Results 61 to 70 of about 247,874 (255)

Disruption of ribosome biogenesis triggers a p21/p53-mediated cell cycle checkpoint [PDF]

, 2006
Cell cycle entry requires a dramatic increase in protein production. In order to cope with this demand, the cell must upregulate ribosome biogenesis. Given that ribosome biogenesis is the most energy-consuming anabolic process in a growing cell, and ...
Di Cara, Alessandro
core   +1 more source

A role for eIF5A in translational control of stress‐inducible proteins in multiple myeloma cell lines

Molecular Oncology, EarlyView.
Loss of eIF5A rewires thapsigargin‐mediated endoplasmic reticulum stress responses in multiple myeloma cells by selective translation of mRNA transcripts, consequently dampening thapsigargin‐induced unfolded protein response (UPR) activation and shifting the balance from induction of apoptosis toward cell survival and proliferation.
Demi Both, Ingrid A. M. Derks, Raphaël Vanleyssem, Marine Leclercq, Najla El Hachem, Pierre Close, Eric Eldering, Marco Vincent Haselager   +7 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

SecM-stalled ribosomes adopt an altered geometry at the peptidyl transferase center [PDF]

, 2011
As nascent polypeptide chains are synthesized, they pass through a tunnel in the large ribosomal subunit. Interaction between specific nascent chains and the ribosomal tunnel is used to induce translational stalling for the regulation of gene expression.
Roland Beckmann, Daniel N. Wilson, Wilson, D., Wilson Daniel N., Mielke Thorsten, Jens Frauenfeld, Thorsten Mielke, Berninghausen, O., Mielke, T., Hoffmann, T., Beckmann, R., Wilson, Daniel N, Frauenfeld, Jens, Seidelt, B., Birgit Seidelt, Berninghausen, Otto, Berninghausen Otto, Frauenfeld Jens, Bhushan, S., Mielke, Thorsten, Shashi Bhushan, Hoffmann Thomas, Seidelt, Birgit, Bhushan, Shashi, Hoffmann, Thomas, Thomas Hoffmann, Otto Berninghausen, Beckmann, Roland, Seidelt Birgit, Beckmann Roland, Daniel N Wilson, Frauenfeld, J., Bhushan Shashi, Beckmann, R, Seidelt, B, Berninghausen, O, Mielke, T, Wilson, DN, Hoffmann, T, Frauenfeld, J,, Bhushan, S   +40 more
core   +2 more sources

Molecular Dynamics Simulations Suggest a Non-Doublet Decoding Model of −1 Frameshifting by tRNA^Ser3

Biomolecules, 2019
In-frame decoding in the ribosome occurs through canonical or wobble Watson−Crick pairing of three mRNA codon bases (a triplet) with a triplet of anticodon bases in tRNA.
Thomas Caulfield, Matt Coban, Alex Tek, Samuel Coulbourn Flores   +3 more
doaj   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source

Aging in Rats Differentially Affects Markers of Transcriptional and Translational Capacity in Soleus and Plantaris Muscle

Frontiers in Physiology, 2017
Alterations in transcriptional and translational mechanisms occur during skeletal muscle aging and such changes may contribute to age-related atrophy. Herein, we examined markers related to global transcriptional output (i.e., myonuclear number, total ...
Christopher B. Mobley, Petey W. Mumford, Wesley C. Kephart, Cody T. Haun, Angelia M. Holland, Darren T. Beck, Darren T. Beck, Jeffrey S. Martin, Jeffrey S. Martin, Kaelin C. Young, Kaelin C. Young, Richard G. Anderson, Romil K. Patel, Gillis L. Langston, Ryan P. Lowery, Ryan P. Lowery, Jacob M. Wilson, Michael D. Roberts, Michael D. Roberts   +18 more
doaj   +1 more source

Malformin A1–mediated cytotoxicity in ovarian cancer cells occurs through pyroptosis and autophagy

FEBS Open Bio, EarlyView.
This study investigated the effects of the natural compound Malformin A1 (MA1) on the cytoskeleton that regulates cell proliferation and migration. Disruption of the cytoskeleton can impair these processes and promote cancer cell death. MA1 disrupted cytoskeletal organization, induced DNA damage, inflammation, activated autophagy, and pyroptosis ...
Nada Abdullah Hassan, Ikram A. Burney, Shika Hanif Malgundkar, Siva Ramamoorthy, Ressin Varghese, Mohamed A. Al‐Kindi, Hamad Al Riyami, Shadia Al Bahlani, Sergey Dobretsov, Syed Imran Hassan, Benjamin K. Tsang, Yahya Tamimi   +11 more
wiley   +1 more source

Translatome and Transcriptome Profiling of Hypoxic-Induced Rat Cardiomyocytes

Molecular Therapy: Nucleic Acids, 2020
Adult cardiac hypoxia as a crucial pathogenesis factor can induce detrimental effects on cardiac injury and dysfunction. The global transcriptome and translatome reflecting the cellular response to hypoxia have not yet been extensively studied in ...
Zhijie Shen, Lixiong Zeng, Zhihui Zhang
doaj   +1 more source