Results 51 to 60 of about 16,956 (222)
Plant Methods, 2022 Background Traditional CRISPR/Cas9 systems that rely on U6 or U3 snRNA promoters (RNA polymerase III-dependent promoters) can only achieve constitutive gene editing in plants, hampering the functional analysis of specifically expressed genes.
Jia-Wen Li +9 more
doaj +1 more source
Plant Biotechnology Journal, EarlyView.ABSTRACT Plant viruses are recognized as rapid and effective vectors to deliver CRISPR‐Cas reaction components into plants, a strategy termed virus‐induced gene editing (VIGE). However, VIGE is limited by the host range of the viral vectors. Development of new viral vectors to target a broad range of plant species will potentially enable the delivery ...
Fernando Merwaiss +3 more
wiley +1 more source
Molecular studies on plant glycerol-s-phosphate acyltransferases [PDF]
, 2000 The main objective of this research is to advance our understanding of the biochemical properties and the structure-function relationships of the chloroplast glycerol-3-phosphate acyltransferases in plants.
Kroon, Johannes Theodorus Maria
core
Études d'ingénierie du ribozyme VS de Neurospora [PDF]
, 2015 Les ribozymes sont des ARN catalytiques fréquemment exploités pour le développement d’outils biochimiques et d’agents thérapeutiques. Ils sont particulièrement intéressants pour effectuer l’inactivation de gènes, en permettant la dégradation d’ARNm ou
Lacroix-Labonté, Julie
core +1 more source
Design and Preparation of a Multimeric Self-Cleaving Hammerhead Ribozyme
BioTechniques, 1997 The activity of a ribozyme can be impaired by additional sequences at the 5′ and 3′ termini of the catalytic sequence. To approach this problem, a system was designed that minimizes sequences upstream and downstream from active regions of a hammerhead ...
Juan Ruiz +3 more
doaj +1 more source
Molecular Anatomy of the Class I Ligase Ribozyme for Elucidation of the Activity-Generating Unit
Biology, 2023 The class I ligase ribozyme consists of 121 nucleotides and shows a high catalytic rate comparable to that found in natural proteinaceous polymerases. In this study, we aimed to identify the smaller active unit of the class I ligase ribozyme comprising ...
Miho Kasuga +3 more
doaj +1 more source
How physiology solves the gene‐centric impasse
Experimental Physiology, EarlyView.Abstract The criteria for distinguishing between association and causation in studies of genes and function are clarified. Genomic association scores alone cannot satisfy those criteria, which is why treatment of common diseases has not benefitted from the cures expected when the Human Genome Project was launched.
Denis Noble, Reine Bourret
wiley +1 more source
Inducible knockdown of Plasmodium gene expression using the glmS ribozyme.
PLoS ONE, 2013 Conventional reverse genetic approaches for study of Plasmodium malaria parasite gene function are limited, or not applicable. Hence, new inducible systems are needed. Here we describe a method to control P.
Parichat Prommana +7 more
doaj +1 more source
Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme
Nature Communications, 2020 RNA sequence-structure-function relationships can be studied via assays of variant activity. The authors developed a method to systematically determine ribozyme variant kinetics, using it to map double-mutant interactions and show that cleavage rate ...
Johan O. L. Andreasson +3 more
doaj +1 more source
The effects of anti-Fas ribozyme on T lymphocyte apoptosis in mice model with chronic obstructive pulmonary disease [PDF]
Iranian Journal of Basic Medical Sciences, 2017 Objective(s): In this study, we aimed to investigate the effects of anti-Fas ribozyme on the apoptosis of T lymphocytes (T cells) in mice model with chronic obstructive pulmonary disease (COPD).
Song-Ming Zhuo +4 more
doaj +1 more source