Results 211 to 220 of about 30,415 (253)

Gypsophilin, a New Type 1 Ribosome-inactivating Protein fromGypsophila elegans: Purification, Enzymatic Characterization, and Subcellular Localization

Bioscience, Biotechnology, and Biochemistry, 1997
Gypsophila elegans contains a new type 1 ribosome-inactivating protein, which we named gypsophilin. The protein was purified to apparent homogeneity by (NH4)2SO4 fractionation, ion-exchange chromatography, and adsorption chromatography. The protein was found to have a molecular mass of 28.0 kDa and a pI of about 10.1.
S, Yoshinari, S, Koresawa, S, Yokota, H, Sawamoto, M, Tamura, Y, Endo   +5 more
openaire   +2 more sources

Structure/funcion studies on type 1 ribosome inactivating proteins.

2008
Ribosome inactivating proteins (RIPs) are toxins, which depurinate major RNA inhibiting protein synthesis1. The cleaved N-glycosidic bond corresponds to a specific adenine located in the sarcin/ricin loop, highly conserved in rRNAs from different species and essential for interaction with elongation factors.
FERMANI, SIMONA, TOSI, GIOVANNA, FARINI, VALENTINA, POLITO, LETIZIA, FALINI, GIUSEPPE, RIPAMONTI, ALBERTO, BARBIERI, LUIGI, A. Chambery, BOLOGNESI, ANDREA   +8 more
openaire   +1 more source

Atomic resolution structure of cucurmosin, a novel type 1 ribosome-inactivating protein from the sarcocarp of Cucurbita moschata

Journal of Structural Biology, 2008
A novel type 1 ribosome-inactivating protein (RIP) designated cucurmosin was isolated from the sarcocarp of Cucurbita moschata (pumpkin). Besides rRNA N-glycosidase activity, cucurmosin exhibits strong cytotoxicities to three cancer cell lines of both human and murine origins, but low toxicity to normal cells.
Xiaomin, Hou, Edward J, Meehan, Jieming, Xie, Mingdong, Huang, Minghuang, Chen, Liqing, Chen   +5 more
openaire   +2 more sources

BIOACTIVITIES OF SoRIP1, A TYPE 1 RIBOSOME INACTIVATING PROTEIN FROM SPINACIA OLERACEA

Abstract A single-stranded ribosome inactivating protein SoRIP1 from Spinacia oleracea (Spinach) is a 30 kDa protein. It is a multipurpose protein with versatile biological activities. This paper defines cloning and expression of SoRIP1 in bacteria to study its versatile biological properties and cytotoxic potential.
PAKEEZA ISMAIL, Dr. ALEENA SUMRIN
openaire   +1 more source

A Simplified Procedure for the Purification of Trichosanthin (A Type 1 Ribosome Inactivating Protein) fromTrichosanthes kirilowiiRoot Tubers

Protein Expression and Purification, 1996
A one step rapid and simple purification procedure for trichosanthin, a type 1 ribosome inactivating protein, from root tubers of Trichosanthes kirilowii has been developed using cation-exchange perfusion chromatography. The identity of the protein has been confirmed by its size, immunoreactivity, and sequence information.
N, Bhatia, K A, McDonald, A P, Jackman, A M, Dandekar   +3 more
openaire   +2 more sources

Crystal structures of a type-1 ribosome inactivating protein from Momordica balsamina in the bound and unbound states

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2012
The ribosome inactivating proteins (RIPs) of type 1 are plant toxins that eliminate adenine base selectively from the single stranded loop of rRNA. We report six crystal structures, type 1 RIP from Momordica balsamina (A), three in complexed states with ribose (B), guanine (C) and adenine (D) and two structures of MbRIP-1 when crystallized with ...
Gajraj Singh, Kushwaha, Nisha, Pandey, Mau, Sinha, S Baskar, Singh, Punit, Kaur, Sujata, Sharma, Tej P, Singh   +6 more
openaire   +2 more sources

Enhanced cytotoxic activity of a bifunctional chimeric protein containing a type 1 ribosome-inactivating protein and a serine protease inhibitor

Biochimie, 2012
Both ribosome-inactivating proteins (RIPs) and plant proteinase inhibitors, belong to protein families known to regulate cellular homeostasis and likely involved in plant defense. Nevertheless the interest in these protein classes is due to their potential use for the treatment of several important human diseases such as cancer.
Rachele Tamburino, PIZZO, ELIODORO, SARCINELLI, CARMEN, Elia Poerio, Francesca Tedeschi, Anna G. Ficca, Augusto Parente, Antimo Di Maro   +7 more
openaire   +5 more sources

Isolation and characterization of four type-1 ribosome-inactivating proteins, with polynucleotide:adenosine glycosidase activity, from leaves of Phytolacca dioica L.

Planta, 1999
A. Di Maro, P. Valbonesi, A. Bolognesi, F. Stirpe, P. De Luca, G. Siniscalco Gigliano, L. Gaudio, P. Delli Bovi, P. Ferranti, A. Malorni, A. Parente   +10 more
semanticscholar   +1 more source

Anti‐CD30 (BER‐H2) immunotoxins containing the type‐1 ribosome‐inactivating proteins momordin and PAP‐S (pokeweed antiviral protein from seeds) display powerful antitumour activity against CD30+ tumour cells in vitro and in SCID mice

British Journal of Haematology, 1996
A. Terenzi, A. Bolognesi, L. Pasqualucci, L. Flenghi, S. Pileri, H. Stein, M. Kadin, B. Bigerna, L. Polito, P. Tazzari, M. Martelli, F. Stirpe, B. Falini   +12 more
semanticscholar   +1 more source

An overview of real‐world data sources for oncology and considerations for research

Ca-A Cancer Journal for Clinicians, 2022
Lynne Penberthy, Donna R Rivera, Jennifer L Lund, Msph   +2 more
exaly