Results 31 to 40 of about 24,238 (294)
Loss of function mutations in GEMIN5 cause a neurodevelopmental disorder
Nature Communications, 2021 GEMIN5, an RNA-binding protein, is required for formation of small nuclear ribonucleoproteins. Here, the authors identify loss of function mutations in GEMIN5 that are associated with a human neurodevelopmental disorder.
Sukhleen Kour +69 more
doaj +1 more source
Frontiers in Plant Science, 2018 The CRISPR/Cas9 genome editing system has already proved its efficiency, versatility and simplicity in numerous applications in human, animal, microbe and plant cells.
Jana Murovec +4 more
doaj +1 more source
Proteomic and 3D structure analyses highlight the C/D box snoRNP assembly mechanism and its control [PDF]
, 2014 In vitro, assembly of box C/D small nucleolar ribonucleoproteins (snoRNPs) involves the sequential recruitment of core proteins to snoRNAs. In vivo, however, assembly factors are required (NUFIP, BCD1, and the HSP90–R2TP complex), and it is unknown ...
Franck Vandermoere +32 more
core +1 more source
Nature Communications, 2021 Biogenesis of small nucleolar RNAs ribonucleoproteins (snoRNPs) requires dedicated assembly machinery. Here, the authors show that a subset of snoRNP assembly factors interacts, genetically or directly, with factors modulating chromatin architecture ...
Benoît Bragantini +19 more
doaj +1 more source
Preparation of Cas9 Ribonucleoproteins for Genome Editing
Bio-Protocol, 2022 Genome editing by the delivery of pre-assembled Cas9 ribonucleoproteins (Cas9 RNP) is an increasingly popular approach for cell types that are difficult to manipulate genetically by the conventional plasmid and viral methods.
SW Lin, Viet Nguyen, Steven Lin
doaj +1 more source
Ribonucleoprotein Immunoprecipitation (RIP) Analysis [PDF]
BIO-PROTOCOL, 2020 RNAs and RNA-binding proteins (RBPs) can interact dynamically in ribonucleoprotein (RNP) complexes that play important roles in controlling gene expression programs. One of the powerful ways to investigate changes in the association of RNAs with an RBP of interest is by immunoprecipitation (IP) analysis of native RNPs.
Martindale, Jennifer +2 more
openaire +3 more sources
Changes in intranuclear mobility of mature snRNPs provide a mechanism for splicing defects in spinal muscular atrophy [PDF]
, 2012 This work was funded by the Wellcome Trust (grant ID WT078810MA)It is becoming increasingly clear that defects in RNA metabolism can lead to disease. Spinal muscular atrophy (SMA), a leading genetic cause of infant mortality, results from insufficient ...
Clelland, Allyson Kara +2 more
core +1 more source
Comparative analysis of viral RNA signatures on different RIG-I-like receptors
eLife, 2016 The RIG-I-like receptors (RLRs) play a major role in sensing RNA virus infection to initiate and modulate antiviral immunity. They interact with particular viral RNAs, most of them being still unknown.
Raul Y Sanchez David +10 more
doaj +1 more source
Special Issue “Function and Structure of Viral Ribonucleoproteins Complexes”
Viruses, 2020 RNA viruses are extraordinary evolution machines that efficiently ensure their replication by taking advantage of the association with viral and cellular components to form ribonucleic complexes (vRNPs) [...]
Serena Bernacchi
doaj +1 more source
Cajal body proteins differentially affect the processing of box C/D scaRNPs. [PDF]
PLoS ONE, 2015 Small nuclear ribonucleoproteins (snRNPs), which are required for pre-mRNA splicing, contain extensively modified snRNA. Small Cajal body-specific ribonucleoproteins (scaRNPs) mediate these modifications.
Isioma I Enwerem +3 more
doaj +1 more source