Results 61 to 70 of about 24,238 (294)

Viral Infection‐Inspired Autonomous Detection of Fusion‐Competent Viruses for Screening and Environmental Surveillance

Advanced Materials, EarlyView.
Inspired by viral entry mechanisms, the FUSION assay enables autonomous detection of respiratory viruses via membrane fusion–triggered CRISPR‐Cas13a activation. VEACON selectively fuses with fusion‐competent viruses, triggering fluorescence within confined vesicles.
Jae Chul Park, Yidam Song, Hyun‐Woo Choi, Jaeuk Sung, Harin Jin, WonSeok Gwak, Kirim Yoo, Soohwang Lee, Jongeon Park, Jeongmin Kim, Hye‐Jun Jo, Jahyun Koo, Youngdo Jeong, Kwan Hyi Lee, Seung‐Jung Kee, Hojun Kim   +15 more
wiley   +1 more source

PRC2.1 Coordinates Peri‐Nucleolar H3K27me3‐Enriched Heterochromatin Organization and NPM1 Pentamerization to Maintain Nucleolar Integrity

Advanced Science, EarlyView.
PRC2.1(PCL2)‐coordinated H3K27me3‐enriched PNH establishes a spatial scaffold crucial for nucleolar integrity. As a crucial coordinator, PCL2 links PRC2.1 to chromatin organization and NPM1 assembly. This network‐based model reveals how chromatin modifications and nucleolar components cooperatively maintain nucleolar architecture, revealing novel ...
Lina Zhu, Wenqin Wang, Shuyun Chen, Gangyi Zhu, Zhen Wu, Yue Gu, Jingwen Xiong, Xu Zhang, Heng Liu, Kyoichi Isono, Youming Zhang, Jingjing Cao, Xiangzhi Li   +12 more
wiley   +1 more source

Chloroplast ribonucleoproteins are associated with both mRNAs and intron-containing precursor tRNAs

, 1999
Tobacco chloroplasts possess five conserved ribonucleoproteins (cpRNPs). To elucidate the function of cpRNPs we analyzed their localization and target nucleic acid molecules in chloroplasts.
Takahiro Nakamura, Masahiro Sugiura, Masaru Ohta, Ohta, Masaru, Mamoru Sugita, Sugita, Mamoru, Nakamura, Takahiro, Sugiura, Masahiro   +7 more
core   +1 more source

G3BP1 Succinylation at K413 is Critical for Cardiac Function by Modulating PI3K‐AKT‐mTOR Signal Axis

Advanced Science, EarlyView.
Schematic illustrating the impact of G3BP1 succinylation at K413 on cardiac function. In the healthy human heart, G3BP1 succinylation maintains homeostatic mTOR signaling. In patients with dilated cardiomyopathy (DCM) and heart failure (HF), G3BP1 de‐succinylation induces RagA expression and disrupts the binding of the TSC1/2 complex, leading to the ...
Yuan Zhang, Cancan Yao, Yan Chen, Ke Cai, Zhouping Lu, Boxuan Wu, Kun Yu, Yan Shi, Jianyuan Zhao, Xiangyu Zhou   +9 more
wiley   +1 more source

Protocol for the electroporation of CRISPR-Cas for DNA and RNA targeting in Bos taurus zygotes

STAR Protocols
Summary: The use of CRISPR-Cas9 ribonucleoproteins has revolutionized manipulation of genomes. Here, we present a protocol for the electroporation of CRISPR-Cas for DNA and RNA targeting in Bos taurus zygotes.
Fernando H. Biase, Gustavo Schettini
doaj   +1 more source

Influenza virus genome reaches the plasma membrane via a modified endoplasmic reticulum and Rab11-dependent vesicles

Nature Communications, 2017
Transport of neo-synthesized influenza A virus viral ribonucleoproteins (vRNPs) from the nucleus to the plasma membrane involves Rab 11 but the mechanism is unclear.
Isabel Fernández de Castro Martin, Guillaume Fournier, Martin Sachse, Javier Pizarro-Cerda, Cristina Risco, Nadia Naffakh   +5 more
doaj   +1 more source

