Results 31 to 40 of about 18,960 (170)

Ribozyme activity modulates the physical properties of RNA–peptide coacervates

eLife, 2023
Condensed coacervate phases are now understood to be important features of modern cell biology, as well as valuable protocellular models in origin-of-life studies and synthetic biology.
Kristian Kyle Le Vay, Elia Salibi, Basusree Ghosh, TY Dora Tang, Hannes Mutschler   +4 more
doaj   +1 more source

Molecular characterization of a new member of the lariat capping twin-ribozyme introns [PDF]

, 2014
Background: Twin-ribozyme introns represent a complex class of mobile group I introns that harbour a lariat capping (LC) ribozyme and a homing endonuclease gene embedded in a conventional self-splicing group I ribozyme (GIR2).
Yunjia Tang, Tang, Y. (Yunjia), Nielsen, H., Masquida, B. (Benoit), Tang, Yunjia, Benoît Masquida, Johansen, S.D., Paul P Gardner, Nielsen, H. (Henrik), Henrik Nielsen, Gardner, P.P., Masquida, B., Masquida, Benoît, Johansen, Steinar D, Tang, Y., Johansen, Steinard D.., Yunjia Tang, Gardner, Paul P, Steinar D Johansen, Gardner, Paul P.., Nielsen, Henrik, Johansen, Steinar, Gardner, Paul   +22 more
core   +1 more source

Identification of selective hepatitis delta virus ribozyme inhibitors by high-throughput screening of small molecule libraries

JHEP Reports, 2023
Background & Aims: Chronic hepatitis delta is the most severe form of chronic viral hepatitis and is associated with faster progression towards cirrhosis, liver decompensation, and hepatocellular carcinoma.
Eirini D. Tseligka, Stéphanie Conzelmann, Yves Cambet, Tifany Schaer, Francesco Negro, Sophie Clément   +5 more
doaj   +1 more source

The Hammerhead Ribozyme: A Long History for a Short RNA

Molecules, 2017
Small nucleolytic ribozymes are a family of naturally occurring RNA motifs that catalyse a self-transesterification reaction in a highly sequence-specific manner.
Marcos de la Peña, Inmaculada García-Robles, Amelia Cervera   +2 more
doaj   +1 more source

Ribozyme-Mediated Cleavage of a Substrate Analogue Containing an Internucleotide-Bridging 5‘-Phosphorothioate:  Evidence for the Single-Metal Model^†

, 2016
An oligonucleotide substrate containing a 5‘-bridging phosphorothioate linkage adjacent to a ribonucleotide has been used to investigate the cleavage mechanism of the hammerhead ribozyme and to probe the catalytic role of the metal cofactor(s ...
Robert G. Kuimelis (2948322), Larry W. McLaughlin (10778)   +1 more
core   +1 more source

Hairpin Ribozyme Structure and Dynamics

, 1997
A wide range of experimental methods have been used to explore the structure and function of the hairpin ribozyme. In vitro selection methods has provided comprehensive information on RNA secondary structure and the identity of essential bases within the
Banerjee, Aloke Raj, J. E. Heckman, Esteban, José A., Berzal-Herranz, Alfredo, Heckman, Joyce, N. Walter, Walter, Nils, A. R. Banerjee, Burke, John M., J. A. Esteban, J. Bond, B. Sargueil, A. Berzal-Herranz, Bond, J., Sargueil, B., S. Butcher, Butcher, S., J. M. Burke   +17 more
core   +1 more source

In vitro Evolution and Engineering for Improved Ribozyme Polymerase Production [PDF]

, 2011
According to the "RNA World Hypothesis" the first self replicating molecule formed from the prebiotic chemicals is the ribozyme. A model compartmentalized self replicating system for ribozyme polymerases is designed and used to improve activity,
Muhammad Waheed Akktar, Ayaz Ali Khan, Israr Khan   +2 more
core  

Aminoacyl-tRNA Synthesis by a Resin-Immobilized Ribozyme

, 2016
We report herein a new method for the aminoacylation of tRNA, using a resin-immobilized ribozyme and the cyanomethyl ester (CME) of an amino acid substrate. The oxidized form of the ribozyme was immobilized on a hydrazine resin via covalent linkage.
Hiroshi Murakami (227877), Hiroaki Suga (295809), Neil J. Bonzagni (2989293)   +2 more
core   +1 more source

Probing General Base Catalysis in the Hammerhead Ribozyme

, 2016
Recent structural and computational studies have shed new light on the catalytic mechanism and active site structure of the RNA cleaving hammerhead ribozyme. Consequently, specific ribozyme functional groups have been hypothesized to be directly involved
Jason M. Thomas (2379268), David M. Perrin (1795915)   +1 more
core   +2 more sources

Characterization of D135 group II intron ribozyme dimerization [PDF]

, 2013
textGroup II introns are highly structured RNAs that carry out self-splicing reactions. The multiple turnover version of one of these introns, termed the D135 ribozyme, is derived from the mitochondrial aI5γ intron of Saccharomyces cerevisiae and is ...
Choi, Woongsoon
core