Results 51 to 60 of about 27,058 (211)
Beyond seek and destroy: How to generate allelic series using genome editing tools [PDF]
, 2020 Genome editing tools have greatly facilitated the functional analysis of genes of interest by targeted mutagenesis. Many usable genome editing tools, including different site-specific nucleases and editor databases that allow single-nucleotide ...
Bes, Martine +10 more
core +3 more sources
In vitro Evolution and Engineering for Improved Ribozyme Polymerase Production [PDF]
, 2011 According to the "RNA World Hypothesis" the first self replicating molecule formed from the prebiotic chemicals is the ribozyme. A model compartmentalized self replicating system for ribozyme polymerases is designed and used to improve activity,
Ayaz Ali Khan +2 more
core +1 more source
Processing of insect retrotransposons by self-cleaving ribozymes [PDF]
, 2010 We show that several classes of insect non-LTR retrotransposons harbor self-cleaving ribozymes of the HDV family at their 5′ termini. In Drosophila the R2 ribozymes exhibit highly differential in vivo expression and robust in vitro activity ...
Andrej Luptak +3 more
core +1 more source
Selfishness versus functional cooperation in a stochastic protocell model [PDF]
, 2010 How to design an "evolvable" artificial system capable to increase in complexity? Although Darwin's theory of evolution by natural selection obviously offers a firm foundation, little hope of success seems to be expected from the explanatory adequacy of ...
Agresti +56 more
core +2 more sources
Ribozyme für die RNA‐katalysierte Methylierung und Markierung von RNA
Angewandte Chemie, EarlyView.Ribozyme sind vielseitige Werkzeuge für die präzise Modifikation von RNA. Das Reaktionsspektrum in‐vitro‐selektierter Ribozyme reicht vom Einbau funktioneller Gruppen und Fluorophoren bis zu natürlichen RNA‐Methylierungen. Dieser Übersichtsartikel präsentiert Cofaktor‐abhängige Ribozyme für die positionsgenaue Markierung und Modifikation von RNA ...
Carolin P. M. Scheitl +1 more
wiley +1 more source
Plant Methods, 2022 Background Traditional CRISPR/Cas9 systems that rely on U6 or U3 snRNA promoters (RNA polymerase III-dependent promoters) can only achieve constitutive gene editing in plants, hampering the functional analysis of specifically expressed genes.
Jia-Wen Li +9 more
doaj +1 more source
Applied Sciences, 2021 Ribozymes are catalytic RNAs that are attractive platforms for the construction of nanoscale objects with biological functions. We designed a dimeric form of the Tetrahymena group I ribozyme as a unit structure in which two ribozymes were connected in a ...
Junya Akagi +8 more
doaj +1 more source
Stem-cell-based gene therapy for HIV infection. [PDF]
, 2013 Despite the enormous success of combined anti-retroviral therapy, HIV infection is still a lifelong disease and continues to spread rapidly worldwide. There is a pressing need to develop a treatment that will cure HIV infection.
Kitchen, Scott, Zhen, Anjie
core +2 more sources
Alkyltransferase Ribozyme for Site‐Specific N4‐Cytidine Alkylation
Angewandte Chemie, Volume 138, Issue 20, 11 May 2026.CSAR is the first ribozyme that catalyzes direct alkylation of an exocyclic amino group of an RNA nucleobase. The ribozyme was isolated as a self‐labeling catalyst from an in vitro selection library and engineered into a trans‐active ribozyme that catalyzes the transfer of a benzyl group from an O6‐modifed guanine to the N4 amino group of a specific ...
Evgeniia Dorinova +2 more
wiley +2 more sources
Witnessing the structural evolution of an RNA enzyme
eLife, 2021 An RNA polymerase ribozyme that has been the subject of extensive directed evolution efforts has attained the ability to synthesize complex functional RNAs, including a full-length copy of its own evolutionary ancestor.
Xavier Portillo +4 more
doaj +1 more source