The World of Stable Ribonucleoproteins and Its Mapping With Grad-Seq and Related Approaches
Frontiers in Molecular Biosciences, 2021 Macromolecular complexes of proteins and RNAs are essential building blocks of cells. These stable supramolecular particles can be viewed as minimal biochemical units whose structural organization, i.e., the way the RNA and the protein interact with each
Milan Gerovac +4 more
doaj +2 more sources
Efficient One-Step Knockout by Electroporation of Ribonucleoproteins Into Zona-Intact Bovine Embryos
Frontiers in Genetics, 2020 Somatic cell nuclear transfer or cytoplasm microinjection have been used to generate genome-edited farm animals; however, these methods have several drawbacks that reduce their efficiency. This study aimed to develop electroporation conditions that allow
Luiz Sergio Almeida Camargo +3 more
doaj +2 more sources
Nature Communications, 2019 A current challenge in genome editing is delivering Cas9 and sgRNA into target cells. Here the authors engineer a delivery system based on murine leukemia virus-like particles loaded with Cas9-sgRNA ribonucleoproteins to induce efficient genome editing ...
Philippe E. Mangeot +18 more
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Membrane permeabilizing amphiphilic peptide delivers recombinant transcription factor and CRISPR-Cas9/Cpf1 ribonucleoproteins in hard-to-modify cells. [PDF]
PLoS ONE, 2018 Delivery of recombinant proteins to therapeutic cells is limited by a lack of efficient methods. This hinders the use of transcription factors or Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) ribonucleoproteins to develop cell ...
Thomas Del'Guidice +12 more
doaj +2 more sources
Nature Communications, 2020 Therapeutic targets of CRISPR-Cas can often not be accessed due to lack of carriers to deliver RNPs systematically. Here, the authors engineer modified lipid nanoparticles for delivery of gene editing proteins to specific tissues.
Tuo Wei +4 more
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Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites
Nature Communications, 2019 Influenza A virus forms cytosolic inclusions containing viral ribonucleoproteins. Here, the authors show that viral inclusions form juxtaposed the endoplasmic reticulum and have liquid properties, likely constituting sites of assembly of epidemic and ...
Marta Alenquer +5 more
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Engineering self-deliverable ribonucleoproteins for genome editing in the brain
Nature CommunicationsThe delivery of CRISPR ribonucleoproteins (RNPs) for genome editing in vitro and in vivo has important advantages over other delivery methods, including reduced off-target and immunogenic effects.
Kai Chen +6 more
doaj +2 more sources
hnRNP A1, hnRNP A2B1, and hnRNP K are dysregulated in tauopathies, but do not colocalize with tau pathology [PDF]
Brain Pathology, Volume 35, Issue 3, May 2025.Diversity of hnRNP A1, hnRNP A2B1, and hnRNP K immunostaining across hippocampal subregions in Alzheimer's disease. Abstract Tau interacts with multiple heterogeneous nuclear ribonucleoproteins (hnRNPs)—a family of RNA binding proteins that regulate multiple known cellular functions, including mRNA splicing, mRNA transport, and translation regulation ...
Tomas Kavanagh +6 more
wiley +2 more sources
Protocol for the electroporation of CRISPR-Cas for DNA and RNA targeting in Bos taurus zygotes [PDF]
STAR ProtocolsSummary: The use of CRISPR-Cas9 ribonucleoproteins has revolutionized manipulation of genomes. Here, we present a protocol for the electroporation of CRISPR-Cas for DNA and RNA targeting in Bos taurus zygotes.
Fernando H. Biase, Gustavo Schettini
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Efficient targeted DNA editing and replacement in Chlamydomonas reinhardtii using Cpf1 ribonucleoproteins and single-stranded DNA [PDF]
Proceedings of the National Academy of Sciences of the United States of America, 2017 Ferenczi, Aron +3 more
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