Results 111 to 120 of about 59,620 (205)
Clinical Genetics, Volume 109, Issue 4, Page 772-777, April 2026.CRISPR activation of NEB in fibroblasts, followed by RNA‐sequencing, documents spliceogenic effects of a NEB intronic variant. The assay enabled variant reclassification as likely pathogenic, providing molecular diagnosis in fetuses with Arthrogryposis multiplex congenita 6.
Doriana Misceo +7 more
wiley +1 more source
Capturing the ‘ome’ : the expanding molecular toolbox for RNA and DNA library construction [PDF]
, 2018 All sequencing experiments and most functional genomics screens rely on the generation of libraries to comprehensively capture pools of targeted sequences.
Boone, Morgane +2 more
core +2 more sources
The SMC Loader Scc2 Promotes ncRNA Biogenesis and Translational Fidelity.
PLoS Genetics, 2015 The Scc2-Scc4 complex is essential for loading the cohesin complex onto DNA. Cohesin has important roles in chromosome segregation, DSB repair, and chromosome condensation. Here we report that Scc2 is important for gene expression in budding yeast.
Musinu Zakari +5 more
doaj +1 more source
Non-canonical Translation in Plant RNA Viruses [PDF]
, 2017 Viral protein synthesis is completely dependent upon the host cell's translational machinery. Canonical translation of host mRNAs depends on structural elements such as the 5′ cap structure and/or the 3′ poly(A) tail of the mRNAs.
Manuel Miras +3 more
core +1 more source
Eukaryotic translation initiation machinery can operate in a prokaryotic-like mode without eIF2 [PDF]
, 2008 Unlike prokaryotes, a specialized eukaryotic initiation factor 2 (eIF2), in the form of the ternary complex eIF2*GTP*Met-tRNAiMet is utilized to deliver the initiator tRNA to the ribosome within all eukaryotic cells1.
Dmitri Andreev +3 more
core +1 more source
A barnavirus sequence mined from a transcriptome of the Antarctic pearlwort Colobanthus quitensis. [PDF]
, 2018 Because so few viruses in the family Barnaviridae have been reported, we searched for more of them in public sequence databases. Here, we report the complete coding sequence of Colobanthus quitensis associated barnavirus 1, mined from a transcriptome of ...
Bertini, Laura +5 more
core +2 more sources
Orthoretroviral-like prototype foamy virus gag-pol expression is compatible with viral replication [PDF]
, 2011 Background Foamy viruses (FVs) unlike orthoretroviruses express Pol as a separate precursor protein and not as a Gag-Pol fusion protein. A unique packaging strategy, involving recognition of briding viral RNA by both Pol precursor and Gag as well as ...
Anka Swiersy +4 more
core +2 more sources
Nature CommunicationsRNA post-transcriptional modifications act by stabilizing the functional conformations of RNA. While their role in messenger RNA (mRNA) decoding is well established, it is less clear how transfer RNA (tRNA) modifications outside the anticodon contribute ...
Evelyn M. Kimbrough +9 more
doaj +1 more source
Complete genome sequence of human astrovirus genotype 6 [PDF]
, 2010 Background Human astroviruses (HAstVs) are one of the important causes of acute gastroenteritis in children. Currently, eight HAstV genotypes have been identified and all but two (HAstV-6 and HAstV-7) have been fully sequenced.
Li Guo +6 more
core +1 more source
Exploiting preQ1 Riboswitches To Regulate Ribosomal Frameshifting
ACS Chemical Biology, 2013 Knowing the molecular details of the interaction between riboswitch aptamers and their corresponding metabolites is important to understand gene expression. Here we report on a novel in vitro assay to study preQ(1) riboswitch aptamers upon binding of 7-aminomethyl-7-deazaguanine (preQ(1)).
Yu, C.H. +3 more
openaire +3 more sources