Results 71 to 80 of about 16,956 (222)

Chemical Syntheses of 3′ and 5′ Phosphorylated Oligonucleotides: An Overview

European Journal of Organic Chemistry, Volume 29, Issue 21, 4 June 2026.
This review provides an overview of the various chemical methods employed in the synthesis of oligonucleotides bearing a phosphate group at their 3′ or 5′ end. Notably, it presents modified phosphoramidites and supports developed for this purpose. Oligonucleotides (ON) with a phosphate monoester group at their 3′ or 5′ end have applications in many ...
Rémy Lartia
wiley   +1 more source

Quantitation of In Vitro Activity of Synthetic Trans-Acting Ribozymes Using HPLC

BioTechniques, 1997
The cleavage activity of synthetic ribozymes needs to be characterized by reliable and rapid methods. A chromatographic method to simultaneously quantitate the amounts of substrate, cleavage fragments and ribozyme is described.
L. Citti, L. Boldrini, S. Nevischi, L. Mariani, G. Rainaldi   +4 more
doaj   +1 more source

Enhanced RNA knockdown efficiency with engineered fusion guide RNAs that function with both CRISPR-CasRx and hammerhead ribozyme

Genome Biology, 2023
Background CRISPR-Cas13 is a newly emerging RNA knockdown technology that is comparable to RNAi. Among all members of Cas13, CasRx degrades RNA in human cells with high precision and effectiveness.
Yonghao Zhan, Congcong Cao, Aolin Li, Hongbing Mei, Yuchen Liu   +4 more
doaj   +1 more source

Spontaneous Non‐Catalyzed Molecular Reactions and Interactions in the Human Body: Biomedical Implications

Advanced Science, Volume 13, Issue 36, 29 June 2026.
ABSTRACT The human body functions as a natural reactor for a vast network of chemical and biological reactions and physical interactions among small molecules, proteins, cells, and numerous other components. These reactions/interactions are essential for maintaining normal physiological functions.
Yuhao Cai, Chao Zhao
wiley   +1 more source

RNase footprinting demonstrates antigenomic hepatitis delta virus ribozyme structural rearrangement as a result of self-cleavage reaction

BMC Research Notes, 2008
Background Hepatitis delta virus (HDV) is a satellite virus of hepatitis B. During viral replication the 1700-nucleotide-long genomic RNA and its complement, the antigenomic RNA, undergo self-cleavage catalyzed by internal ribozyme motifs that are ...
Dobrynina Nadezhda, Belyanko Tatyana, Alekseenkova Victoria, Savochkina Larissa, Beabealashvilli Robert   +4 more
doaj   +1 more source

Nucleic Acid Therapeutics for “Undruggable” Cancer Targets: Mechanisms, Challenges, and Prospects

Advanced Science, Volume 13, Issue 36, 29 June 2026.
Nucleic acid therapeutics bypass the structural limitations of conventional drugs by targeting mRNA rather than proteins. This review examines how antisense oligonucleotides, siRNAs, miRNAs, aptamers, and mRNA vaccines intervene against historically undruggable oncoproteins including Ras, MYC, and p53, highlighting mechanistic advances, delivery ...
Feng Xu, Ke Wang, Kaizhong Lu, Tianlun Hou, Yuan Chen, Xian Wang, Hongchuan Jin   +6 more
wiley   +1 more source

In vitro Evolution and Engineering for Improved Ribozyme Polymerase Production

, 2011
According to the "RNA World Hypothesis" the first self replicating molecule formed from the prebiotic chemicals is the ribozyme. A model compartmentalized self replicating system for ribozyme polymerases is designed and used to improve activity,
Muhammad Waheed Akktar, Ayaz Ali Khan, Israr Khan   +2 more
core  

The ubiquitous hammerhead ribozyme

, 2012
The hammerhead ribozyme is a small catalytic RNA motif capable of endonucleolytic (self-) cleavage. It is composed of a catalytic core of conserved nucleotides flanked by three helices, two of which form essential tertiary interactions for fast self ...
De La Peña, Marcos, Perreault, Jonathan, Hammann, Christian, Peña, Marcos de la, Luptak, Andrej   +4 more
core   +1 more source

Ribozyme für die RNA‐katalysierte Methylierung und Markierung von RNA

Angewandte Chemie, Volume 138, Issue 23, 1 June 2026.
Ribozyme sind vielseitige Werkzeuge für die präzise Modifikation von RNA. Das Reaktionsspektrum in‐vitro‐selektierter Ribozyme reicht vom Einbau funktioneller Gruppen und Fluorophoren bis zu natürlichen RNA‐Methylierungen. Dieser Übersichtsartikel präsentiert Cofaktor‐abhängige Ribozyme für die positionsgenaue Markierung und Modifikation von RNA ...
Carolin P. M. Scheitl, Claudia Höbartner   +1 more
wiley   +1 more source

A recombinase-activated ribozyme to knock down endogenous gene expression in zebrafish.

PLoS Genetics
Precise regulation of gene expression is essential to understand a wide range of biological processes. Control over gene expression can be achieved using site-directed recombinases and endonucleases whose efficiency is variable and dependent on the ...
Thomas Juan, Tonatiuh Molina, Lihan Xie, Sofia Papadopoulou, Bárbara Cardoso, Shivam Govind Jha, Didier Y R Stainier   +6 more
doaj   +1 more source