Interaction Domains and Nuclear Targeting Signals in Subunits of the U2 Small Nuclear Ribonucleoprotein Particle-associated Splicing Factor SF3a [PDF]
Journal of Biological Chemistry, 2011 Human splicing factor SF3a is a component of the mature U2 small nuclear ribonucleoprotein particle (snRNP) and its three subunits of 60, 66, and 120 kDa are essential for splicing in vitro and in vivo. The SF3a heterotrimer forms in the cytoplasm and enters the nucleus independently of the U2 snRNP. Here, we have analyzed domains required for in vitro
Huang CJ +3 more
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Cloning and molecular characterization of Trypanosoma cruzi U2, U4, U5, and U6 small nuclear RNAs [PDF]
Memorias do Instituto Oswaldo Cruz, 2007 Small nuclear RNAs (snRNAs) are important factors in the functioning of eukaryotic cells that form several small complexes with proteins; these ribonucleoprotein particles (U snRNPs) have an essential role in the pre-mRNA processing, particularly in ...
DL Ambrósio, MTA Silva, RMB Cicarelli
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Cell Reports, 2017 Hexanucleotide repeat expansion in the C9ORF72 gene results in production of dipeptide repeat (DPR) proteins that may disrupt pre-mRNA splicing in amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) patients.
Shanye Yin +7 more
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Small nuclear ribonucleoprotein (RNP) U2 contains numerous additional proteins and has a bipartite RNP structure under splicing conditions. [PDF]
Molecular and Cellular Biology, 1993 Small nuclear (sn) ribonucleoprotein (RNP) U2 functions in the splicing of mRNA by recognizing the branch site of the unspliced pre-mRNA. When HeLa nuclear splicing extracts are centrifuged on glycerol gradients, U2 snRNPs sediment at either 12S (under high salt concentration conditions) or 17S (under low salt concentration conditions). We isolated the
S E, Behrens +4 more
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Identification, purification, and biochemical characterization of U2 small nuclear ribonucleoprotein auxiliary factor. [PDF]
Proceedings of the National Academy of Sciences, 1989 Binding of U2 small nuclear ribonucleoprotein (snRNP) to the pre-mRNA branch site is an early step in spliceosome assembly and appears to commit a pre-mRNA to the splicing pathway. We have shown previously that this ATP-dependent binding requires a non-rnRNP factor, U2 snRNP auxiliary factor (U2AF), in addition to U2 snRNP.
P D, Zamore, M R, Green
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Malleable ribonucleoprotein machine: protein intrinsic disorder in the Saccharomyces cerevisiae spliceosome [PDF]
PeerJ, 2013 Recent studies revealed that a significant fraction of any given proteome is presented by proteins that do not have unique 3D structures as a whole or in significant parts.
Maria de Lourdes Coelho Ribeiro +9 more
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Identification and Characterization of a Yeast Gene Encoding the U2 Small Nuclear Ribonucleoprotein Particle B″ Protein [PDF]
Molecular and Cellular Biology, 1996 The inessential yeast gene MUD2 encodes a protein factor that contributes to U1 small nuclear ribonucleoprotein particle (snRNP)-pre-mRNA complex (commitment complex) formation. To identify other genes that contribute to this early splicing step, we performed a synthetic lethal screen with a MUD2 deletion strain.
J, Tang, N, Abovich, M, Rosbash
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Polypeptide components of Drosophila small nuclear ribonucleoprotein particles [PDF]
, 1991 In eukaryotes splicing of pre-mRNAs is mediated by the spliceosome, a dynamic complex of small nuclear ribonucleoprotein particles (snRNPs) that associate transiently during spliceosome assembly and the splicing reaction.
FINNEGAN, D J +4 more
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Assembly of the U2 small nuclear ribonucleoprotein from Trypanosoma brucei. A mutational analysis
Journal of Biological Chemistry, 1993 trans-Splicing in trypanosomes requires the functions of U2 and U4/U6 small nuclear (sn) RNPs. We have analyzed protein binding and assembly of the Trypanosoma brucei U2 snRNP, using specific antibodies against U2 snRNP proteins and in vitro reconstitution assays of U2 deletion derivatives and human-trypanosome hybrid RNAs.
A, Günzl +3 more
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Recognition of U1 and U2 Small Nuclear RNAs Can Be Altered by a 5-Amino-Acid Segment in the U2 Small Nuclear Ribonucleoprotein Particle (snRNP) B″ Protein and through Interactions with U2 snRNP-A′ Protein [PDF]
Molecular and Cellular Biology, 1991 We have investigated the sequence elements influencing RNA recognition in two closely related small nuclear ribonucleoprotein particle (snRNP) proteins, U1 snRNP-A and U2 snRNP-B". A 5-amino-acid segment in the RNA-binding domain of the U2 snRNP-B" protein was found to confer U2 RNA recognition when substituted into the corresponding position in the U1
R C, Bentley, J D, Keene
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