Results 41 to 50 of about 5,544 (121)
Nucleobase participation in ribozyme catalysis
, 2005 We constructed a modified form of the VS ribozyme containing an imidazole ring in place of adenine at position 756. The novel ribozyme is active in both cleavage and ligation reactions. The reaction is efficient, although relatively slow. The results are
Araki, Lisa +6 more
core +1 more source
GROUP I INTRON-DERIVED RIBOZYME REACTIONS [PDF]
, 2005 Group I introns are catalytic RNAs capable of self-splicing out of RNA transcripts. Ribozymes derived from these group I introns are used to explore the molecular recognition properties involved in intron catalysis.
Johnson, Ashley Kirtley
core +1 more source
Witnessing the structural evolution of an RNA enzyme
eLife, 2021 An RNA polymerase ribozyme that has been the subject of extensive directed evolution efforts has attained the ability to synthesize complex functional RNAs, including a full-length copy of its own evolutionary ancestor.
Xavier Portillo +4 more
doaj +1 more source
Fully Reversible Regulation of 8‐17 DNAzyme Activity Using Nucleoside‐Based Diarylethenes
Chemistry – A European Journal, EarlyView.The site‐specific incorporation of nucleosidic diarylethenes into the 8–17 deoxyribozyme (DNAzyme) identified position 2.1 as optimal. Compound dU‐PhtBu enabled efficient light‐dependent control of catalytic activity, while maintaining thermal stability and reversibility.
Jörn Bargstedt +3 more
wiley +1 more source
Split intron-exon system for circular RNA synthesis
Nature CommunicationsRibozyme-based approaches have been extensively employed for in vitro circular RNA (circRNA) synthesis. However, several critical challenges are impeding the progress in this field, including the relatively low ribozyme utilization efficiency, limited ...
Lei Wang +11 more
doaj +1 more source
Chemistry – A European Journal, EarlyView.An electrophilic 5‐isothiocyanatomethyl uridine building block enables modular post‐synthetic RNA labeling with primary amine‐containing probes. Positioning the functionality in the major groove minimizes structural perturbation and allows facile conjugation of sensitive tags such as TEMPO spin labels for paramagnetic relaxation enhancement studies ...
Lisa Ruetz +4 more
wiley +1 more source
Characterization of D135 group II intron ribozyme dimerization [PDF]
, 2013 textGroup II introns are highly structured RNAs that carry out self-splicing reactions. The multiple turnover version of one of these introns, termed the D135 ribozyme, is derived from the mitochondrial aI5γ intron of Saccharomyces cerevisiae and is ...
Choi, Woongsoon
core
Functional identification of catalytic metal ion binding sites within RNA. [PDF]
PLoS Biology, 2005 The viability of living systems depends inextricably on enzymes that catalyze phosphoryl transfer reactions. For many enzymes in this class, including several ribozymes, divalent metal ions serve as obligate cofactors.
James L Hougland +3 more
doaj +2 more sources
Multivalent RNA‐Cleaving Agents on a Cubic Octameric Silsesquioxane Core
Chemistry – A European Journal, EarlyView.RNA‐cleaving agents on a cubic octameric silsesquioxane core were synthesized and characterized. Several catalytic systems of these structures were screened using hexaribonucleotide models. The best systems were then further used to study their catalytic efficiency in cleaving a HER2 mRNA model sequence. The induced cleavage sites were also determined.
Hanni Haapsaari, Iris Tuomi, Pasi Virta
wiley +1 more source
Angewandte Chemie, Volume 138, Issue 27, 1 July 2026.This work presents the first enzymatic polymerization of defined metal‐chelator‐modified nucleotides that leverage polymerase fidelity for uniform (radio)metal loading with successful incorporation of five different metals: Ga, In, Tb, Lu, and Y.
Antonio A. W. L. Wong +2 more
wiley +2 more sources