Results 81 to 90 of about 18,960 (170)
A hammerhead ribozyme cleaves its target RNA during RNA preparation
A chemically modified hammerhead ribozyme was designed to cleave the tax RNA of human T cell leukemia virus type I. This ribozyme was exogenously delivered to tax-transformed fibroblasts by DOTAP-mediated transfection.
Xu X, Nerenberg M, Heidenreich O
core +2 more sources
The structure and catalytic mechanism of a pseudoknot-containing hammerhead ribozyme
Nature CommunicationsWe have determined the crystal structure of a pseudoknot (PK)-containing hammerhead ribozyme that closely resembles the pistol ribozyme, with essentially identical secondary structure and connectivity. The activity is more sensitive to deletion of the G8
Xuelin Zhan +6 more
doaj +1 more source
Molecular studies on plant glycerol-s-phosphate acyltransferases [PDF]
, 2000 The main objective of this research is to advance our understanding of the biochemical properties and the structure-function relationships of the chloroplast glycerol-3-phosphate acyltransferases in plants.
Kroon, Johannes Theodorus Maria
core
Selective Inactivation of Functional RNAs by Ribozyme-Catalyzed Covalent Modification
, 2016 The diverse functions of RNA provide numerous opportunities for programming biological circuits. We describe a new strategy that uses ribozyme K28min to covalently tag a specific nucleobase within an RNA or DNA target strand to regulate and selectively ...
Melissa P. Lokugamage (3372143) +5 more
core +1 more source
An Alternative Ribozyme for the RNA Hypercycle [PDF]
, 2013 In the quest to forward understanding of the prebiotic world (specifically, the RNA World Hypothesis), one of the most interesting paradigms to consider is the potential for cooperative networks that may have driven molecular evolution forward.
Elkhal, Ishak
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Comparative analysis of Helicase 3’ UTR with Hepatitis Delta Virus (HDV) ribozyme (PDB Id: 4PR6).
, 2016 (a) Tertiary structure of HDV Ribozyme (4PR6). (b) Sequence alignment of Helicase 3’ UTR and HDV ribozyme. (c) Structural superimposition of HDV ribozyme (4PR6) and Helicase ribozyme where HDV ribozyme is represented in red and Helicase 3’ UTR in blue ...
Pruthvi Raj Bejugam (282134) +1 more
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Ribozymes based self-cleavage sgRNA and its application in gene editing
四川大学学报. 自然科学版, 2019 Currently, sgRNAs are usually transcribed by type Ⅲ promoters in CRISPR/Cas9 system, which makes it difficult to achieve tissuespecific and timespecific expression. Cytotoxicity is an additional concern for constitutive transcription of small RNAs.
Shan Ce, Li Zhong-Han
doaj
Folding and activity of the hammerhead ribozyme.
, 2002 The hammerhead is the smallest of the nucleolytic ribozymes, that undergo backbone cleavage by a transesterification reaction in the presence of magnesium ions.
Lilley, David M. J., Hammann, Christian
core
Kinetic Mechanism of the Hairpin Ribozyme [PDF]
, 1997 To investigate the relationship between RNA folding and ribozyme catalysis, we have carried out a detailed kinetic analysis of four structural derivatives of the hairpin ribozyme. Optimal and suboptimal (wild-type) substrate sequences were studied in
Banerjee, Aloke Raj +2 more
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Ribozyme-catalysed RNA synthesis using triplet building blocks
eLife, 2018 RNA-catalyzed RNA replication is widely believed to have supported a primordial biology. However, RNA catalysis is dependent upon RNA folding, and this yields structures that can block replication of such RNAs.
James Attwater +4 more
doaj +1 more source