Results 51 to 60 of about 18,960 (170)
Exploration, EarlyView.This review highlights recent advances in engineering messenger RNA (mRNA)‐lipid nanoparticles (LNPs) to cross the ageing blood–brain barrier and target neurodegenerative diseases. It outlines design principles, delivery routes, and translational challenges, charting a roadmap towards clinical application of mRNA‐LNP therapeutics for neurodegenerative ...
Abdel Ali Belaidi +5 more
wiley +1 more source
Inducible knockdown of Plasmodium gene expression using the glmS ribozyme.
PLoS ONE, 2013 Conventional reverse genetic approaches for study of Plasmodium malaria parasite gene function are limited, or not applicable. Hence, new inducible systems are needed. Here we describe a method to control P.
Parichat Prommana +7 more
doaj +1 more source
Schematic of the glmS ribozyme reverse genetic tool.
, 2013 The ribozyme is inserted in the 3′-UTR after the coding region so that it is present in the expressed mRNA. Following addition of the inducer, glucosamine, which binds to the ribozyme, the mRNA self-cleaves resulting in degradation of the mRNA and knock ...
Anthony A. Holder (53358) +7 more
core +1 more source
CPEB3 is associated with human episodic memory
Frontiers in Behavioral Neuroscience, 2009 Cytoplasmic polyadenylation element-binding (CPEB) proteins are crucial for synaptic plasticity and memory in model organisms. A highly conserved, mammalian-specific short intronic sequence within CPEB3 has been identified as a ribozyme with self ...
Christian Vogler +14 more
doaj +1 more source
Molecular Anatomy of the Class I Ligase Ribozyme for Elucidation of the Activity-Generating Unit
Biology, 2023 The class I ligase ribozyme consists of 121 nucleotides and shows a high catalytic rate comparable to that found in natural proteinaceous polymerases. In this study, we aimed to identify the smaller active unit of the class I ligase ribozyme comprising ...
Miho Kasuga +3 more
doaj +1 more source
Nucleobase participation in ribozyme catalysis
, 2005 We constructed a modified form of the VS ribozyme containing an imidazole ring in place of adenine at position 756. The novel ribozyme is active in both cleavage and ligation reactions. The reaction is efficient, although relatively slow. The results are
Araki, Lisa +6 more
core +1 more source
Analysis of Self-Cleaving Ribozyme Transition States [PDF]
, 2019 Endonucleolytic ribozymes, non-coding RNA molecules capable of self-scission, have been studied for decades. First, because of their novel functionality, and later because their capacity to serve as paradigmatic model systems of RNA catalysis.
Weissman, Benjamin
core +1 more source
Use of a Lariat Capping Ribozyme to Study Cap Function In Vivo
, 2021 A lariat cap is a naturally occurring substitute of a conventional mRNA cap and is found in a particular genomic setting in a few eukaryotic microorganisms. It is installed by the lariat capping ribozyme acting in cis.
Tempel, Gregor +4 more
core +1 more source
Small Methods, EarlyView.Combining synthetic cells engineering, cell‐free expression, and optical regulation may enable a new generation of programmable synthetic cell platforms. By providing spatiotemporal control over biomolecule synthesis, activation, transport, release, and communication, light offers a versatile strategy for coordinating proteins, RNAs, peptides, and ...
Gia Bao Leon Hoang, Taniya Chakraborty
wiley +1 more source
RNA-peptide interactions tune the ribozyme activity within coacervate microdroplet dispersions
Nature CommunicationsMembrane-free complex coacervate microdroplets are compelling models for primitive compartmentalisation with the ability to form from biological molecules.
Basusree Ghosh +8 more
doaj +1 more source