Results 21 to 30 of about 22,188 (264)
Nature Communications, 2021 Biogenesis of small nucleolar RNAs ribonucleoproteins (snoRNPs) requires dedicated assembly machinery. Here, the authors show that a subset of snoRNP assembly factors interacts, genetically or directly, with factors modulating chromatin architecture ...
Benoît Bragantini +19 more
doaj +1 more source
Preparation of Cas9 Ribonucleoproteins for Genome Editing
Bio-Protocol, 2022 Genome editing by the delivery of pre-assembled Cas9 ribonucleoproteins (Cas9 RNP) is an increasingly popular approach for cell types that are difficult to manipulate genetically by the conventional plasmid and viral methods.
SW Lin, Viet Nguyen, Steven Lin
doaj +1 more source
Membrane permeabilizing amphiphilic peptide delivers recombinant transcription factor and CRISPR-Cas9/Cpf1 ribonucleoproteins in hard-to-modify cells. [PDF]
PLoS ONE, 2018 Delivery of recombinant proteins to therapeutic cells is limited by a lack of efficient methods. This hinders the use of transcription factors or Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) ribonucleoproteins to develop cell ...
Thomas Del'Guidice +12 more
doaj +1 more source
Comparative analysis of viral RNA signatures on different RIG-I-like receptors
eLife, 2016 The RIG-I-like receptors (RLRs) play a major role in sensing RNA virus infection to initiate and modulate antiviral immunity. They interact with particular viral RNAs, most of them being still unknown.
Raul Y Sanchez David +10 more
doaj +1 more source
Ribonucleoprotein Immunoprecipitation (RIP) Analysis [PDF]
BIO-PROTOCOL, 2020 RNAs and RNA-binding proteins (RBPs) can interact dynamically in ribonucleoprotein (RNP) complexes that play important roles in controlling gene expression programs. One of the powerful ways to investigate changes in the association of RNAs with an RBP of interest is by immunoprecipitation (IP) analysis of native RNPs.
Martindale, Jennifer +2 more
openaire +3 more sources
Efficient One-Step Knockout by Electroporation of Ribonucleoproteins Into Zona-Intact Bovine Embryos
Frontiers in Genetics, 2020 Somatic cell nuclear transfer or cytoplasm microinjection have been used to generate genome-edited farm animals; however, these methods have several drawbacks that reduce their efficiency. This study aimed to develop electroporation conditions that allow
Luiz Sergio Almeida Camargo +3 more
doaj +1 more source
The TROVE module: A common element in Telomerase, Ro and Vault ribonucleoproteins
BMC Bioinformatics, 2003 Background Ribonucleoproteins carry out a variety of important tasks in the cell. In this study we show that a number of these contain a novel module, that we speculate mediates RNA-binding. Results The TROVE module – Telomerase, Ro and Vault module – is
Bateman Alex, Kickhoefer Valerie
doaj +1 more source
Nature Communications, 2020 Heterogeneous nuclear ribonucleoproteins (hnRNPs) play critical roles in the biogenesis, localization and transport of RNA. Here authors investigate a role for hnRNPs in sterol metabolism in mice and provide insights into their role in selective promoter
Zhengyi Zhang +14 more
doaj +1 more source
The World of Stable Ribonucleoproteins and Its Mapping With Grad-Seq and Related Approaches
Frontiers in Molecular Biosciences, 2021 Macromolecular complexes of proteins and RNAs are essential building blocks of cells. These stable supramolecular particles can be viewed as minimal biochemical units whose structural organization, i.e., the way the RNA and the protein interact with each
Milan Gerovac +4 more
doaj +1 more source
Journal of Immunology Research, 2017 The goal of the present study was to determine whether peptidylarginine deiminase PAD2 and PAD4 enzymes are present in Balb/c mouse salivary glands and whether they are able to citrullinate Ro and La ribonucleoproteins.
Mayra Rodríguez-Rodríguez +7 more
doaj +1 more source