Results 81 to 90 of about 343,033 (331)

RNA polymerase I inhibition induces terminal differentiation, growth arrest, and vulnerability to senolytics in colorectal cancer cells

Molecular Oncology, 2022
Ribosomal biogenesis and protein synthesis are deregulated in most cancers, suggesting that interfering with translation machinery may hold significant therapeutic potential. Here, we show that loss of the tumor suppressor adenomatous polyposis coli (APC)
Christoph Otto, Carolin Kastner, Stefanie Schmidt, Konstantin Uttinger, Apoorva Baluapuri, Sarah Denk, Mathias T. Rosenfeldt, Andreas Rosenwald, Florian Roehrig, Carsten P. Ade, Christina Schuelein‐Voelk, Markus E. Diefenbacher, Christoph‐Thomas Germer, Elmar Wolf, Martin Eilers, Armin Wiegering   +15 more
doaj   +1 more source

Full-length prepro-alpha-factor can be translocated across the mammalian microsomal membrane only if translation has not terminated. [PDF]

, 1988
We have previously shown that fully synthesized prepro-alpha-factor (pp alpha F), the precursor for the yeast pheromone alpha-factor, can be translocated posttranslationally across yeast rough microsomal (RM) membranes from a soluble, ribosome-free pool.
Garcia, PD, Walter, P
core  

Atomic mutagenesis of stop codon nucleotides reveals the chemical prerequisites for release factor-mediated peptide release. [PDF]

, 2018
Termination of protein synthesis is triggered by the recognition of a stop codon at the ribosomal A site and is mediated by class I release factors (RFs).
Clementi, Nina, Erlacher, Matthias David, Faserl, Klaus, Gasser, Catherina, Hoernes, Thomas Philipp, Hüttenhofer, Alexander, Joseph, Simpson, Juen, Michael Andreas, Kreutz, Christoph, Lindner, Herbert, Shi, Xinying, Willi, Jessica   +11 more
core   +3 more sources

HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein

FEBS Open Bio, EarlyView.
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley   +1 more source

Aging in Rats Differentially Affects Markers of Transcriptional and Translational Capacity in Soleus and Plantaris Muscle

Frontiers in Physiology, 2017
Alterations in transcriptional and translational mechanisms occur during skeletal muscle aging and such changes may contribute to age-related atrophy. Herein, we examined markers related to global transcriptional output (i.e., myonuclear number, total ...
Christopher B. Mobley, Petey W. Mumford, Wesley C. Kephart, Cody T. Haun, Angelia M. Holland, Darren T. Beck, Darren T. Beck, Jeffrey S. Martin, Jeffrey S. Martin, Kaelin C. Young, Kaelin C. Young, Richard G. Anderson, Romil K. Patel, Gillis L. Langston, Ryan P. Lowery, Ryan P. Lowery, Jacob M. Wilson, Michael D. Roberts, Michael D. Roberts   +18 more
doaj   +1 more source

The Anisotropic Adsorption of De Novo Allosteric Two‐Component Protein Fibers on Mica Surfaces

Advanced Functional Materials, EarlyView.
In this study, the interfacial behavior of de novo designed proteins that self‐assemble into tubular architectures with distinct morphologies — small (S), large (L), and helical (H) fibers — at the muscovite mica‐water interface is explored using in situ AFM. Abstract Protein adsorption at solid–liquid interfaces underlies many biomedical and materials
Chenyang Shi, Hao Shen, Yuna Bae, Jaeyoung Heo, Shuai Zhang, Lehan Yao, David Baker, James J De Yoreo   +7 more
wiley   +1 more source

Translatome and Transcriptome Profiling of Hypoxic-Induced Rat Cardiomyocytes

Molecular Therapy: Nucleic Acids, 2020
Adult cardiac hypoxia as a crucial pathogenesis factor can induce detrimental effects on cardiac injury and dysfunction. The global transcriptome and translatome reflecting the cellular response to hypoxia have not yet been extensively studied in ...
Zhijie Shen, Lixiong Zeng, Zhihui Zhang
doaj   +1 more source

Translation termination depends on the sequential ribosomal entry of eRF1 and eRF3.

, 2019
Translation termination requires eRF1 and eRF3 for polypeptide-and tRNA-release on stop codons. Additionally, Dbp5/DDX19 and Rli1/ABCE1 are required; however, their function in this process is currently unknown.
Beissel , C., Hampe , I., Karki, P., Krebber, H., Neumann, B., Uhse, S.   +5 more
core   +1 more source

Concerted modification of nucleotides at functional centers of the ribosome revealed by single-molecule RNA modification profiling [PDF]

, 2021
Andrew D. Bailey, Jason Talkish, Hongxu Ding, Haller Igel, Alejandra Durán, Shreya Mantripragada, Benedict Paten, Manuel Ares   +7 more
openalex   +1 more source

Intrinsically Disordered Protein‐Inspired Nanovector‐Based Coacervates for the Direct Cytosolic Transport of Biomacromolecules

Advanced Materials, EarlyView.
Intrinsically disordered protein‐inspired nanovectors (IDP‐NVs) form stable nanocoacervates (NCs) with diverse biomacromolecules. In situ conformational changes confer stability and adaptability to NCs under dynamically changing physiological conditions.
Soyeong Jin, Hyemin Park, Seuk‐Min Ryu, Dagyeong Guk, Jaeeun Lee, Seongeon Jin, Changjoon Keum, Jinyoung Park, Myoung‐Hwan Park, Chaekyu Kim, Hojun Kim, Jaegeun Noh, Kwan Hyi Lee, Ja‐Hyoung Ryu, Youngdo Jeong   +14 more
wiley   +1 more source