Generation of replication-proficient Influenza Virus NS1 point mutants with interferon-hyperinducer phenotype

, 2014
The NS1 protein of influenza A viruses is the dedicated viral interferon (IFN)-antagonist. Viruses lacking NS1 protein expression cannot multiply in normal cells but are viable in cells deficient in their ability to produce or respond to IFN.
Fernández, Yolanda, Juan Ortín, Randall, Richard E, Richard E. Randall (190211), Killip, Marian J, Víctor J Asensio, Bengoechea, José A, Pérez-Cidoncha, M, Marian J. Killip (571160), Marian J Killip, Maite Pérez-Cidoncha (195699), Ortín, Juan, Killip, MJ, Ortín, J, Richard E Randall, Juan Ortín (5649007), Yolanda Fernández, Fernández, Y, Asensio, Víctor J, Randall, RE, José A Bengoechea, José A. Bengoechea (124595), Pérez-Cidoncha, Maite, Bengoechea, JA, Víctor J. Asensio (571161), Bengoechea, José A; id_orcid, Maite Pérez-Cidoncha, Asensio, VJ, Yolanda Fernández (268684)   +28 more
core   +1 more source

Nucleocapsid protein structures from orthobunyaviruses reveal insight into ribonucleoprotein architecture and RNA polymerization [PDF]

, 2013
All orthobunyaviruses possess three genome segments of single-stranded negative sense RNA that are encapsidated with the virus-encoded nucleocapsid (N) protein to form a ribonucleoprotein (RNP) complex, which is uncharacterized at high resolution.
Stonehouse, N. J., al., Et, Ranson, N. A., Hiscox, JA, Matthews, SV, Walter, C. T., Hiscox, J. A., Fooks, Anthony R., Walter, Cheryl T., Shepherd, DA, Ariza, Antonio, Shepherd, D. A., Elliott, R.M., Fooks, AR, Hiscox, Julian A., Dent, KC, Barr, John N., Edwards, T. A., Tanner, SJ, Dent, Kyle C., Matthews, S. V., Shepherd, D.A., Stonehouse, N.J., Barr, J. N., Dent, K.C., Barr, JN, Stonehouse, NJ, Hiscox, J.A., Tanner, S. J., Edwards, T.A., Stonehouse, Nicola J., Dent, K. C., Luo, Ming, Ashcroft, A.E., Shepherd, Dale A., Green, T. J., Ariza, A, Elliott, RM, Elliott, Richard M., Tanner, S.J., Tanner, Sian J., Ariza, A., Wu, W., Edwards, Thomas A., Matthews, Susan V., Ashcroft, Alison E., Wu, W, Green, Todd J., Green, TJ, Walter, C.T., Fooks, A. R., Fooks, A.R., Luo, M, Ashcroft, A. E., Ranson, Neil A., Luo, M., Walter, CT, Elliott, R. M., Green, T.J., Matthews, S.V., Ranson, N.A., Barr, J.N., Ashcroft, AE, Edwards, TA, Ranson, NA, Wu, Weining   +65 more
core   +1 more source

HNRNPU K181 Lactylation Drives Cervical Cancer Growth by Upregulating PHGDH and Reprogramming Serine Metabolism

Advanced Science, EarlyView.
Lactate in cervical cancer induces HNRNPU K181 lactylation, opposed by NAA50‐mediated acetylation and suppressed by Pazopanib. This lactylation enhances HNRNPU binding to PHGDH pre‐mRNA exon 1, maintaining exon 1‐containing transcripts and mRNA stability, thereby activating serine metabolism.
Chang Zhang, Qingfei Meng, Hui Jiao, Huixin Liu, Xiangchao Wang, Honglan Zhou, Yishu Wang   +6 more
wiley   +1 more source

NIR is degraded by the anaphase-promoting complex proteasome pathway [PDF]

Archives of Biological Sciences, 2014
Novel INHAT Repressor (NIR) is a histone acetylation inhibitor that can directly bind histone complexes and the tumor suppressors p53 and p63.
Jeong Ho Myong, Kang Kook Min, Kim Yang-Hee, Han Jin Seung   +3 more
doaj   +1 more source