Results 71 to 80 of about 27,058 (211)

The 10–23 DNAzyme in Biosensing and Diagnostics: Applications, Challenges, and Future Directions

Angewandte Chemie, Volume 138, Issue 18, 27 April 2026.
This review focuses on the 10–23 DNAzyme in diagnostics, spanning unregulated and regulated target‐recognition modes, and their functionalization across colorimetric, fluorescent, electrochemical, electrochemiluminescent, and intracellular biosensing strategies.
Connor Nurmi, Jake Brill, Sanne Roumans, Amal Mathai, John D. Brennan, Yingfu Li   +5 more
wiley   +2 more sources

Evidence for the involvement of G_i2 in activation of extracellular signal-regulated kinases in hepatocytes

BMC Cell Biology, 2001
Background Activation of the extracellular signal-regulated kinases ERK1 and ERK2 in hepatocytes by prostaglandin (PG)F2α was recently found to be inhibited by pertussis toxin (PTX) suggesting a role for Gi proteins. Results Targeting the Gi2α expression
Sioud Mouldy, Christoffersen Thoralf, Melien Øyvind   +2 more
doaj   +1 more source

Chemical Syntheses of 3′ and 5′ Phosphorylated Oligonucleotides: An Overview

European Journal of Organic Chemistry, EarlyView.
This review provides an overview of the various chemical methods employed in the synthesis of oligonucleotides bearing a phosphate group at their 3′ or 5′ end. Notably, it presents modified phosphoramidites and supports developed for this purpose. Oligonucleotides (ON) with a phosphate monoester group at their 3′ or 5′ end have applications in many ...
Rémy Lartia
wiley   +1 more source

Quantitation of In Vitro Activity of Synthetic Trans-Acting Ribozymes Using HPLC

BioTechniques, 1997
The cleavage activity of synthetic ribozymes needs to be characterized by reliable and rapid methods. A chromatographic method to simultaneously quantitate the amounts of substrate, cleavage fragments and ribozyme is described.
L. Citti, L. Boldrini, S. Nevischi, L. Mariani, G. Rainaldi   +4 more
doaj   +1 more source

The effects of anti-Fas ribozyme on T lymphocyte apoptosis in mice model with chronic obstructive pulmonary disease [PDF]

Iranian Journal of Basic Medical Sciences, 2017
Objective(s): In this study, we aimed to investigate the effects of anti-Fas ribozyme on the apoptosis of T lymphocytes (T cells) in mice model with chronic obstructive pulmonary disease (COPD).
Song-Ming Zhuo, Si-Cong Li, Yong-Qun Lin, Hai-Bin Yu, Na Li   +4 more
doaj   +1 more source

Enhancing CRISPR‐Cas12a base editing in plants with LbCas12a variants and introns

Journal of Integrative Plant Biology, EarlyView.
Intron optimization of LbCas12a‐RRV improves cytosine and adenine base editing efficiency in plants, supports multiplexed and double‐strand break‐free genome modification, and expands precise genome engineering tools for crop breeding and plant functional research. ABSTRACT Cytosine base editors (CBEs) and adenine base editors (ABEs) are powerful tools
Yanhao Cheng, Gen Li, Man Zhou, Rushil Mandlik, Doris Wang, Yiping Qi   +5 more
wiley   +1 more source

The Role of Chemistry Across Disciplines From Humanities to Life Sciences in Understanding Complexity and Emergence

Angewandte Chemie, Volume 138, Issue 17, 20 April 2026.
This study explores the origins of life by linking prebiotic chemistry, the emergence of information‐carrying molecules such as RNA and proteins, and philosophical questions about consciousness. The study emphasizes the role of molecular evolution in the Central Dogma and provides insights into the chemical origins of biology and the basis of life's ...
Harald Schwalbe, Josef Wachtveitl, Alexander Heckel, Florian Buhr, Sabrina Toews, Thomas M. Schimmer   +5 more
wiley   +2 more sources

Efficient inhibition of hepatitis B virus replication by hepatitis delta virus ribozymes delivered by targeting retrovirus

Virology Journal, 2010
Background Hepatitis delta virus (HDV) ribozyme is an attractive molecular tool that can specifically recognize and catalyze the self-cleavage of the viral RNA phosphodiester backbone.
Chen Long-Hua, Qi Peng, Lu Yan-Qin, Wang Chuan-Xi, Han Jin-Xiang   +4 more
doaj   +1 more source

Enhanced RNA knockdown efficiency with engineered fusion guide RNAs that function with both CRISPR-CasRx and hammerhead ribozyme

Genome Biology, 2023
Background CRISPR-Cas13 is a newly emerging RNA knockdown technology that is comparable to RNAi. Among all members of Cas13, CasRx degrades RNA in human cells with high precision and effectiveness.
Yonghao Zhan, Congcong Cao, Aolin Li, Hongbing Mei, Yuchen Liu   +4 more
doaj   +1 more source

RNase footprinting demonstrates antigenomic hepatitis delta virus ribozyme structural rearrangement as a result of self-cleavage reaction

BMC Research Notes, 2008
Background Hepatitis delta virus (HDV) is a satellite virus of hepatitis B. During viral replication the 1700-nucleotide-long genomic RNA and its complement, the antigenomic RNA, undergo self-cleavage catalyzed by internal ribozyme motifs that are ...
Dobrynina Nadezhda, Belyanko Tatyana, Alekseenkova Victoria, Savochkina Larissa, Beabealashvilli Robert   +4 more
doaj   +1 more source