The contribution of the C5 protein subunit of Escherichia coli ribonuclease P to specificity for precursor tRNA is modulated by proximal 5' leader sequences. [PDF]
RNA, 2017 Recognition of RNA by RNA processing enzymes and RNA binding proteins often involves cooperation between multiple subunits. However, the interdependent contributions of RNA and protein subunits to molecular recognition by ribonucleoproteins are ...
Niland CN +3 more
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Production of ovine Pancreatic Ribonuclease and investigation of enzyme characteristics [PDF]
پژوهشهای علوم دامی ایران, 2021 Introduction Obviously, the recent decades strategy in cancer therapy, anticancer drug discovery and drug improvement is to characterize, distinguish and validate the most promising cancer-related molecular targets to which new drugs can be designed. The
mahsa Zabetian +3 more
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Mechanistic Studies Reveal Similar Catalytic Strategies for Phosphodiester Bond Hydrolysis by Protein-only and RNA-dependent Ribonuclease P. [PDF]
J Biol Chem, 2015 Background: A protein-only ribonuclease P (PRORP) has been recently discovered. Results: PRORP activity has a single ionization (pKa ∼ 8.7) important for catalysis and a cooperative dependence on Mg2+ (nH = 2). Conclusion: PRORP uses catalytic strategies
Howard MJ, Klemm BP, Fierke CA.
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A real-time fluorescence polarization activity assay to screen for inhibitors of bacterial ribonuclease P. [PDF]
Nucleic Acids Res, 2014 Ribonuclease P (RNase P) is an essential endonuclease that catalyzes the 5′ end maturation of precursor tRNA (pre-tRNA). Bacterial RNase P is an attractive potential antibacterial target because it is essential for cell survival and has a distinct ...
Liu X, Chen Y, Fierke CA.
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Proteins Rpr2 and Pop3 increase the activity and thermal stability of yeast RNase P
RNA Biology, 2023 RNA-based enzyme RNase P is a ribonucleoprotein complex responsible primarily for 5’-maturation of tRNAs. S. cerevisiae RNase P comprises a catalytic RNA component and nine proteins. The assembly and maturation of S.
Anna Perederina +2 more
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Inhibition of hepatitis B virus gene expression and replication by ribonuclease P. [PDF]
Mol Ther, 2013 Nucleic acid-based gene interfering approaches, such as those mediated by RNA interference and RNase P-associated external guide sequence (EGS), have emerged as promising antiviral strategies.
Xia C +8 more
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Frontiers in Oncology, 2022 Ribonuclease P/MRP Subunit P40 (RPP40), a component of ribonuclease P and multimeric ribonuclease P complex, was reported as one of the promoting factors for the chemoresistance of acute myeloid leukemia and a recurrence predictor of early-stage triple ...
Jianming Tang +4 more
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Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes
Molecules, 2022 Naturally occurring ribozymes with a modular architecture are promising platforms for construction of RNA nanostructures because modular redesign enables their oligomerization.
Mst. Ayesha Siddika +7 more
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Alternative substrate kinetics of Escherichia coli ribonuclease P: determination of relative rate constants by internal competition. [PDF]
J Biol Chem, 2013 Background: RNase P acts on multiple tRNA precursors, but little is known about substrate competition. Results: Alternative substrate kinetics reveal a simple competitive mechanism allowing relative rate constants to be quantified.
Yandek LE, Lin HC, Harris ME.
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The Ribonuclease P database [PDF]
Nucleic Acids Research, 1994 The Ribonuclease P Sequence database is a compilation of RNase P sequences, sequence alignments, secondary structures, three-dimensional models, and accessory information. In its initial form, the database contains information on RNase P RNA in bacteria and archaea, and RNase P protein in bacteria.
J W, Brown +3 more
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