Results 81 to 90 of about 35,404 (208)

Enhanced NK-92 Cytotoxicity by CRISPR Genome Engineering Using Cas9 Ribonucleoproteins

Frontiers in Immunology, 2020
Natural killer (NK) cells are an attractive cell-type for adoptive immunotherapy, but challenges in preparation of therapeutic primary NK cells restrict patient accessibility to NK cell immunotherapy. NK-92 is a well-characterized human NK cell line that
Rih-Sheng Huang, Hsin-An Shih, Hsin-An Shih, Min-Chi Lai, Min-Chi Lai, Yao-Jen Chang, Steven Lin, Steven Lin   +7 more
doaj   +1 more source

Emerging Roles for hnRNPs in post-transcriptional regulation: what can we learn from flies? [PDF]

, 2014
Heterogeneous nuclear ribonucleoproteins (hnRNPs) are a highly conserved family of RNA-binding proteins able to associate with nascent RNAs in order to support their localization, maturation and translation.
CORONA, Davide, Onorati, MC, Piccolo, LL
core   +1 more source

Genome editing in primary cells and in vivo using viral-derived Nanoblades loaded with Cas9-sgRNA ribonucleoproteins

Nature Communications, 2019
A current challenge in genome editing is delivering Cas9 and sgRNA into target cells. Here the authors engineer a delivery system based on murine leukemia virus-like particles loaded with Cas9-sgRNA ribonucleoproteins to induce efficient genome editing ...
Philippe E. Mangeot, Valérie Risson, Floriane Fusil, Aline Marnef, Emilie Laurent, Juliana Blin, Virginie Mournetas, Emmanuelle Massouridès, Thibault J. M. Sohier, Antoine Corbin, Fabien Aubé, Marie Teixeira, Christian Pinset, Laurent Schaeffer, Gaëlle Legube, François-Loïc Cosset, Els Verhoeyen, Théophile Ohlmann, Emiliano P. Ricci   +18 more
doaj   +1 more source

Reprogramming human T cell function and specificity with non-viral genome targeting. [PDF]

, 2018
Decades of work have aimed to genetically reprogram T cells for therapeutic purposes1,2 using recombinant viral vectors, which do not target transgenes to specific genomic sites3,4.
Alkorta-Aranburu, Gorka, Ashworth, Alan, Bacchetta, Rosa, Carmody, David, Carnevale, Julia, Chen, Jeff W, Cho, Min, Del Gaudio, Daniela, Esensten, Jonathan H, Ferris, Andrea L, Greeley, Siri Atma W, Gurumurthy, Channabasavaiah B, Haugwitz, Michael, Herold, Kevan C, Hiatt, Joseph, Hughes, Stephen H, Krystofinski, Paige, Kupfer, Gary M, Lee, Michael R, Leonetti, Manuel D, Li, Han, Li, P Jonathan, Mao, Ying, Marson, Alexander, Matsumoto, Hiroyuki, May, Andrew P, Meffre, Eric, Morell, Montse, Nguyen, David N, Pellerin, Laurence, Puig-Saus, Cristina, Putnam, Amy L, Quadros, Rolen M, Ribas, Antoni, Romberg, Neil, Roncarolo, Maria Grazia, Roth, Theodore L, Saco, Justin, Schickel, Jean-Nicolas, Schumann, Kathrin, Shifrut, Eric, Smith, Baz, Tobin, Victoria, Weissman, Jonathan S, Yu, Ruby   +44 more
core  

Evidence that avian reovirus σNS is an RNA chaperone: implications for genome segment assortment. [PDF]

, 2015
Reoviruses are important human, animal and plant pathogens having 10-12 segments of double-stranded genomic RNA. The mechanisms controlling the assortment and packaging of genomic segments in these viruses, remain poorly understood.
Ault, J, Borodavka, A, Stockley, PG, Tuma, R   +3 more
core   +1 more source

A Cas9 Ribonucleoprotein Platform for Functional Genetic Studies of HIV-Host Interactions in Primary Human T Cells

Cell Reports, 2016
Summary: New genetic tools are needed to understand the functional interactions between HIV and human host factors in primary cells. We recently developed a method to edit the genome of primary CD4+ T cells by electroporation of CRISPR/Cas9 ...
Judd F. Hultquist, Kathrin Schumann, Jonathan M. Woo, Lara Manganaro, Michael J. McGregor, Jennifer Doudna, Viviana Simon, Nevan J. Krogan, Alexander Marson   +8 more
doaj   +1 more source

Systemic nanoparticle delivery of CRISPR-Cas9 ribonucleoproteins for effective tissue specific genome editing

Nature Communications, 2020
Therapeutic targets of CRISPR-Cas can often not be accessed due to lack of carriers to deliver RNPs systematically. Here, the authors engineer modified lipid nanoparticles for delivery of gene editing proteins to specific tissues.
Tuo Wei, Qiang Cheng, Yi-Li Min, Eric N. Olson, Daniel J. Siegwart   +4 more
doaj   +1 more source

Tah1 helix-swap dimerization prevents mixed Hsp90 co-chaperone complexes [PDF]

, 2015
Specific co-chaperone adaptors facilitate the recruitment of client proteins to the Hsp90 system. Tah1 binds the C-terminal conserved MEEVD motif of Hsp90, thus linking an eclectic set of client proteins to the R2TP complex for their assembly and ...
Ali, Back, Battye, Boulon, Chartron, Chrisostomos Prodromou, Eckert, Emsley, Forget, Gonzales, Hořejší, Itsuki, Izumi, Jiménez, Kakihara, Krissinel, Kurokawa, Laurence H. Pearl, Liou, Liu, Mohinder Pal, Morgan, Murshudov, Nagradova, Nyarko, Pal, Pearl, Polier, Prodromou, Prodromou, Rhodri M. L. Morgan, Roe, S. Mark Roe, Samarsky, Siligardi, Takai, Te, Tobaben, Tung, Vaughan, Worrall, Zhang, Zhao   +42 more
core   +1 more source

Ro60 and La ribonucleoproteins become self-aggregated by cell stress

Reumatismo, 2011
Ro is a cellular particle composed by three ribonucleoproteins of 60, 54 and 52 kDa (1-3). Ro60 forms a complex with one of the 1-5 hYRNAs (4). Antigenic properties of Ro were described by Clark in 1969 using autoimmune sera (5), and it is broadly ...
E. Avalos-Díaz, D. Herrera-van-Oostdam, S.H. Sánchez-Rodríguez, R. Herrera-Esparza   +3 more
doaj   +1 more source

Influenza virus morphogenesis and budding. [PDF]

, 2009
Influenza viruses are enveloped, negative stranded, segmented RNA viruses belonging to Orthomyxoviridae family. Each virion consists of three major sub-viral components, namely (i) a viral envelope decorated with three transmembrane proteins ...
Balogun, Rilwan A, Barman, Subrata, Nayak, Debi P, Yamada, Hiroshi, Zhou, Z Hong   +4 more
core   +1 more source