Results 71 to 80 of about 5,544 (121)
Chemical Biology &Drug Design, Volume 108, Issue 2, August 2026.Chemical and structural engineering strategies enhance gRNA stability, activity, specificity, and translational potential for precision genome editing. This review summarizes recent advances, design principles, and emerging clinical applications of engineered gRNAs.
Masaki Kawamata +2 more
wiley +1 more source
Plant Biotechnology Journal, Volume 24, Issue 8, Page 4933-4947, August 2026.ABSTRACT Plant viruses are recognized as rapid and effective vectors to deliver CRISPR‐Cas reaction components into plants, a strategy termed virus‐induced gene editing (VIGE). However, VIGE is limited by the host range of the viral vectors. Development of new viral vectors to target a broad range of plant species will potentially enable the delivery ...
Fernando Merwaiss +3 more
wiley +1 more source
A Selected Ribozyme Catalyzing Diverse Dipeptide Synthesis [PDF]
, 2002 The sequence of events by which protein, RNA, and DNA emerged during early biological evolution is one of the most profound questions regarding the origin of life.
Gottlieb, Robert L +3 more
core +1 more source
How physiology solves the gene‐centric impasse
Experimental Physiology, Volume 111, Issue 8, Page 3546-3556, 1 August 2026.Abstract The criteria for distinguishing between association and causation in studies of genes and function are clarified. Genomic association scores alone cannot satisfy those criteria, which is why treatment of common diseases has not benefitted from the cures expected when the Human Genome Project was launched.
Denis Noble, Reine Bourret
wiley +1 more source
Secreted Nonstructural Protein 3 is a Pathogenic Determinant of Orbivirus
Advanced Science, Volume 13, Issue 42, 28 July 2026.This study uncovers a conserved PIP2‐dependent secretory pathway of orbivirus NS3 that induces vascular leakage. Pharmacological disruption of PIP2‐NS3 interaction significantly reduces viral pathogenicity and provides protective efficacy in murine models, establishing PIP2‐mediated NS3 secretion as both a key virulence determinant and a promising ...
Junyong Guan +11 more
wiley +1 more source
Quantitation of In Vitro Activity of Synthetic Trans-Acting Ribozymes Using HPLC
BioTechniques, 1997 The cleavage activity of synthetic ribozymes needs to be characterized by reliable and rapid methods. A chromatographic method to simultaneously quantitate the amounts of substrate, cleavage fragments and ribozyme is described.
L. Citti +4 more
doaj +1 more source
A recombinase-activated ribozyme to knock down endogenous gene expression in zebrafish.
PLoS GeneticsPrecise regulation of gene expression is essential to understand a wide range of biological processes. Control over gene expression can be achieved using site-directed recombinases and endonucleases whose efficiency is variable and dependent on the ...
Thomas Juan +6 more
doaj +1 more source
Exonuclease Ribozyme Encoded in a Circular Genome: On the Initiation of the RNA World
LifeThe “RNA World” hypothesis posits a reasonable scenario for the origin of life. A question central to this scenario is: which RNA species may have emerged as the first ribozyme, thereby initiating the earliest stage of life?
Minglun Liang +3 more
doaj +1 more source
BMC Research Notes, 2008 Background Hepatitis delta virus (HDV) is a satellite virus of hepatitis B. During viral replication the 1700-nucleotide-long genomic RNA and its complement, the antigenomic RNA, undergo self-cleavage catalyzed by internal ribozyme motifs that are ...
Dobrynina Nadezhda +4 more
doaj +1 more source
Analysis of Self-Cleaving Ribozyme Transition States [PDF]
, 2019 Endonucleolytic ribozymes, non-coding RNA molecules capable of self-scission, have been studied for decades. First, because of their novel functionality, and later because their capacity to serve as paradigmatic model systems of RNA catalysis.
Weissman, Benjamin
core +1 more source