Molecular principles underlying dual RNA specificity in the Drosophila SNF protein [PDF]
, 2018 The first RNA recognition motif of the Drosophila SNF protein is an example of an RNA binding protein with multi-specificity. It binds different RNA hairpin loops in spliceosomal U1 or U2 small nuclear RNAs, and only in the latter case requires the ...
DeKoster, Gregory T +4 more
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Multiple Forms of the U2 Small Nuclear Ribonucleoprotein Auxiliary Factor U2AF Subunits Expressed in Higher Plants [PDF]
Journal of Biological Chemistry, 1998 Requirements for intron recognition during pre-mRNA splicing in plants differ from those in vertebrates and yeast. Plant introns contain neither conserved branch points nor distinct 3' splice site-proximal polypyrimidine tracts characteristic of the yeast and vertebrate introns, respectively. However, they are strongly enriched in U residues throughout
C, Domon +3 more
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Protein quality control meets transcriptome remodeling under stress
Cell Stress, 2017 To tolerate and recover from genotoxic stress cells must coordinate a range of stress response activities including cell cycle arrest, DNA repair, and remodeling of the transcriptome and proteome.
Veena Mathew, Peter C. Stirling
doaj +1 more source
Recognition of U1 and U2 Small Nuclear RNAs Can Be Altered by a 5-Amino-Acid Segment in the U2 Small Nuclear Ribonucleoprotein Particle (snRNP) B″ Protein and through Interactions with U2 snRNP-A′ Protein [PDF]
Molecular and Cellular Biology, 1991 We have investigated the sequence elements influencing RNA recognition in two closely related small nuclear ribonucleoprotein particle (snRNP) proteins, U1 snRNP-A and U2 snRNP-B". A 5-amino-acid segment in the RNA-binding domain of the U2 snRNP-B" protein was found to confer U2 RNA recognition when substituted into the corresponding position in the U1
R C, Bentley, J D, Keene
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Cloning and molecular characterization of Trypanosoma cruzi U2, U4, U5, and U6 small nuclear RNAs
Memorias do Instituto Oswaldo Cruz, 2007 Small nuclear RNAs (snRNAs) are important factors in the functioning of eukaryotic cells that form several small complexes with proteins; these ribonucleoprotein particles (U snRNPs) have an essential role in the pre-mRNA processing, particularly in ...
DL Ambrósio, MTA Silva, RMB Cicarelli
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αCP binding to a cytosine-rich subset of polypyrimidine tracts drives a novel pathway of cassette exon splicing in the mammalian transcriptome. [PDF]
, 2016 Alternative splicing (AS) is a robust generator of mammalian transcriptome complexity. Splice site specification is controlled by interactions of cis-acting determinants on a transcript with specific RNA binding proteins.
Bahrami-Samani, Emad +7 more
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Special Sm Core Complex Functions in Assembly of the U2 Small Nuclear Ribonucleoprotein of Trypanosoma brucei [PDF]
Eukaryotic Cell, 2009 ABSTRACT The processing of polycistronic pre-mRNAs in trypanosomes requires the spliceosomal small ribonucleoprotein complexes (snRNPs) U1, U2, U4/U6, U5, and SL, each of which contains a core of seven Sm proteins. Recently we reported the first evidence for a core variation in spliceosomal snRNPs; specifically, in the ...
Christian, Preusser +2 more
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Mud2 functions in transcription by recruiting the Prp19 and TREX complexes to transcribed genes [PDF]
, 2007 The different steps of gene expression are intimately linked to coordinate and regulate this complex process. During transcription, numerous RNA-binding proteins are already loaded onto the nascent mRNA and package the mRNA into a messenger ...
Andersen, Ulrik L. +8 more
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PLoS ONE, 2020 Mutations in the splicing machinery have been implicated in a number of human diseases. Most notably, the U2 small nuclear ribonucleoprotein (snRNP) component SF3b1 has been found to be frequently mutated in blood cancers such as myelodysplastic ...
Harpreet Kaur +4 more
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Interactome analysis brings splicing into focus [PDF]
, 2015 The spliceosome is a huge molecular machine that assembles dynamically onto its pre-mRNA substrates. A new study based on interactome analysis provides clues about how splicing-regulatory proteins modulate assembly of the spliceosome to either activate ...
Burge, Christopher B., Dominguez, Daniel
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