A Faster Triphosphorylation Ribozyme. [PDF]
PLoS ONE, 2015 In support of the RNA world hypothesis, previous studies identified trimetaphosphate (Tmp) as a plausible energy source for RNA world organisms. In one of these studies, catalytic RNAs (ribozymes) that catalyze the triphosphorylation of RNA 5'-hydroxyl ...
Gregory F Dolan +2 more
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Arginine cofactors on the polymerase ribozyme. [PDF]
PLoS ONE, 2011 The RNA world hypothesis states that the early evolution of life went through a stage in which RNA served both as genome and as catalyst. The central catalyst in an RNA world organism would have been a ribozyme that catalyzed RNA polymerization to ...
Chengguo Yao +4 more
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Structure-based insights into self-cleavage by a four-way junctional twister-sister ribozyme
Nature Communications, 2017 Twister-sister is a self-cleaving ribozyme. Here, the authors report the 2.0 Å crystal structure of the four-way junctional twister-sister ribozyme in the pre-catalytic state and discuss mechanistic implications based on their mutagenesis experiments and
Luqian Zheng +7 more
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RNA-Guided Engineering of the Chloroplast Genome Enabled by Plastid-Expressed Guide RNAs. [PDF]
Plant Biotechnol JABSTRACT Our goal is to develop RNA‐guided engineering of the chloroplast genome using the CRISPR/Cas9 system. We designed chloroplast minigenes to obtain properly sized single guide RNAs (sgRNAs) in tobacco chloroplasts. The sgRNA 5′ end is defined by transcription from an rRNA operon promoter, and its 3′ end by processing a downstream tRNA (trnG) or ...
Mirzaee M +3 more
europepmc +2 more sources
Isothiazologuanosine (<sup>tz</sup>G) as Environmentally Sensitive Fluorescent Reporter in RNA. [PDF]
Angew Chem Int Ed EnglThe emissive isothiazologuanosine analog tzG is synthesized for the first time as RNA phosphoramidite building block for solid‐phase synthesis. Due to its isomorphic and isofunctional characteristics, tzG forms Watson–Crick base pairs and can be used as fluorescent probe to monitor (deoxy)ribozyme activity.
Dietzsch J, Tor Y, Höbartner C.
europepmc +2 more sources
Establishment of a genome editing system and expression of heterologous proteins in the nonconventional yeast <i>Pichia kudriavzevii</i>. [PDF]
mLifemLife, EarlyView.
Ma J, Cui Y, Yin B, Jin C.
europepmc +2 more sources
Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression [PDF]
, 2014 Organisms have different circuitries that allow converting signal molecule levels to changes in gene expression. An important challenge in synthetic biology involves the de novo design of RNA modules enabling dynamic signal processing in live cells. This
Satya Prakash +21 more
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, 2021 The lariat capping ribozyme (LCrz) constitutes an independent class of ribozymes. It is the defining feature of twin-ribozyme group I introns found in eukaryotic microorganisms.
Steinar Daae Johansen +8 more
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Fluorescence Polarization for Monitoring Ribozyme Reactions in Real Time
BioTechniques, 2000 Fluorescence polarization has been used recently to monitor diverse macromolecular interactions. In this report, the application of fluorescence polarization has been extended to monitor ribozyme reactions in real time.
K.K. Singh +4 more
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Inspecting the Ribozyme Region of Hepatitis Delta Virus Genotype 1: Conservation and Variability
Viruses, 2022 The hepatitis delta virus (HDV) genome has an autocatalytic region called the ribozyme, which is essential for viral replication. The aim of this study was to use next-generation sequencing (NGS) to analyze the ribozyme quasispecies (QS) in order to ...
Beatriz Pacin-Ruiz +17 more
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