Results 71 to 80 of about 320,867 (304)

Mass spectrometry of ribosomes and ribosomal subunits [PDF]

Proceedings of the National Academy of Sciences, 1998
Nanoflow electrospray ionization has been used to introduce intact Escherichia coli ribosomes into the ion source of a mass spectrometer. Mass spectra of remarkable quality result from a partial, but selective, dissociation of the particles within the mass spectrometer.
Benjamin, DR, Robinson, C, Hendrick, J, Hartl, F, Dobson, C   +4 more
openaire   +3 more sources

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

FEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman, Katarzyna Stapor, Leszek Konieczny, Krzysztof Gądek, Piotr Nowakowski   +4 more
wiley   +1 more source

Bacterial riboproteogenomics : the era of N-terminal proteoform existence revealed [PDF]

, 2020
With the rapid increase in the number of sequenced prokaryotic genomes, relying on automated gene annotation became a necessity. Multiple lines of evidence, however, suggest that current bacterial genome annotations may contain inconsistencies and are ...
Fijalkowska, Daria, Fijalkowski, Igor, Van Damme, Petra, Willems, Patrick   +3 more
core   +2 more sources

The mechanism of error induction by the antibiotic viomycin provides insight into the fidelity mechanism of translation

eLife, 2019
Applying pre-steady state kinetics to an Escherichia-coli-based reconstituted translation system, we have studied how the antibiotic viomycin affects the accuracy of genetic code reading.
Mikael Holm, Chandra Sekhar Mandava, Måns Ehrenberg, Suparna Sanyal   +3 more
doaj   +1 more source

Finding sense in the context

eLife, 2020
Ribosomal profiling has shed new light on how ribosomes can ignore stop codons in messenger RNA.
Kim M Keeling, David M Bedwell
doaj   +1 more source

Mutational analysis of ribosomal proteins in a cohort of pediatric patients with T-cell acute lymphoblastic leukemia reveals Q123R, a novel mutation in RPL10

Frontiers in Genetics, 2022
T-cell acute lymphoblastic leukemia (T-ALL) is a subtype of ALL involving the malignant expansion of T-cell progenitors. It is driven by a number of different possible genetic lesions, including mutations in genes encoding for ribosomal proteins (RPs ...
Lorenza Bacci, Lorenza Bacci, Valentina Indio, Guglielmo Rambaldelli, Cristina Bugarin, Franco Magliocchetti, Franco Magliocchetti, Alberto Del Rio, Alberto Del Rio, Daniela Pollutri, Daniela Pollutri, Fraia Melchionda, Andrea Pession, Marina Lanciotti, Carlo Dufour, Giuseppe Gaipa, Lorenzo Montanaro, Lorenzo Montanaro, Marianna Penzo, Marianna Penzo   +19 more
doaj   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Intercompartmental communication in senescence

FEBS Open Bio, EarlyView.
Senescent cells experience structural changes in the plasma membrane, endoplasmic reticulum, mitochondria, lysosomes, nucleus, and cytoskeleton. These alterations disrupt crosstalk among cellular compartments, impairing vesicular trafficking, contact sites, and molecular flow.
Krystyna Mazan‐Mamczarz, Eleanor J. Wind, Jixiang Leng, Myriam Gorospe   +3 more
wiley   +1 more source

Evolution of plant ribosome-inactivating proteins [PDF]

, 2010
This contribution presents an updated analysis of the evolution of ribosome-inactivating proteins (RIPs) in plants. All evidence suggests that an ancestor of modern seed plants developed the RIP domain at least 300 million years ago.
Peumans, Willy J, Van Damme, Els
core   +1 more source

Mutant NPM1 in Acute Myeloid Leukemia Initiation and Maintenance

Aging and Cancer, EarlyView.
NPM1 mutations drive acute myeloid leukemia by acting as neomorphic transcriptional regulators that cooperate with Menin–MLL and XPO1 to sustain HOX/MEIS1 expression and block differentiation. Targeting these mutant‐specific transcriptional dependencies provides a rational therapeutic strategy for NPM1‐mutated AML.
Yanan Jiang, Zhong Fan, Runmeng Liu, Xiaotian Zhang   +3 more
wiley   +1 more source