Results 11 to 20 of about 12,203 (191)
Site-Selective Ligand Selection by Mutational Profiling for Covalent RNA Targeting. [PDF]
Angew Chem Int Ed EnglUsing a mutational profiling approach an RNA‐targeted covalent probe was developed that is highly selective for the FMN riboswitch. The covalency was further supported by mass spectrometry experiments. The covalent probe selectively modifies the riboswitch at low micromolar concentrations within 10 min and remains functional when applied to an entire ...
Yesley P +3 more
europepmc +3 more sources
Current Biology, 2023 Riboswitches are structured noncoding RNA domains that are typically found embedded in messenger RNAs, where they sense specific target molecules or elemental ions and regulate gene expression. These RNAs thus serve as genetic switches that can activate or repress gene expression in response to changing levels of their target ligand. To many observers,
Salvail, Hubert, Breaker, Ronald R.
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Metabolic Origin, Role and Fate of the Denaturant Guanidine. [PDF]
Microb BiotechnolThe origin of metabolic guanidine is largely a mystery. We suggest it is created when guanine‐containing nucleotides are oxidised by molecular oxygen instead of being broken down into urea as purines normally would. Guanidine may act as a signal to help cells control the level of reactive oxygen species.
Danchin A +3 more
europepmc +2 more sources
Computational study of unfolding and regulation mechanism of preQ1 riboswitches. [PDF]
PLoS ONE, 2012 Riboswitches are novel RNA regulatory elements. Each riboswitch molecule consists of two domains: aptamer and express platform. The three-dimensional (3D) structure of the aptamer domain, depending on ligand binding or not, controls that of the express ...
Zhou Gong +3 more
doaj +1 more source
A variant of guanidine-IV riboswitches exhibits evidence of a distinct ligand specificity
RNA Biology, 2023 Riboswitches are regulatory RNAs that specifically bind a small molecule or ion. Like metabolite-binding proteins, riboswitches can evolve new ligand specificities, and some examples of this phenomenon have been validated.
Felina Lenkeit +5 more
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RSC Chemical Biology, 2021 Synthetic riboswitches can be used as chemical gene switches in cell-free protein synthesis systems. We provide a current perspective on the state of cell-free riboswitch technologies and their future directions.
Takeshi Tabuchi, Yohei Yokobayashi
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Novel riboswitch ligand analogs as selective inhibitors of guanine-related metabolic pathways. [PDF]
PLoS Pathogens, 2010 Riboswitches are regulatory elements modulating gene expression in response to specific metabolite binding. It has been recently reported that riboswitch agonists may exhibit antimicrobial properties by binding to the riboswitch domain.
Jérôme Mulhbacher +5 more
doaj +1 more source
mBio, 2017 In many bacterial species, the glycine riboswitch is composed of two homologous ligand-binding domains (aptamers) that each bind glycine and act together to regulate the expression of glycine metabolic and transport genes.
Arianne M. Babina +2 more
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Communications Biology, 2022 Molecular dynamic simulations are employed to assess the influence of metal ions on riboswitch structure and dynamics, suggesting a conformational control of riboswitch aptamers by metal ions before ligand binding.
Lei Bao, Wen-Bin Kang, Yi Xiao
doaj +1 more source
Reconsidering the czcD (NiCo) Riboswitch as an Iron Riboswitch
ACS Bio & Med Chem Au, 2022 Recent work has proposed a new mechanism of bacterial iron regulation: riboswitches that undergo a conformational change in response to FeII. The czcD (NiCo) riboswitch was initially proposed to be specific for NiII and CoII, but we recently showed via a czcD-based fluorescent sensor that FeII is also a plausible physiological ligand for this ...
Jiansong Xu, Joseph A. Cotruvo
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