Results 61 to 70 of about 343,033 (331)

Mass spectrometry of ribosomes and ribosomal subunits [PDF]

Proceedings of the National Academy of Sciences, 1998
Nanoflow electrospray ionization has been used to introduce intact Escherichia coli ribosomes into the ion source of a mass spectrometer. Mass spectra of remarkable quality result from a partial, but selective, dissociation of the particles within the mass spectrometer.
Benjamin, DR, Robinson, C, Hendrick, J, Hartl, F, Dobson, C   +4 more
openaire   +3 more sources

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

The mechanism of error induction by the antibiotic viomycin provides insight into the fidelity mechanism of translation

eLife, 2019
Applying pre-steady state kinetics to an Escherichia-coli-based reconstituted translation system, we have studied how the antibiotic viomycin affects the accuracy of genetic code reading.
Mikael Holm, Chandra Sekhar Mandava, Måns Ehrenberg, Suparna Sanyal   +3 more
doaj   +1 more source

Finding sense in the context

eLife, 2020
Ribosomal profiling has shed new light on how ribosomes can ignore stop codons in messenger RNA.
Kim M Keeling, David M Bedwell
doaj   +1 more source

Extensive evolution of cereal ribosome-inactivating proteins translates into unique structural features, activation mechanisms, and physiological roles [PDF]

, 2017
Ribosome-inactivating proteins (RIPs) are a class of cytotoxic enzymes that can depurinate rRNAs thereby inhibiting protein translation. Although these proteins have also been detected in bacteria, fungi, and even some insects, they are especially ...
De Zaeytijd, Jeroen, Van Damme, Els
core   +2 more sources

In Vitro Reconstitution of Yeast Translation System Capable of Synthesizing Long Polypeptide and Recapitulating Programmed Ribosome Stalling [PDF]

, 2021
Riku Nagai, Yichen Xu, Chang Liu, Ayaka Shimabukuro, Nono Takeuchi-Tomita   +4 more
openalex   +1 more source

Reduced vascular leakage correlates with breast carcinoma T regulatory cell infiltration but not with metastatic propensity

Molecular Oncology, EarlyView.
A mouse model for vascular normalization and a human breast cancer cohort were studied to understand the relationship between vascular leakage and tumor immune suppression. For this, endothelial and immune cell RNAseq, staining for vascular function, and immune cell profiling were employed.
Liqun He, Chiara Testini, Neda Hekmati, Altea Bonello, Aglaia Schiza, Emmanuel Nwadozi, Mia Phillipson, Carina Strell, Michael Welsh   +8 more
wiley   +1 more source

Mutational analysis of ribosomal proteins in a cohort of pediatric patients with T-cell acute lymphoblastic leukemia reveals Q123R, a novel mutation in RPL10

Frontiers in Genetics, 2022
T-cell acute lymphoblastic leukemia (T-ALL) is a subtype of ALL involving the malignant expansion of T-cell progenitors. It is driven by a number of different possible genetic lesions, including mutations in genes encoding for ribosomal proteins (RPs ...
Lorenza Bacci, Lorenza Bacci, Valentina Indio, Guglielmo Rambaldelli, Cristina Bugarin, Franco Magliocchetti, Franco Magliocchetti, Alberto Del Rio, Alberto Del Rio, Daniela Pollutri, Daniela Pollutri, Fraia Melchionda, Andrea Pession, Marina Lanciotti, Carlo Dufour, Giuseppe Gaipa, Lorenzo Montanaro, Lorenzo Montanaro, Marianna Penzo, Marianna Penzo   +19 more
doaj   +1 more source

HSP70 governs permeability and mechanotransduction in primary human endothelial cells

FEBS Open Bio, EarlyView.
HSP70 chemical inhibition reduces endothelial cell proliferation and increases permeability, the latter supported by normal interendothelial junctional protein distribution. HSP70 also plays a role in shear stress response, a hemodynamic force naturally present in blood vessels and correlated with vessel protection.
Andrea Pinto‐Martinez, Everton G. Melo, Isadora C. B. Pavan, Percíllia V. S. Oliveira, Luiza B. C. T. Coimbra, Thaís L. S. Araujo   +5 more
wiley   +1 more source

Thermodynamic and kinetic insights into stop codon recognition by release factor 1. [PDF]

, 2014
Stop codon recognition is a crucial event during translation termination and is performed by class I release factors (RF1 and RF2 in bacterial cells). Recent crystal structures showed that stop codon recognition is achieved mainly through a network of ...
Joseph, Simpson, Mathew, Merrill A, Trappl, Krista   +2 more
core