Results 101 to 110 of about 43,119 (293)
How do metal ions direct ribozyme folding?
bioRxiv, 2015 Ribozymes, which carry out phosphoryl transfer reactions, often require Mg2+ ions for catalytic activity. The correct folding of the active site and ribozyme tertiary structure is also regulated by metal ions in a manner which is not fully understood ...
N. Denesyuk, D. Thirumalai
semanticscholar +1 more source
Virology Journal, 2010 Background Hepatitis delta virus (HDV) ribozyme is an attractive molecular tool that can specifically recognize and catalyze the self-cleavage of the viral RNA phosphodiester backbone.
Chen Long-Hua +4 more
doaj +1 more source
Plant Biotechnology Journal, EarlyView.Summary A critical step in generating gene‐edited plants is the removal of CRISPR‐related transgenes from T0 plants and their progenies, a process that is generally time‐consuming and labour‐intensive. We previously reported a Transgene Killer CRISPR (TKC) technology that enables self‐elimination of transgenes after the targeted gene has been edited ...
Min Zhu +13 more
wiley +1 more source
Quantitation of In Vitro Activity of Synthetic Trans-Acting Ribozymes Using HPLC
BioTechniques, 1997 The cleavage activity of synthetic ribozymes needs to be characterized by reliable and rapid methods. A chromatographic method to simultaneously quantitate the amounts of substrate, cleavage fragments and ribozyme is described.
L. Citti +4 more
doaj +1 more source
Rescue of Synthetic Genomic RNA Analogs of Rabies Virus by Plasmid-Encoded Proteins [PDF]
, 1994 Proteins eolirely expressed from cDNA wen used to rescue synthetic RNA genome analogs into infectious defective particles or rabies virus (RV). Synthetic negative-stranded RNAs coßtalning 3' · and S'-terminal RV sequences and tnlßscriptional ...
Conzelmann, Karl-Klaus, Schnell, M.-J.
core +3 more sources
Factors determining chromosomal localization of transposable elements in plants
Plant Biology, EarlyView.We review transposon chromosomal niches, and the functional consequences of their specific chromosomal localization in plants. Abstract Transposable elements (TEs) constitute a significant part of plant genomes and shape their genomic landscape. While some TEs are ubiquitously dispersed, other elements specifically occupy discrete genomic loci.
E. Kejnovsky +3 more
wiley +1 more source
Crystal Structure of the VS ribozyme
Nature Chemical Biology, 2015 Varkud Satellite (VS) ribozyme mediates rolling circle replication of a plasmid found in the Neurospora mitochondria. We report crystal structures of this ribozyme at 3.1Å resolution, revealing an intertwined dimer formed by an exchange of substrate ...
N. Suslov +6 more
semanticscholar +1 more source
BMC Research Notes, 2008 Background Hepatitis delta virus (HDV) is a satellite virus of hepatitis B. During viral replication the 1700-nucleotide-long genomic RNA and its complement, the antigenomic RNA, undergo self-cleavage catalyzed by internal ribozyme motifs that are ...
Dobrynina Nadezhda +4 more
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Rethinking “Prebiotic Chemistry”
Perspectives of Earth and Space Scientists, Volume 6, Issue 1, December 2025.Abstract In origins‐of‐life research, the term “prebiotic chemistry” is commonly used to describe processes thought to be related to or required for the emergence of life. However, it suffers from several potential drawbacks that have caused the present group of authors to rethink their usage of the term.
Michael L. Wong +2 more
wiley +1 more source
Ribozyme Mediated gRNA Generation for In Vitro and In Vivo CRISPR/Cas9 Mutagenesis
PLoS ONE, 2016 CRISPR/Cas9 is now regularly used for targeted mutagenesis in a wide variety of systems. Here we report the use of ribozymes for the generation of gRNAs both in vitro and in zebrafish embryos.
R. Lee, A. Ng, P. Ingham
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