Results 71 to 80 of about 18,960 (170)
The global structure of the VS ribozyme
, 2002 The VS ribozyme comprises five helical segments (II–VI) in a formal H shape, organized by two three-way junctions. It interacts with its stem–loop substrate (I) by tertiary interactions. We have determined the global shape of the 3–4–5 junction (relating
Norman, David G. +2 more
core +1 more source
A recombinase-activated ribozyme to knock down endogenous gene expression in zebrafish.
PLoS GeneticsPrecise regulation of gene expression is essential to understand a wide range of biological processes. Control over gene expression can be achieved using site-directed recombinases and endonucleases whose efficiency is variable and dependent on the ...
Thomas Juan +6 more
doaj +1 more source
Fully Reversible Regulation of 8‐17 DNAzyme Activity Using Nucleoside‐Based Diarylethenes
Chemistry – A European Journal, Volume 32, Issue 35, 19 September 2026.The site‐specific incorporation of nucleosidic diarylethenes into the 8–17 deoxyribozyme (DNAzyme) identified position 2.1 as optimal. Compound dU‐PhtBu enabled efficient light‐dependent control of catalytic activity, while maintaining thermal stability and reversibility.
Jörn Bargstedt +3 more
wiley +1 more source
Quantitation of In Vitro Activity of Synthetic Trans-Acting Ribozymes Using HPLC
BioTechniques, 1997 The cleavage activity of synthetic ribozymes needs to be characterized by reliable and rapid methods. A chromatographic method to simultaneously quantitate the amounts of substrate, cleavage fragments and ribozyme is described.
L. Citti +4 more
doaj +1 more source
Ovarian Cancer Diagnosis and Chemoresistance Prediction Model Based on cfRNA Molecular Signature
Advanced Science, Volume 13, Issue 52, 18 September 2026.A deep learning model analyzes cfRNA profiles extracted from the blood of OVCA patients. This innovative approach distinguishes OVCA from healthy controls with high accuracy. Crucially, it reliably predicts patient response to chemotherapy (sensitive versus resistant subgroups).
Qinhao Guo +14 more
wiley +1 more source
A structural analysis of
BMC Bioinformatics, 2007 Background Ribozymes are small catalytic RNAs that possess the dual functions of sequence-specific RNA recognition and site-specific cleavage. Trans-cleaving ribozymes can inhibit translation of genes at the messenger RNA (mRNA) level in both eukaryotic ...
Schneider Erasmus +5 more
doaj +1 more source
Folding and catalysis by the VS ribozyme
, 2002 The VS ribozyme is a 154 nt self-cleaving RNA molecule that can be divided into a trans-acting five-helix ribozyme and stem-loop substrate. The structure of the ribozyme is organised by two three-way helical junctions, the structure of which has been ...
Norman, David G. +2 more
core +1 more source
BMC Research Notes, 2008 Background Hepatitis delta virus (HDV) is a satellite virus of hepatitis B. During viral replication the 1700-nucleotide-long genomic RNA and its complement, the antigenomic RNA, undergo self-cleavage catalyzed by internal ribozyme motifs that are ...
Dobrynina Nadezhda +4 more
doaj +1 more source
Ribozyme mediated destruction of RNA in vivo
, 1989 Previous studies have demonstrated that high ribozyme to substrate ratios are required for ribozyme inhibitory function in nuclear extracts. To obtain high intracellular levels of ribozymes, tRNA genes, known to be highly expressed in most tissues, have ...
Cotten, M., Birnstiel, M.L.
core +1 more source
Virus‐Induced Genome Editing in the Parasitic Plant Phtheirospermum japonicum
Plant, Cell &Environment, EarlyView.
Hannes Ruwe +3 more
wiley +1 more source