillot, N Tong, R Godoy-Ruiz, R Godoy-Ruiz, R Godoy-Ruiz, R Guerois, R Rabadan, R Sakaue, RC Edgar, RJ Ellis, S Govindarajan, S Kimura, S Kimura, S Nakajima, S Sato, SC Choi, SH White, SJ Gamblin, SS Jaswal, U Bastolla, V Parthiban, VG Dugan, VN Uversky, W Besenmatter, WS Sandberg, WSW Wong, XJ Zhang, Y Bao, YY Tseng, Z Chen   +125 more
core   +3 more sources

Rege-1 promotes C. elegans survival by modulating IIS and TOR pathways.

PLoS Genetics, 2023
Metabolic pathways are known to sense the environmental stimuli and result in physiological adjustments. The responding processes need to be tightly controlled. Here, we show that upon encountering P. aeruginosa, C.
Yi-Ting Tsai, Chen-Hsi Chang, Hsin-Yue Tsai   +2 more
doaj   +1 more source

Modular Architecture of Bacterial RNase P Ribozymes as a Structural Platform for RNA Nanostructure Design

CHIMIA, 2018
Ribonuclease P (RNase P) is a class of enzymes involved in the processing of precursor tRNAs to remove their 5'-leader sequences. Ribonuclease P enzymes are classified into two completely distinct classes, i.e.
Yuri Nozawa, Megumi Hagihara, Shigeyoshi Matsumura, Yoshiya Ikawa   +3 more
doaj   +1 more source

A Novel Method to Isolate RNase MRP Using RNA Streptavidin Aptamer Tags

Bio-Protocol, 2023
Interactions between RNA-binding proteins and RNA molecules are at the center of multiple biological processes. Therefore, accurate characterization of the composition of ribonucleoprotein complexes (RNPs) is crucial.
Violette Charteau, Merel Derksen, Ger Pruijn   +2 more
doaj   +1 more source

Synthesis and evaluation of troponoids as a new class of antibiotics [PDF]

, 2018
Novel antibiotics are urgently needed. The troponoids [tropones, tropolones, and α-hydroxytropolones (α-HT)] can have anti-bacterial activity. We synthesized or purchased 92 troponoids and evaluated their antibacterial activities against Staphylococcus ...
Adegboyega, Patrick, Cao, Feng, Donlin, Maureen J., Elagawany, Mohamed, Elgendy, Bahaa, Meyers, Marvin J., Murelli, Ryan P., Orth, Cari, Tavis, John E.   +8 more
core   +4 more sources