Results 11 to 20 of about 167,361 (378)
CRISPR prime editing with ribonucleoprotein complexes in zebrafish and primary human cells
Nature Biotechnology, 2021 Prime Editors have been delivered using DNA or mRNA vectors. Here we demonstrate prime editing (PE) with purified ribonucleoprotein (RNP) complexes. We introduced somatic mutations in zebrafish embryos with frequencies as high as 30% and demonstrate ...
Karl Petri +13 more
semanticscholar +2 more sources
Structural studies of protein–nucleic acid complexes: A brief overview of the selected techniques
Computational and Structural Biotechnology Journal, 2023 Protein–nucleic acid complexes are involved in all vital processes, including replication, transcription, translation, regulation of gene expression and cell metabolism.
Kamil Szpotkowski +2 more
doaj +1 more source
Strategies in the delivery of Cas9 ribonucleoprotein for CRISPR/Cas9 genome editing
Theranostics, 2021 CRISPR/Cas9 genome editing has gained rapidly increasing attentions in recent years, however, the translation of this biotechnology into therapy has been hindered by efficient delivery of CRISPR/Cas9 materials into target cells. Direct delivery of CRISPR/
Song Zhang +3 more
semanticscholar +1 more source
Approaches to Identify and Characterise the Post-Transcriptional Roles of lncRNAs in Cancer
Non-Coding RNA, 2021 It is becoming increasingly evident that the non-coding genome and transcriptome exert great influence over their coding counterparts through complex molecular interactions.
Jean-Michel Carter +4 more
doaj +1 more source
Science Advances, 2022 CRISPR-Cas9 gene editing has emerged as a powerful therapeutic technology, but the lack of safe and efficient in vivo delivery systems, especially for tissue-specific vectors, limits its broad clinical applications.
Tao Wan +5 more
semanticscholar +1 more source
Viruses, 2021 An evolutionary arms race has been ongoing between retroviruses and their primate hosts for millions of years. Within the last century, a zoonotic transmission introduced the Human Immunodeficiency Virus (HIV-1), a retrovirus, to the human population ...
Kevin M. Rose +6 more
doaj +1 more source
Membrane surfaces regulate assembly of ribonucleoprotein condensates
Nature Cell Biology, 2022 Biomolecular condensates organize biochemistry, yet little is known about how cells control the position and scale of these structures. In cells, condensates often appear as relatively small assemblies that do not coarsen into a single droplet despite ...
W. T. Snead +5 more
semanticscholar +1 more source
Frontiers in Genome Editing, 2022 Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) mediated genome editing is a powerful approach for crop improvement. Traditional transformation methods based on plasmid delivery pose concerns associated with transgene integration and ...
Yingxiao Zhang +11 more
doaj +1 more source
Unknown Areas of Activity of Human Ribonuclease Dicer: A Putative Deoxyribonuclease Activity
Molecules, 2020 The Dicer ribonuclease plays a crucial role in the biogenesis of small regulatory RNAs (srRNAs) by processing long double-stranded RNAs and single-stranded hairpin RNA precursors into small interfering RNAs (siRNAs) and microRNAs (miRNAs), respectively ...
Marta Wojnicka +2 more
doaj +1 more source
Frontiers in Genome Editing, 2022 Delivery of genome editing reagents using CRISPR-Cas ribonucleoproteins (RNPs) transfection offers several advantages over plasmid DNA-based delivery methods, including reduced off-target editing effects, mitigation of random integration of non-native ...
Raviraj Banakar +15 more
doaj +1 more source