A role for the Cajal-body-associated SUMO isopeptidase USPL1 in snRNA transcription mediated by RNA polymerase II [PDF]
, 2014 Cajal bodies are nuclear structures that are involved in biogenesis of snRNPs and snoRNPs, maintenance of telomeres and processing of histone mRNA. Recently, the SUMO isopeptidase USPL1 was identified as a component of Cajal bodies that is essential for ...
Winter, Ulrike +4 more
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The U1 RNA-binding site of the U1 small nuclear ribonucleoprotein (snRNP)-associated A protein suggests a similarity with U2 snRNPs. [PDF]
Molecular and Cellular Biology, 1989 The site of interaction between human U1 RNA and one of its uniquely associated proteins, A, was examined with in vitro binding assays. The A protein bound directly to stem-loop II of U1 RNA in a region which exhibits sequence similarity to U2 RNA.
C, Lutz-Freyermuth +2 more
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Crystal Structure of Prp5p Reveals Interdomain Interactions that Impact Spliceosome Assembly
Cell Reports, 2013 The DEAD-box adenosine triphosphatase (ATPase) Prp5p facilitates U2 small nuclear ribonucleoprotein particle (snRNP) binding to the intron branch site region during spliceosome assembly. We present crystal structures of S.
Zhi-Min Zhang +7 more
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Evidence for a nuclear compartment of transcription and splicing located at chromosome domain boundaries [PDF]
, 1993 The nuclear topography of splicing snRNPs, mRNA transcripts and chromosome domains in various mammalian cell types are described. The visualization of splicing snRNPs, defined by the Sm antigen, and coiled bodies, revealed distinctly different ...
R. M. Zirbel +9 more
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Alternative Spliceosome Assembly Pathways Revealed by Single-Molecule Fluorescence Microscopy
Cell Reports, 2013 Removal of introns from nascent transcripts (pre-mRNAs) by the spliceosome is an essential step in eukaryotic gene expression. Previous studies have suggested that the earliest steps in spliceosome assembly in yeast are highly ordered and the stable ...
Inna Shcherbakova +7 more
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Evidence for base-pairing between mammalian U2 and U6 small nuclear ribonucleoprotein particles. [PDF]
Genes & Development, 1990 Intramolecular and intermolecular snRNA cross-links were generated by irradiating HeLa nuclear extract with 365 nm light in the presence of the psoralen derivative AMT. After deproteinization, cross-linked RNAs were resolved by gel electrophoresis and identified as anomalously migrating species by Northern blotting. In addition to the U4/U6 snRNA cross-
T P, Hausner, L M, Giglio, A M, Weiner
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In Vitro Studies of the Prp9·Prp11·Prp21 Complex Indicate a Pathway for U2 Small Nuclear Ribonucleoprotein Activation [PDF]
Journal of Biological Chemistry, 1996 Pre-mRNA splicing takes place on a large ribonucleoprotein particle, the spliceosome which contains the five small nuclear ribonucleoproteins (snRNPs), U1, U2, U4, U5, and U6. In Saccharomyces cerevisiae the mRNA splicing factors, Prp9, Prp11, and Prp21, are necessary for addition of the U2 snRNP to the pre-mRNA in an early step of spliceosome assembly.
D K, Wiest, C L, O'Day, J, Abelson
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Protein quality control meets transcriptome remodeling under stress
Cell Stress, 2017 To tolerate and recover from genotoxic stress cells must coordinate a range of stress response activities including cell cycle arrest, DNA repair, and remodeling of the transcriptome and proteome.
Veena Mathew, Peter C. Stirling
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Special Sm Core Complex Functions in Assembly of the U2 Small Nuclear Ribonucleoprotein of Trypanosoma brucei [PDF]
Eukaryotic Cell, 2009 ABSTRACT The processing of polycistronic pre-mRNAs in trypanosomes requires the spliceosomal small ribonucleoprotein complexes (snRNPs) U1, U2, U4/U6, U5, and SL, each of which contains a core of seven Sm proteins. Recently we reported the first evidence for a core variation in spliceosomal snRNPs; specifically, in the ...
Christian, Preusser +2 more
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Characterization of factors involved in the coupling of 3' end processing and splicing and in the 3' end formation of mRNA precursors [PDF]
, 2006 Eukaryotic mRNA precursors are processed at their 5’ and 3’ ends and are spliced prior to their export from the nucleus to the cytoplasm. Although all three processing reactions can be studied separately in vitro, they are coupled in vivo.
Kyburz Kooznetsoff, Andrea Martina
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