Results 91 to 100 of about 35,404 (208)

Comparison of CRISPR-Cas9/Cas12a Ribonucleoprotein Complexes for Genome Editing Efficiency in the Rice Phytoene Desaturase (OsPDS) Gene

Rice, 2020
Background Delivery of CRISPR reagents into cells as ribonucleoprotein (RNP) complexes enables transient editing, and avoids CRISPR reagent integration in the genomes.
Raviraj Banakar, Mollie Schubert, Michael Collingwood, Christopher Vakulskas, Alan L. Eggenberger, Kan Wang   +5 more
doaj   +1 more source

Cellular bases of the RNA metabolism dysfunction in motor neurons of a murine model of spinal muscular atrophy: Role of Cajal bodies and the nucleolus [PDF]

, 2017
Spinal muscular atrophy (SMA) is caused by a homozygous deletion or mutation in the survival motor neuron 1 (SMN1) gene that leads to reduced levels of SMN protein resulting in degeneration of motor neurons (MNs).
Berciano, Maria T., Calderó i Pardo, Jordi, Lafarga, Miguel, Narcís, Oriol J., Piedrafita Llorens, Lídia, Riancho, Javier, Tapia, Olga, Tarabal Mostazo, Olga   +7 more
core   +2 more sources

BATCH-GE : batch analysis of next-generation sequencing data for genome editing assessment [PDF]

, 2016
Targeted mutagenesis by the CRISPR/Cas9 system is currently revolutionizing genetics. The ease of this technique has enabled genome engineering in-vitro and in a range of model organisms and has pushed experimental dimensions to unprecedented proportions.
Boel, Annekatrien, Callewaert, Bert, Coucke, Paul, De Paepe, Anne, De Rocker, Nina, Menten, Björn, Steyaert, Wouter, Willaert, Andy   +7 more
core   +2 more sources

An in vitro network of intermolecular interactions between viral RNA segments of an avian H5N2 influenza A virus: comparison with a human H3N2 virus. [PDF]

, 2012
International audienceThe genome of influenza A viruses (IAV) is split into eight viral RNAs (vRNAs) that are encapsidated as viral ribonucleoproteins. The existence of a segment-specific packaging mechanism is well established, but the molecular basis ...
Cavalier, Annie, Fournier, Emilie, Gavazzi, Cyrille, Isel, Catherine, Lina, Bruno, Marquet, Roland, Moules, Vincent, Thomas, Daniel   +7 more
core   +3 more sources

Composition of the Survival Motor Neuron (SMN) complex in Drosophila melanogaster [PDF]

, 2018
Spinal Muscular Atrophy (SMA) is caused by homozygous mutations in the human survival motor neuron 1 (SMN1) gene. SMN protein has a well-characterized role in the biogenesis of small nuclear ribonucleoproteins (snRNPs), core components of the spliceosome.
Baillat, David, Droby, Gaith N., Gray, Kelsey M., Kelly, Jo A., Lamond, Angus I., Matera, A. Gregory, Raimer, Amanda C., Schmidt, Casey A., Ten Have, Sara, Wagner, Eric J.   +9 more
core   +3 more sources

Regulatory RNPs: a novel class of ribonucleoproteins that potentially contribute to ribosome heterogeneity

Biology Open, 2017
Many ribonucleoproteins (RNPs), which are comprised of noncoding RNA and associated proteins, are involved in essential cellular processes such as translation and pre-mRNA splicing.
Aaron R. Poole, Ian Vicino, Hironori Adachi, Yi-Tao Yu, Michael D. Hebert   +4 more
doaj   +1 more source

Multiple splicing factors are released from endogenous complexes during in vitro pre-mRNA splicing [PDF]

, 1989
Pre-mRNA splicing occurs in a macromolecular complex called the spliceosome. Efforts to isolate spliceosomes from in vitro splicing reactions have been hampered by the presence of endogenous complexes that copurify with de novo spliceosomes formed on ...
Conway, G. C., Krainer, A. R., Spector, D. L., Roberts, R. J.   +3 more
core  

Ribonucleoprotein Therapy in Alzheimer's Disease?

Aging, 2014
Age-related neurodegenerative pathologies like Alzheimer’s and Parkinson’s diseases are accelerated by a decline in neuronal homeostasis. In this issue of Cell Reports, Kang et al. [1] reports the post-transcriptional mechanisms that regulate the expression and processing of amyloid precursor protein (APP), a protein directly implicated in the ...
Je-Hyun, Yoon, Myriam, Gorospe
openaire   +2 more sources

Antibiotics and RNase P

Antibiotics, 2016
RNase P is an essential endonuclease in tRNA biogenesis, which generates the mature 5′-termini of tRNAs. Most forms of RNase P are ribonucleoproteins, i.e., they consist of an essential RNA and protein subunits.
Denis Drainas
doaj   +1 more source

Editorial: Regulation of Soluble Immune Mediators by Non-Coding RNAs

Frontiers in Immunology, 2020
Daniela Bosisio, Flavia Bazzoni
doaj   +1 more source