BMC Genomics, 2022 Background Alternative splicing (AS) is an important channel for gene expression regulation and protein diversification, in addition to a major reason for the considerable differences in the number of genes and proteins in eukaryotes. In plants, U2 small
Cong Gao +8 more
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Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit. [PDF]
Proceedings of the National Academy of Sciences, 1992 U2 small nuclear ribonucleoprotein auxiliary factor (U2AF), an essential mammalian splicing factor, is composed of two subunits: a 65-kDa protein (U2AF65), which binds the pre-mRNA polypyrimidine tract and is required for in vitro splicing, and an associated 35-kDa protein (U2AF35). Here we report the isolation of a cDNA encoding U2AF35.
Zhang, M. +4 more
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Mutations in an Essential U2 Small Nuclear RNA Structure Cause Cold-Sensitive U2 Small Nuclear Ribonucleoprotein Function by Favoring Competing Alternative U2 RNA Structures [PDF]
Molecular and Cellular Biology, 1994 Mutations in stem-loop IIa of yeast U2 RNA cause cold-sensitive growth and cold-sensitive U2 small nuclear ribonucleoprotein function in vitro. Cold-sensitive U2 small nuclear RNA adopts an alternative conformation that occludes the loop and disrupts the stem but does so at both restrictive and permissive temperatures.
M I, Zavanelli +3 more
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Autoantibodies to ribonucleoprotein particles containing U2 small nuclear RNA. [PDF]
The EMBO Journal, 1985 Autoantibodies exclusively precipitating U1 and U2 small nuclear ribonucleoprotein (snRNP) particles [anti-(U1,U2)RNP] were detected in sera from four patients with autoimmune disorders. When tested by immunoblotting, these sera recognized up to four different protein antigens in purified mixtures of U1-U6 RNP particles.
W, Habets +4 more
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Human U1 and U2 small nuclear ribonucleoproteins contain common and unique polypeptides. [PDF]
Molecular and Cellular Biology, 1982 Immunoprecipitation of human small nuclear ribonucleoproteins (snRNPs) containing the small nuclear RNAs U1, U2, U4, U5, and U6 with two antibodies produced in certain patients suffering from systemic lupus erythematosus was used to identify the polypeptides present on human U1 and U2 snRNPs. U1 and U2 snRNPs contain both common and unique polypeptides;
C S, Kinlaw +2 more
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The C-group Heterogeneous Nuclear Ribonucleoprotein Proteins Bind to the 5′ Stem-Loop of the U2 Small Nuclear Ribonucleoprotein Particle [PDF]
Journal of Biological Chemistry, 1996 The C-group heterogeneous nuclear ribonucleoprotein (hnRNP) proteins bind to nascent pre-messenger RNA. In vitro studies have indicated that the C hnRNP proteins bind particularly strongly to the intron polypyrimidine tract of pre-mRNA and may be important for pre-mRNA splicing.
J, Temsamani, T, Pederson
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Altered mRNA Splicing, Chondrocyte Gene Expression and Abnormal Skeletal Development due to SF3B4 Mutations in Rodriguez Acrofacial Dysostosis. [PDF]
PLoS Genetics, 2016 The acrofacial dysostoses (AFD) are a genetically heterogeneous group of inherited disorders with craniofacial and limb abnormalities. Rodriguez syndrome is a severe, usually perinatal lethal AFD, characterized by severe retrognathia, oligodactyly and ...
Felipe Marques +8 more
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Downregulation of SF3B2 protects CNS neurons in models of multiple sclerosis
Annals of Clinical and Translational Neurology, 2023 Objective Neurodegeneration induced by inflammatory stress in multiple sclerosis (MS) leads to long‐term neurological disabilities that are not amenable to current immunomodulatory therapies.
Ye Eun Jeong +4 more
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Exomeres and supermeres: Monolithic or diverse?
Journal of Extracellular Biology, 2022 Extracellular vesicles (EVs), including exosomes and microvesicles, are far from being the only RNA‐containing extracellular particles (EPs). Recently, new 35‐nm‐sized EPs were discovered by asymmetric‐flow field‐flow fractionation and termed ‘exomeres’.
Juan Pablo Tosar +2 more
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Probing Interactions between the U2 Small Nuclear Ribonucleoprotein and the DEAD-box Protein, Prp5 [PDF]
Journal of Biological Chemistry, 2002 Pre-mRNA binding to the yeast U2 small nuclear ribonucleoprotein (snRNP) during prespliceosome formation requires ATP hydrolysis, the highly conserved UACUAAC box of the branch point region of the pre-mRNA, and several factors. Here we analyzed the binding of a radiolabeled 2'-O-methyl oligonucleotide complementary to U2 small nuclear RNA to study ...
Barham K Abu, Abu Dayyeh +3 more
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