Results 81 to 90 of about 5,544 (121)
A structural analysis of
BMC Bioinformatics, 2007 Background Ribozymes are small catalytic RNAs that possess the dual functions of sequence-specific RNA recognition and site-specific cleavage. Trans-cleaving ribozymes can inhibit translation of genes at the messenger RNA (mRNA) level in both eukaryotic ...
Schneider Erasmus +5 more
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An Alternative Ribozyme for the RNA Hypercycle [PDF]
, 2013 In the quest to forward understanding of the prebiotic world (specifically, the RNA World Hypothesis), one of the most interesting paradigms to consider is the potential for cooperative networks that may have driven molecular evolution forward.
Elkhal, Ishak
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The structure and catalytic mechanism of a pseudoknot-containing hammerhead ribozyme
Nature CommunicationsWe have determined the crystal structure of a pseudoknot (PK)-containing hammerhead ribozyme that closely resembles the pistol ribozyme, with essentially identical secondary structure and connectivity. The activity is more sensitive to deletion of the G8
Xuelin Zhan +6 more
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Ribozyme mediated destruction of RNA in vivo
, 1989 Previous studies have demonstrated that high ribozyme to substrate ratios are required for ribozyme inhibitory function in nuclear extracts. To obtain high intracellular levels of ribozymes, tRNA genes, known to be highly expressed in most tissues, have ...
Cotten, M., Birnstiel, M.L.
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Ribozymes based self-cleavage sgRNA and its application in gene editing
四川大学学报. 自然科学版, 2019 Currently, sgRNAs are usually transcribed by type Ⅲ promoters in CRISPR/Cas9 system, which makes it difficult to achieve tissuespecific and timespecific expression. Cytotoxicity is an additional concern for constitutive transcription of small RNAs.
Shan Ce, Li Zhong-Han
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Folding and catalysis by the VS ribozyme
, 2002 The VS ribozyme is a 154 nt self-cleaving RNA molecule that can be divided into a trans-acting five-helix ribozyme and stem-loop substrate. The structure of the ribozyme is organised by two three-way helical junctions, the structure of which has been ...
Norman, David G. +2 more
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Kinetic Mechanism of the Hairpin Ribozyme [PDF]
, 1997 To investigate the relationship between RNA folding and ribozyme catalysis, we have carried out a detailed kinetic analysis of four structural derivatives of the hairpin ribozyme. Optimal and suboptimal (wild-type) substrate sequences were studied in
Banerjee, Aloke Raj +2 more
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Short-term sequence evolution and vertical inheritance of the
BMC Evolutionary Biology, 2006 Background Ribosomal DNA of several species of the free-living Naegleria amoeba harbors an optional group I intron within the nuclear small subunit ribosomal RNA gene.
De Jonckheere Johan F +3 more
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Intracellular ribozyme applications
, 2002 The exquisite target selectivity of trans -acting ribozymes has fostered their use as potential therapeutic agents and tools for down-regulating cellular transcripts. In living cells, free diffusion of RNAs is extremely limited, if it exists at all. Thus,
Michienzi, A +7 more
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The ubiquitous hammerhead ribozyme
, 2012 The hammerhead ribozyme is a small catalytic RNA motif capable of endonucleolytic (self-) cleavage. It is composed of a catalytic core of conserved nucleotides flanked by three helices, two of which form essential tertiary interactions for fast self ...
De La Peña, Marcos +4 more
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