Results 61 to 70 of about 18,960 (170)

Evidence for the involvement of G_i2 in activation of extracellular signal-regulated kinases in hepatocytes

BMC Cell Biology, 2001
Background Activation of the extracellular signal-regulated kinases ERK1 and ERK2 in hepatocytes by prostaglandin (PG)F2α was recently found to be inhibited by pertussis toxin (PTX) suggesting a role for Gi proteins. Results Targeting the Gi2α expression
Sioud Mouldy, Christoffersen Thoralf, Melien Øyvind   +2 more
doaj   +1 more source

Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme

Nature Communications, 2020
RNA sequence-structure-function relationships can be studied via assays of variant activity. The authors developed a method to systematically determine ribozyme variant kinetics, using it to map double-mutant interactions and show that cleavage rate ...
Johan O. L. Andreasson, Andrew Savinov, Steven M. Block, William J. Greenleaf   +3 more
doaj   +1 more source

The effects of anti-Fas ribozyme on T lymphocyte apoptosis in mice model with chronic obstructive pulmonary disease [PDF]

Iranian Journal of Basic Medical Sciences, 2017
Objective(s): In this study, we aimed to investigate the effects of anti-Fas ribozyme on the apoptosis of T lymphocytes (T cells) in mice model with chronic obstructive pulmonary disease (COPD).
Song-Ming Zhuo, Si-Cong Li, Yong-Qun Lin, Hai-Bin Yu, Na Li   +4 more
doaj   +1 more source

Nucleobase Participation in Ribozyme Catalysis

, 2016
We constructed a modified form of the VS ribozyme containing an imidazole ring in place of adenine at position 756. The novel ribozyme is active in both cleavage and ligation reactions. The reaction is efficient, although relatively slow. The results are
Zheng-yun Zhao (587046), Lisa Araki (2385367), Takushi Kurihara (2447893), David M. J. Lilley (245907), Maho Yamaguchi (1842334), Aileen McLeod (2686453), Shinya Harusawa (1777027)   +6 more
core   +1 more source

Inhibition of Cpeb3 ribozyme elevates CPEB3 protein expression and polyadenylation of its target mRNAs and enhances object location memory

eLife
A self-cleaving ribozyme that maps to an intron of the cytoplasmic polyadenylation element-binding protein 3 (Cpeb3) gene is thought to play a role in human episodic memory, but the underlying mechanisms mediating this effect are not known. We tested the
Claire C Chen, Joseph Han, Carlene A Chinn, Jacob S Rounds, Xiang Li, Mehran Nikan, Marie Myszka, Liqi Tong, Luiz FM Passalacqua, Timothy Bredy, Marcelo A Wood, Andrej Luptak   +11 more
doaj   +1 more source

Isothiazologuanosine (tzG) as Environmentally Sensitive Fluorescent Reporter in RNA

Angewandte Chemie, Volume 138, Issue 43, 19 October 2026.
The emissive isothiazologuanosine analog tzG is synthesized for the first time as RNA phosphoramidite building block for solid‐phase synthesis. Due to its isomorphic and isofunctional characteristics, tzG forms Watson–Crick base pairs and can be used as fluorescent probe to monitor (deoxy)ribozyme activity.
Julia Dietzsch, Yitzhak Tor, Claudia Höbartner   +2 more
wiley   +1 more source

Efficient inhibition of hepatitis B virus replication by hepatitis delta virus ribozymes delivered by targeting retrovirus

Virology Journal, 2010
Background Hepatitis delta virus (HDV) ribozyme is an attractive molecular tool that can specifically recognize and catalyze the self-cleavage of the viral RNA phosphodiester backbone.
Chen Long-Hua, Qi Peng, Lu Yan-Qin, Wang Chuan-Xi, Han Jin-Xiang   +4 more
doaj   +1 more source

In Vitro Selection and Characterization of a Novel Zn(II)-Dependent Phosphorothiolate Thiolesterase Ribozyme

, 2016
Here we present the in vitro selection of a novel ribozyme specific for Zn2+-dependent catalysis on hydrolysis of a phosphorothiolate thiolester bond. The ribozyme, called the TW17 ribozyme, was evolved and selected from an artificial RNA pool covalently
Kun-Liang Lin (2129005), Eng-Chi Wang (2011303), Yi-Ming Liou (2129008), Tzu-Pin Wang (415062), Yen-Hsu Chen (314564), Yu-Chih Su (2011300), Yi Chen (38576), Long-Chih Hwang (1425886), Yun-Ming Wang (1737091)   +8 more
core   +1 more source

An Electrophilic Uridine Building Block for Post‐Synthetic RNA Modification as Exemplified for Spin Labeling

Chemistry – A European Journal, Volume 32, Issue 38, 13 October 2026.
An electrophilic 5‐isothiocyanatomethyl uridine building block enables modular post‐synthetic RNA labeling with primary amine‐containing probes. Positioning the functionality in the major groove minimizes structural perturbation and allows facile conjugation of sensitive tags such as TEMPO spin labels for paramagnetic relaxation enhancement studies ...
Lisa Ruetz, Sonja Leiner, Alessandro Marotto, Raphael Plangger, Christoph Kreutz   +4 more
wiley   +1 more source

A selected ribozyme catalyzing diverse dipeptide synthesis

, 2009
The sequence of events by which protein, RNA, and DNA emerged during early biological evolution is one of the most profound questions regarding the origin of life.
Sun, Lele, Cui, Zhiyong, Zhang, Biliang, Gottlieb, Robert L.   +3 more
core   +2 more sources