Results 11 to 20 of about 27,058 (211)

RNase P Ribozymes Inhibit the Replication of Human Cytomegalovirus by Targeting Essential Viral Capsid Proteins [PDF]

Viruses, 2015
An engineered RNase P-based ribozyme variant, which was generated using the in vitro selection procedure, was used to target the overlapping mRNA region of two proteins essential for human cytomegalovirus (HCMV) replication: capsid assembly protein (AP ...
Zhu Yang, Michael Reeves, Jun Ye, Phong Trang, Li Zhu, Jingxue Sheng, Yu Wang, Ke Zen, Jianguo Wu, Fenyong Liu   +9 more
doaj   +5 more sources

Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme [PDF]

Nature Communications, 2020
RNA sequence-structure-function relationships can be studied via assays of variant activity. The authors developed a method to systematically determine ribozyme variant kinetics, using it to map double-mutant interactions and show that cleavage rate ...
Johan O. L. Andreasson, Andrew Savinov, Steven M. Block, William J. Greenleaf   +3 more
doaj   +2 more sources

RNA-peptide interactions tune the ribozyme activity within coacervate microdroplet dispersions [PDF]

Nature Communications
Membrane-free complex coacervate microdroplets are compelling models for primitive compartmentalisation with the ability to form from biological molecules.
Basusree Ghosh, Patrick M. McCall, Kristian Kyle Le Vay, Archishman Ghosh, Lars Hubatsch, David T. Gonzales, Jan Brugués, Hannes Mutschler, T-Y. Dora Tang   +8 more
doaj   +2 more sources

A Faster Triphosphorylation Ribozyme. [PDF]

PLoS ONE, 2015
In support of the RNA world hypothesis, previous studies identified trimetaphosphate (Tmp) as a plausible energy source for RNA world organisms. In one of these studies, catalytic RNAs (ribozymes) that catalyze the triphosphorylation of RNA 5'-hydroxyl ...
Gregory F Dolan, Arvin Akoopie, Ulrich F Müller   +2 more
doaj   +7 more sources

Ribozyme-Mediated Knockdown of lncRNA Gene Expression in Drosophila [PDF]

Bio-Protocol
Long noncoding RNAs (lncRNAs) are increasingly understood to play important roles in cell biology, development, and disease, though the vast majority of annotated lncRNAs have yet to be functionally characterized.
Kevin Nyberg, Richard Carthew
doaj   +2 more sources

Structural Expansion of Catalytic RNA Nanostructures through Oligomerization of a Cyclic Trimer of Engineered Ribozymes

Molecules, 2023
The multimolecular assembly of three-dimensionally structured proteins forms their quaternary structures, some of which have high geometric symmetry. The size and complexity of protein quaternary structures often increase in a hierarchical manner, with ...
Mst. Ayesha Siddika, Hiroki Oi, Kumi Hidaka, Hiroshi Sugiyama, Masayuki Endo, Shigeyoshi Matsumura, Yoshiya Ikawa   +6 more
doaj   +1 more source

RNase P Ribozyme Effectively Inhibits Human CC-Chemokine Receptor 5 Expression and Human Immunodeficiency Virus 1 Infection

Zoonotic Diseases, 2023
Developing novel antiviral agents and approaches is essential for the treatment against human and zoonotic viruses. We had previously produced RNase P-based ribozyme variants capable of efficiently cleaving mRNA in vitro.
Bin Yan, Yujun Liu, Yuan-Chuan Chen, Isadora Zhang, Fenyong Liu   +4 more
doaj   +1 more source

A convolutional neural network for the prediction and forward design of ribozyme-based gene-control elements

eLife, 2021
Ribozyme switches are a class of RNA-encoded genetic switch that support conditional regulation of gene expression across diverse organisms. An improved elucidation of the relationships between sequence, structure, and activity can improve our capacity ...
Calvin M Schmidt, Christina D Smolke
doaj   +1 more source

Effective inhibition of human immunodeficiency virus 1 replication by engineered RNase P ribozyme. [PDF]

PLoS ONE, 2012
Using an in vitro selection procedure, we have previously isolated RNase P ribozyme variants that efficiently cleave an mRNA sequence in vitro. In this study, a variant was used to target the HIV RNA sequence in the tat region.
Wenbo Zeng, Yuan-Chuan Chen, Yong Bai, Phong Trang, Gia-Phong Vu, Sangwei Lu, Jianguo Wu, Fenyong Liu   +7 more
doaj   +1 more source

Catalytic RNA Oligomers Formed by Co-Oligomerization of a Pair of Bimolecular RNase P Ribozymes

Molecules, 2022
Naturally occurring ribozymes with a modular architecture are promising platforms for construction of RNA nanostructures because modular redesign enables their oligomerization.
Mst. Ayesha Siddika, Takahiro Yamada, Risako Aoyama, Kumi Hidaka, Hiroshi Sugiyama, Masayuki Endo, Shigeyoshi Matsumura, Yoshiya Ikawa   +7 more
doaj   +1 more source