Results 61 to 70 of about 12,203 (191)

Evolution of Prime Editing: Enhancing Efficiency and Expanding Capacity

Advanced Science, EarlyView.
Most rare diseases are caused by genetic mutations. Prime editing (PE) has emerged as a versatile tool capable of inducing diverse mutations without generating DNA double‐strand breaks. Despite its significant clinical potential, PE faces limitations in terms of efficiency and scalability.
Jihyeon Yu, Ju‐Chan Park, Heesoo Uhm, Yong‐Woo Kim, Hyeon Woo Im, Sangsu Bae   +5 more
wiley   +1 more source

Programming Next‐Generation Synthetic Biosensors by Genetic Circuit Design

Advanced Science, EarlyView.
Synthetic biology enables genetic circuit‐based biosensing to detect diverse targets, process signals, and transduce them into readable outputs or intracellular regulatory activities. However, field deployment and real‐world application of such synthetic biosensors face considerable challenges in sensitivity, specificity, speed, stability, and ...
Yuanli Gao, Cheng Huang, Jiaxuan Deng, Lei Wang, Baojun Wang   +4 more
wiley   +1 more source

Metal-ion binding and metal-ion induced folding of the adenine-sensing riboswitch aptamer domain [PDF]

, 2010
Divalent cations are important in the folding and stabilization of complex RNA structures. The adenine-sensing riboswitch controls the expression of mRNAs for proteins involved in purine metabolism by directly sensing intracellular adenine levels ...
Noeske, Jonas, Schwalbe, Harald (Prof. Dr.), Wöhnert, Jens   +2 more
core  

Purine sensing by riboswitches [PDF]

Biology of the Cell, 2008
Structured mRNA elements called riboswitches control gene expression by binding to small metabolites. Over a dozen riboswitch classes have been characterized that target a broad range of molecules and vary widely in size and secondary structure. Four of the known riboswitch classes recognize purines or modified purines.
Jane N, Kim, Ronald R, Breaker
openaire   +2 more sources

Recent Advances in Chemical Probing Strategies for RNA Structure Determination in Vivo

Chemistry – A European Journal, EarlyView.
This Review illustrates how structural probes combined with advanced sequencing technologies are revolutionizing our ability to map RNA structures and interactions within living cells. It highlights the development of innovative strategies tailored to probe RNA structures in particular biological contexts.
Maryana Yarshova, Jieyu Zhao, Chun Kit Kwok   +2 more
wiley   +1 more source

Dynamic energy landscapes of riboswitches help interpret conformational rearrangements and function. [PDF]

PLoS Computational Biology, 2012
Riboswitches are RNAs that modulate gene expression by ligand-induced conformational changes. However, the way in which sequence dictates alternative folding pathways of gene regulation remains unclear.
Giulio Quarta, Ken Sin, Tamar Schlick
doaj   +1 more source

A spermidine riboswitch class in bacteria exploits a close variant of an aptamer for the enzyme cofactor S-adenosylmethionine

Cell Reports, 2023
Summary: Natural polyamines such as spermidine and spermine cations have characteristics that make them highly likely to be sensed by riboswitches, such as their general affinity to polyanionic RNA and their broad contributions to cell physiology ...
Hubert Salvail, Aparaajita Balaji, Adam Roth, Ronald R. Breaker   +3 more
doaj   +1 more source

The return of metabolism: biochemistry and physiology of glycolysis

Biological Reviews, EarlyView.
ABSTRACT Glycolysis is a fundamental metabolic pathway central to the bioenergetics and physiology of virtually all living organisms. In this comprehensive review, we explore the intricate biochemical principles and evolutionary origins of glycolytic pathways, from the classical Embden–Meyerhof–Parnas (EMP) pathway in humans to various prokaryotic and ...
Nana‐Maria Grüning, Federica Agostini, Camila Caldana, Johannes Hartl, Matthias Heinemann, Markus A. Keller, Jan Lukas Krüsemann, Costanza Lamperti, Carole L. Linster, Steffen N. Lindner, Julia Muenzner, Jens Nielsen, Zoran Nikoloski, Bettina Siebers, Jacky L. Snoep, Hezi Tenenboim, Bas Teusink, Spencer J. Williams, Mirjam M. C. Wamelink, Markus Ralser   +19 more
wiley   +1 more source

Aminoglycoside riboswitch control of the expression of integron associated aminoglycoside resistance adenyltransferases

Virulence, 2020
The proliferation of antibiotic resistance has its origins in horizontal gene transfer. The class 1 integrons mediate gene transfer by assimilating antibiotic-resistance genes through site-specific recombination.
Jun Zhang, Getong Liu, Xuhui Zhang, Yaowen Chang, Shasha Wang, Weizhi He, Wenxia Sun, Dongrong Chen, Alastair I.H. Murchie   +8 more
doaj   +1 more source

Regulation of the putative ykkCD riboswitch by tetracycline and related antibiotics in Bacillus subtilis [PDF]

, 2013
Multi-drug resistance among bacterial pathogens can be mediated by a number of mechanisms, including multidrug efflux pumps. One such pump in Bacillus spp. is ykkCD, a heterodimer of the SMR family consisting of C and D subunits.
Frecker, Nicholas L.
core   +2 more sources