Results 41 to 50 of about 27,058 (211)
Ligand‐dependent ribozymes [PDF]
WIREs RNA, 2016 The discovery of catalytic RNA (ribozymes) more than 30 years ago significantly widened the horizon of RNA‐based functions in natural systems. Similarly to the activity of protein enzymes that are often modulated by the presence of an interaction partner, some examples of naturally occurring ribozymes are influenced by ligands that can either act as ...
Felletti, Michele, Hartig, Jörg S.
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Angewandte Chemie, EarlyView.This work presents the first enzymatic polymerization of defined metal‐chelator‐modified nucleotides that leverage polymerase fidelity for uniform (radio)metal loading with successful incorporation of five different metals: Ga, In, Tb, Lu, and Y.
Antonio A. W. L. Wong +2 more
wiley +2 more sources
RNA interference approaches for treatment of HIV-1 infection. [PDF]
, 2015 HIV/AIDS is a chronic and debilitating disease that cannot be cured with current antiretroviral drugs. While combinatorial antiretroviral therapy (cART) can potently suppress HIV-1 replication and delay the onset of AIDS, viral mutagenesis often leads to
Bobbin, Maggie L +2 more
core +1 more source
RATEX: A Scalable RNA‐Based Platform for Logical and Multi‐Layered Cellular Programming
Angewandte Chemie, EarlyView.RATEX integrated ribosome‐mediated transcription control with synthetic RNA regulators, enabling simultaneous processing of RNA, metabolite, and small‐molecule inputs through complex logic operations within a single transcript. The platform achieves up to 1,492‐fold gene regulation, multi‐input hybrid logic gates, RNA‐programmed signaling cascades, and
Hyunseop Goh +3 more
wiley +2 more sources
Hairpin Ribozyme Genes Curtail Alcohol Drinking: from Rational Design to in vivo Effects in the Rat
Molecular Therapy: Nucleic Acids, 2016 Ribozyme genes were designed to reduce voluntary alcohol drinking in a rat model of alcohol dependence. Acetaldehyde generated from alcohol in the liver is metabolized by the mitochondrial aldehyde dehydrogenase (ALDH2) such that diminishing ALDH2 ...
Amalia Sapag +5 more
doaj +1 more source
Interplay of 'induced fit' and preorganization in the ligand induced folding of the aptamer domain of the guanine binding riboswitch [PDF]
, 2006 Riboswitches are highly structured elements in the 50-untranslated regions (50-UTRs) of messenger RNA that control gene expression by specifically binding to small metabolite molecules. They consist of an aptamer domain responsible for ligand binding and
Noeske, Jonas +5 more
core +1 more source
The Kiss Switch Brings Inactive R3C Ligase Ribozyme Back to Life
Biology, 2018 R3C ligase ribozyme catalyzes the nucleophilic attack by a 3′-hydroxyl on a 5′-α-phosphorus of triphosphates to form a 3′-5′-phosphodiester bond. In the present study, although the truncation of R3C ribozyme was accompanied by a large reduction in ...
Kana Tanizawa +4 more
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Journal of Investigative Dermatology Symposium Proceedings, 2002 Since their initial discovery, ribozymes have shown great promise not just as a tool in the manipulation of gene expression, but also as a novel therapeutic agent. This review discusses the promises and pitfalls of ribozyme technology, with a special emphasis on cancer-related applications, though relevance to skin disease will also be discussed.
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Angewandte Chemie, EarlyView.Current interest in artificial cell research underscores its potential to deepen our understanding of life's fundamental processes. This review highlights advances in bottom‐up coacervate‐based artificial cell engineering via combined integration of cellular hallmarks.
Arjan Hazegh Nikroo +3 more
wiley +2 more sources
Structure-based insights into self-cleavage by a four-way junctional twister-sister ribozyme
Nature Communications, 2017 Twister-sister is a self-cleaving ribozyme. Here, the authors report the 2.0 Å crystal structure of the four-way junctional twister-sister ribozyme in the pre-catalytic state and discuss mechanistic implications based on their mutagenesis experiments and
Luqian Zheng +7 more
doaj +1 more source