Results 211 to 220 of about 43,196 (233)

Ribosome Profiling and RNA Sequencing Reveal Translation and Transcription Regulation under Acute Heat Stress in Rainbow Trout (Oncorhynchus mykiss, Walbaum, 1792) Liver. [PDF]

Int J Mol Sci
Zhao G, Liu Z, Quan J, Lu J, Li L, Pan Y.   +5 more
europepmc   +1 more source

Improved super-resolution ribosome profiling reveals prevalent translation of upstream ORFs and small ORFs in Arabidopsis. [PDF]

Plant Cell
Wu HL, Ai Q, Teixeira RT, Nguyen PHT, Song G, Montes C, Elmore JM, Walley JW, Hsu PY.   +8 more
europepmc   +1 more source

Anin vitroassay of MCTS1-DENR-dependent re-initiation and ribosome profiling uncover the activity of MCTS2 and distinct function of eIF2D

Meurs R, De Matos M, Bothe A, Guex N, Weber T, Teleman AA, Ban N, Gatfield D.   +7 more
europepmc   +1 more source

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Ribosome Profiling of Plants

2023
Translation is a key step in control of gene expression, yet most analyses of global responses to a stimulus focus on transcription and the transcriptome. For RNA viruses in particular, which have no DNA-templated transcriptional control, control of viral and host translation is crucial.
Jahanara, Sonia, Pulkit, Kanodia, Zachary, Lozier, W Allen, Miller   +3 more
openaire   +2 more sources

Purification of Ribosome-Nascent-Chain Complex for Ribosome Profiling and Selective Ribosome Profiling

2022
Selective Ribosome Profiling (SeRP) is an emerging methodology, developed to capture cotranslational interactions in vivo. To date, SeRP is the only method that can directly capture, in near-codon resolution, ribosomes in action. Thus, SeRP allows us to study the mechanisms of protein synthesis and the network of protein-protein interactions that are ...
Hagit, Bar-Yosef, Johannes, Venezian, Kevin, Klann, Ayala, Shiber   +3 more
openaire   +2 more sources

Analysis of Ribosome Profiling Data

2022
Ribosome profiling methods are based on high-throughput sequencing of ribosome-protected mRNA footprints and allow to study in detail translational changes. Bioinformatic and statistical tools are necessary to analyze sequencing data. Here, we describe our developed methods for a fast and reliable quality control of ribosome profiling data, to ...
Carine Legrand, Khanh Dao Duc, Francesca Tuorto   +2 more
openaire   +2 more sources

Exploring Ribosome-Positioning on Translating Transcripts with Ribosome Profiling

2021
The emergence of ribosome profiling as a tool for measuring the translatome has provided researchers with valuable insights into the post-transcriptional regulation of gene expression. Despite the biological insights and technical improvements made since the technique was initially described by Ingolia et al. (Science 324(5924):218-223, 2009), ribosome
Alexander L, Cope, Sangeevan, Vellappan, John S, Favate, Kyle S, Skalenko, Srujana S, Yadavalli, Premal, Shah   +5 more
openaire   +2 more sources

Ribosome Profiling in Maize

2017
Ribosome profiling (also known as Ribo-seq) provides a genome-wide, high-resolution, and quantitative accounting of mRNA segments that are occupied by ribosomes in vivo. The method has been used to address numerous questions in bacteria, yeast, and metazoa, but its application to questions in plant biology is just beginning.
Prakitchai, Chotewutmontri, Nicholas, Stiffler, Kenneth P, Watkins, Alice, Barkan   +3 more
openaire   +2 more sources

Tissue-Specific Ribosome Profiling in Drosophila

2021
Robust mechanisms exist that serve to dynamically regulate the translation of mRNA into proteins across heterogeneous tissues. These processes ensure timely generation of proteins in quantities that scale with the demands of specific cell types.
Xun, Chen, Dion, Dickman
openaire   +2 more sources