Results 61 to 70 of about 27,058 (211)
Inducible knockdown of Plasmodium gene expression using the glmS ribozyme.
PLoS ONE, 2013 Conventional reverse genetic approaches for study of Plasmodium malaria parasite gene function are limited, or not applicable. Hence, new inducible systems are needed. Here we describe a method to control P.
Parichat Prommana +7 more
doaj +1 more source
Design and Preparation of a Multimeric Self-Cleaving Hammerhead Ribozyme
BioTechniques, 1997 The activity of a ribozyme can be impaired by additional sequences at the 5′ and 3′ termini of the catalytic sequence. To approach this problem, a system was designed that minimizes sequences upstream and downstream from active regions of a hammerhead ...
Juan Ruiz +3 more
doaj +1 more source
Ribozymes for RNA‐Catalyzed RNA Methylation and Labeling
Angewandte Chemie International Edition, EarlyView.Ribozymes are powerful tools for site‐specific RNA modification. Their activities range from installing tags and fluorophores to generating natural RNA methylations, making them valuable tools to uncover the many functions that RNA plays in nature.
Carolin P. M. Scheitl +1 more
wiley +1 more source
Method of identifying inhibitors of glutathione S-transferase (GST) gene expression [PDF]
, 1999 Complementary DNA and genomic clones for three variants of GST-.pi. are disclosed. It is demonstrated that certain of these variants are overexpressed in gliomas, thereby indicating an involvement with that form of cancer.
Akande, Olanike +6 more
core +1 more source
Large phenotype jumps in biomolecular evolution
, 2003 By defining the phenotype of a biopolymer by its active three-dimensional shape, and its genotype by its primary sequence, we propose a model that predicts and characterizes the statistical distribution of a population of biopolymers with a specific ...
A.S. Perelson +20 more
core +3 more sources
Biochemistry and Molecular Biology Education, EarlyView.ABSTRACT Traditional biochemistry instruction often emphasizes mechanistic detail, that is, how molecules and pathways function, without equally addressing why they have their present forms. This fact‐centered approach can leave students overwhelmed and disconnected from the broader scientific narrative.
Alberto Vázquez‐Salazar
wiley +1 more source
eLifeA self-cleaving ribozyme that maps to an intron of the cytoplasmic polyadenylation element-binding protein 3 (Cpeb3) gene is thought to play a role in human episodic memory, but the underlying mechanisms mediating this effect are not known. We tested the
Claire C Chen +11 more
doaj +1 more source
CPEB3 is associated with human episodic memory
Frontiers in Behavioral Neuroscience, 2009 Cytoplasmic polyadenylation element-binding (CPEB) proteins are crucial for synaptic plasticity and memory in model organisms. A highly conserved, mammalian-specific short intronic sequence within CPEB3 has been identified as a ribozyme with self ...
Christian Vogler +14 more
doaj +1 more source
Correcting pervasive errors in RNA crystallography through enumerative structure prediction
, 2012 Three-dimensional RNA models fitted into crystallographic density maps exhibit pervasive conformational ambiguities, geometric errors and steric clashes. To address these problems, we present enumerative real-space refinement assisted by electron density
AT Brunger +27 more
core +1 more source
Dynamic Control of Nucleic Acids Self‐Assembly and Expression Using Photoswitches
Chemistry – A European Journal, EarlyView.We review here the recent progress made in the design of molecular photoswitches, and highlight their implementation for the dynamic control over nucleic acids self‐assembly and expression. ABSTRACT Synthetic nucleic acids have become readily available and now constitute versatile building blocks in materials science—where they can be used to engineer ...
Noemí Nogal +4 more
wiley +1 more source