Results 71 to 80 of about 25,962 (290)

Protocol for the electroporation of CRISPR-Cas for DNA and RNA targeting in Bos taurus zygotes

STAR Protocols
Summary: The use of CRISPR-Cas9 ribonucleoproteins has revolutionized manipulation of genomes. Here, we present a protocol for the electroporation of CRISPR-Cas for DNA and RNA targeting in Bos taurus zygotes.
Fernando H. Biase, Gustavo Schettini
doaj   +1 more source

Ionic Gating of Liquid‐Crystal‐Like Pteridine Assemblies Enables Tunable Light Scattering

Advanced Materials, EarlyView.
Tunable optical properties emerge through developmentally regulated ionic gating, which transforms disordered, ultraviolet (UV)‐absorbing organelles into concentrically ordered, liquid‐crystal‐like light scatterers. By integrating analyses of pteridine composition, potassium‐dependent assembly, hierarchical ultrastructure, and optical behavior, the ...
Sourabh Bera, Zohar Eyal, Yael Noy, Yuval Barzilay, Siddharth Sahoo, Srikant Moharana, Avi Baram, Tali Lerer‐Goldshtein, Neta Varsano, Bar Bader, Lothar Houben, Uwe Heinig, Iddo Pinkas, Moshe Goldsmith, Tamar Unger, Shira Albeck, Andrea Sorrentino, Maxim Itkin, Sergey Malitsky, Ziv Porat, Ido Hadar, Venkata Jayasurya Yallapragada, Dvir Gur   +22 more
wiley   +1 more source

catRAPID signature: identification of ribonucleoproteins and RNA-binding regions [PDF]

, 2016
MOTIVATION: Recent technological advances revealed that an unexpected large number of proteins interact with transcripts even if the RNA-binding domains are not annotated.
Livi, Carmen Maria, Klus, Petr, Delli Ponti, Riccardo, Tartaglia, Gian Gaetano, Delli Ponti, Riccardo, 1987-, Klus, Petr, 1985-   +5 more
core   +1 more source

Influenza virus genome reaches the plasma membrane via a modified endoplasmic reticulum and Rab11-dependent vesicles

Nature Communications, 2017
Transport of neo-synthesized influenza A virus viral ribonucleoproteins (vRNPs) from the nucleus to the plasma membrane involves Rab 11 but the mechanism is unclear.
Isabel Fernández de Castro Martin, Guillaume Fournier, Martin Sachse, Javier Pizarro-Cerda, Cristina Risco, Nadia Naffakh   +5 more
doaj   +1 more source

Pre‐Regenerative Endothelial Cells Empowered by Antler Blood Exosomes Orchestrate Peripheral Nerve Repair

Advanced Science, EarlyView.
The pre‐regenerative vascular niche (PVN) is essential for nerve repair, yet its endothelial blueprint remains unclear. We identified angiogenic ECs (AECs) as the dominant pre‐regenerative subset and found that antler blood–derived exosomes (AB‐EXO) promote repair via IMP3.
Jinsheng Huang, Qiang Chen, Aihua Jin, Xiaoqi Yang, Hui Zhou, Weiquan Gong, Jin Cheng, Jun Dai, Minjin Wang, Meihua Piao, Yuanyi Wang, Nan Zhou   +11 more
wiley   +1 more source

Smart Nanotechnologies for Multimodal Neuromodulation and Brain Interfacing

Advanced Science, EarlyView.
Recent advances in smart nanotechnologies are expanding the toolbox for brain interfacing, from wireless neuromodulation and high‐resolution sensing to targeted delivery within the central nervous system. By combining responsive nanomaterials with bioinspired design, these platforms enable multimodal interactions with neurons and glia, while also ...
Tommaso Curiale, Marie Celine Lefevre, Alessio Carmignani, Maria Cristina Ceccarelli, Matteo Battaglini, Attilio Marino, Gianni Ciofani   +6 more
wiley   +1 more source

Human heat shock protein 40 (Hsp40/DnaJB1) promotes influenza A virus replication by assisting nuclear import of viral ribonucleoproteins [PDF]

, 2016
A unique feature of influenza A virus (IAV) life cycle is replication of the viral genome in the host cell nucleus. The nuclear import of IAV genome is an indispensable step in establishing virus infection.
Batra, Jyoti, Tripathi, Shashank, Lal, Renu B., Sambhara, Suryaprakash, Kumar, Amrita, Katz, Jacqueline M., Lal, Sunil K., Cox, Nancy J.   +7 more
core   +1 more source

Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes

Nature Communications, 2017
Protocols for crop genome editing would ideally be quick, efficient and specific while avoiding integration of transgenes into the genome of edited plants. Here, Lianget al.
Zhen Liang, Kunling Chen, Tingdong Li, Yi Zhang, Yanpeng Wang, Qian Zhao, Jinxing Liu, Huawei Zhang, Cuimin Liu, Yidong Ran, Caixia Gao   +10 more
doaj   +1 more source

Engineering Microbial Particles for Next‐Generation Biomedical Platforms

Advanced Science, EarlyView.
Microbe‐derived particles (MDPs), which include extracellular vesicles, outer membrane vesicles, inclusion bodies, polysaccharide particles, and virus‐like particles, represent a rapidly expanding category of bioinspired nanomaterials. With their natural origin, intrinsic biocompatibility, and highly programmable functionality, MDPs serve as a ...
Yuting Li, Yunjie Lu, Ziyan Huang, Jianjiang Chen, Yanbin Liu, Lei Qin, Chao Wang, Yumin Wu   +7 more
wiley   +1 more source

NEDD4 controls spermatogonial stem cell homeostasis and stress response by regulating messenger ribonucleoprotein complexes

Nature Communications, 2017
Stress granules (SG) comprise aggregates of cellular messenger ribonucleoproteins (mRNPs) but how they form is unclear. Here, the authors identify NEDD4, an E3 ubiquitin ligase, as regulating the RNA binding protein NANOS2 and turnover of mRNP components,
Zhi Zhou, Hiroshi Kawabe, Atsushi Suzuki, Kaori Shinmyozu, Yumiko Saga   +4 more
doaj   +1 more source