Results 71 to 80 of about 24,238 (294)

Vesicular transport of a ribonucleoprotein to mitochondria [PDF]

Biology Open, 2014
ABSTRACT Intracellular trafficking of viruses and proteins commonly occurs via the early endosome in a process involving Rab5. The RNA Import Complex (RIC)-RNA complex is taken up by mammalian cells and targeted to mitochondria. Through RNA interference, it was shown that mito-targeting of the ribonucleoprotein (RNP) was dependent on ...
Joyita Mukherjee, Biraj Mahato, Samit Adhya   +2 more
openaire   +3 more sources

Human heat shock protein 40 (Hsp40/DnaJB1) promotes influenza A virus replication by assisting nuclear import of viral ribonucleoproteins [PDF]

, 2016
A unique feature of influenza A virus (IAV) life cycle is replication of the viral genome in the host cell nucleus. The nuclear import of IAV genome is an indispensable step in establishing virus infection.
Batra, Jyoti, Tripathi, Shashank, Lal, Renu B., Sambhara, Suryaprakash, Kumar, Amrita, Katz, Jacqueline M., Lal, Sunil K., Cox, Nancy J.   +7 more
core   +1 more source

Ultrasound‐Actuated Gene Editing in Human Kidney Organoids

Advanced Science, EarlyView.
Peptide‐stabilized nanoemulsions enable spatially controlled, ultrasound‐triggered delivery of gene‐editing proteins deep within human kidney organoid tissues. Focused acoustic pulses drive droplet vaporization, propelling cargo into target renal cells while preserving native tissue microarchitecture. This non‐viral platform improves both the depth and
Michael A. Miller, Nicole Vo, Utkarsh, Ivan Sokirniy, Justin Pritchard, Benjamin S. Freedman, Scott H. Medina   +6 more
wiley   +1 more source

NEDD4 controls spermatogonial stem cell homeostasis and stress response by regulating messenger ribonucleoprotein complexes

Nature Communications, 2017
Stress granules (SG) comprise aggregates of cellular messenger ribonucleoproteins (mRNPs) but how they form is unclear. Here, the authors identify NEDD4, an E3 ubiquitin ligase, as regulating the RNA binding protein NANOS2 and turnover of mRNP components,
Zhi Zhou, Hiroshi Kawabe, Atsushi Suzuki, Kaori Shinmyozu, Yumiko Saga   +4 more
doaj   +1 more source

The Structure and Function of Small Nucleolar Ribonucleoproteins

, 2007
Eukaryotes and archaea use two sets of specialized ribonucleoproteins (RNPs) to carry out sequencespecific methylation and pseudouridylation of RNA, the two most abundant types of modifications of cellular RNAs. In eukaryotes, these protein–RNA complexes
Reichow, Steve, Hamma, Tomoko, Varani, Gabriele, Ferré-D’Amaré, Adrian R.   +3 more
core   +1 more source

Genetic engineering in primary human B cells with CRISPR-Cas9 ribonucleoproteins [PDF]

, 2018
Genome editing in human cells with targeted nucleases now enables diverse experimental and therapeutic genome engineering applications, but extension to primary human B cells remains limited.
Kristina W. Rosbe, Alexander Marson, Zuniga-Pflucker, Juan Carlos, Marson, Alexander, Wu, Chung-An M, Baglaenko, Yuriy, Dario M. Ferri, Joan E. Wither, Brauer, Patrick, Theodore L. Roth, Patrick Brauer, Roth, Theodore L, Allen, Christopher DC, Christopher D.C. Allen, Chung-An M. Wu, Yuriy Baglaenko, Juan Carlos Zuniga-Pflucker, Ferri, Dario M, Wither, Joan E, Rosbe, Kristina W   +19 more
core   +1 more source

HNRNPD Induces Radioresistance in Nasopharyngeal Carcinoma by Sequestering GRAMD4 mRNA in Stress Granules

Advanced Science, EarlyView.
HNRNPD promotes radioresistance in nasopharyngeal carcinoma by enhancing stress granule assembly and sequestering GRAMD4 mRNA. This suppresses GRAMD4 translation and inhibits mitochondrial apoptosis. Targeting the integrated stress response with ISRIB restores GRAMD4 expression and sensitizes tumors to radiotherapy, revealing a translational control ...
Yingzi Li, Tong Xiang, Yuanyuan Liu, Desheng Weng, Jingjing Zhao, Hao Chen, Yan Tang, Songzuo Xie, Hao Zhang, Jun Luo, Xinyi Yang, Qiuzhong Pan, Muping Di, Jianchuan Xia   +13 more
wiley   +1 more source

Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes

Nature Communications, 2017
Protocols for crop genome editing would ideally be quick, efficient and specific while avoiding integration of transgenes into the genome of edited plants. Here, Lianget al.
Zhen Liang, Kunling Chen, Tingdong Li, Yi Zhang, Yanpeng Wang, Qian Zhao, Jinxing Liu, Huawei Zhang, Cuimin Liu, Yidong Ran, Caixia Gao   +10 more
doaj   +1 more source

Polyamine-Mediated Stoichiometric Assembly of Ribonucleoproteins for Enhanced mRNA Delivery

, 2019
Messenger RNA (mRNA) represents a promising class of nucleic acid drugs. Although numerous carriers have been developed for mRNA delivery, the inefficient mRNA expression inside cells remains a major challenge.
Wu, Connie, Li, Jiahe, Wang, Wade, Hong, Celestine, He, Yanpu, Hammond, Paula T   +5 more
core   +1 more source

UCHL3 Regulates Subgenomic Flaviviral RNA Condensates to Promote Virus Propagation

Advanced Science, EarlyView.
ABSTRACT Flavivirus subgenomic RNAs (sfRNAs) antagonise antiviral defences, yet how sfRNAs are organized and maintained in cells remains poorly understood. Here we identify ubiquitin C‐terminal hydrolase L3 (UCHL3) as a post‐translational regulator of flavivirus sfRNA stability and function.
Oscar Trejo‐Cerro, Anna Beekmayer‐Dhillon, Qi Wen Teo, Lewis Siu, Ming Yuan Li, Kate J. Heesom, Adán Pinto‐Fernández, Sumana Sanyal   +7 more
wiley   +1 more source