Results 61 to 70 of about 5,544 (121)

Enhancing CRISPR‐Cas12a base editing in plants with LbCas12a variants and introns

Journal of Integrative Plant Biology, Volume 68, Issue 9, Page 3243-3259, September 2026.
Intron optimization of LbCas12a‐RRV improves cytosine and adenine base editing efficiency in plants, supports multiplexed and double‐strand break‐free genome modification, and expands precise genome engineering tools for crop breeding and plant functional research. ABSTRACT Cytosine base editors (CBEs) and adenine base editors (ABEs) are powerful tools
Yanhao Cheng, Gen Li, Man Zhou, Rushil Mandlik, Doris Wang, Yiping Qi   +5 more
wiley   +1 more source

RNA-peptide interactions tune the ribozyme activity within coacervate microdroplet dispersions

Nature Communications
Membrane-free complex coacervate microdroplets are compelling models for primitive compartmentalisation with the ability to form from biological molecules.
Basusree Ghosh, Patrick M. McCall, Kristian Kyle Le Vay, Archishman Ghosh, Lars Hubatsch, David T. Gonzales, Jan Brugués, Hannes Mutschler, T-Y. Dora Tang   +8 more
doaj   +1 more source

Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme

Nature Communications, 2020
RNA sequence-structure-function relationships can be studied via assays of variant activity. The authors developed a method to systematically determine ribozyme variant kinetics, using it to map double-mutant interactions and show that cleavage rate ...
Johan O. L. Andreasson, Andrew Savinov, Steven M. Block, William J. Greenleaf   +3 more
doaj   +1 more source

The effects of anti-Fas ribozyme on T lymphocyte apoptosis in mice model with chronic obstructive pulmonary disease [PDF]

Iranian Journal of Basic Medical Sciences, 2017
Objective(s): In this study, we aimed to investigate the effects of anti-Fas ribozyme on the apoptosis of T lymphocytes (T cells) in mice model with chronic obstructive pulmonary disease (COPD).
Song-Ming Zhuo, Si-Cong Li, Yong-Qun Lin, Hai-Bin Yu, Na Li   +4 more
doaj   +1 more source

Dynamic Control of Nucleic Acids Self‐Assembly and Expression Using Photoswitches

Chemistry – A European Journal, Volume 32, Issue 31, 18 August 2026.
We review here the recent progress made in the design of molecular photoswitches, and highlight their implementation for the dynamic control over nucleic acids self‐assembly and expression. ABSTRACT Synthetic nucleic acids have become readily available and now constitute versatile building blocks in materials science—where they can be used to engineer ...
Noemí Nogal, Hugo Laigle, Maxime Mansy, Sébastien Ulrich, Mathieu Surin   +4 more
wiley   +1 more source

Inhibition of Cpeb3 ribozyme elevates CPEB3 protein expression and polyadenylation of its target mRNAs and enhances object location memory

eLife
A self-cleaving ribozyme that maps to an intron of the cytoplasmic polyadenylation element-binding protein 3 (Cpeb3) gene is thought to play a role in human episodic memory, but the underlying mechanisms mediating this effect are not known. We tested the
Claire C Chen, Joseph Han, Carlene A Chinn, Jacob S Rounds, Xiang Li, Mehran Nikan, Marie Myszka, Liqi Tong, Luiz FM Passalacqua, Timothy Bredy, Marcelo A Wood, Andrej Luptak   +11 more
doaj   +1 more source

Evidence for the involvement of G_i2 in activation of extracellular signal-regulated kinases in hepatocytes

BMC Cell Biology, 2001
Background Activation of the extracellular signal-regulated kinases ERK1 and ERK2 in hepatocytes by prostaglandin (PG)F2α was recently found to be inhibited by pertussis toxin (PTX) suggesting a role for Gi proteins. Results Targeting the Gi2α expression
Sioud Mouldy, Christoffersen Thoralf, Melien Øyvind   +2 more
doaj   +1 more source

Integrative in silico and in vitro screening of small molecules targeting RNA

Current Protocols, Volume 6, Issue 8, August 2026.
Abstract Targeting structured RNA elements with small molecules has emerged as a promising yet technically challenging strategy for antiviral drug discovery. Here, we present a comprehensive and experimentally validated workflow for the integrative in silico and in vitro screening of RNA‐binding small molecules.
Sabrina Toews, Megan Ken, Harald Schwalbe   +2 more
wiley   +1 more source

Tumour‐Derived Extracellular Vesicles Containing dsRNA Induce Degradation of Ribosomal Protein mRNA in Platelets

Journal of Extracellular Vesicles, Volume 15, Issue 8, August 2026.
ABSTRACT Extracellular vesicles (EVs) mediate intercellular communication within the tumour microenvironment by carrying cargoes from paracrine parent cells. EVs have attracted great research interest for their ability to carry nucleic acids into recipient cells and modulate cellular functions.
Gaoge Sun, Yuhuan Tao, Zihan Liu, Kaixiang Zhang, Shuai Zuo, Shanwen Chen, Shufang Ning, Tianyi Zhang, Pengyuan Wang, Ying Zhang, Zhi John Lu, Hang Hubert Yin   +11 more
wiley   +1 more source

Efficient inhibition of hepatitis B virus replication by hepatitis delta virus ribozymes delivered by targeting retrovirus

Virology Journal, 2010
Background Hepatitis delta virus (HDV) ribozyme is an attractive molecular tool that can specifically recognize and catalyze the self-cleavage of the viral RNA phosphodiester backbone.
Chen Long-Hua, Qi Peng, Lu Yan-Qin, Wang Chuan-Xi, Han Jin-Xiang   +4 more
doaj   +1 more source