Results 11 to 20 of about 5,544 (121)
Inspecting the Ribozyme Region of Hepatitis Delta Virus Genotype 1: Conservation and Variability
Viruses, 2022 The hepatitis delta virus (HDV) genome has an autocatalytic region called the ribozyme, which is essential for viral replication. The aim of this study was to use next-generation sequencing (NGS) to analyze the ribozyme quasispecies (QS) in order to ...
Beatriz Pacin-Ruiz +17 more
doaj +1 more source
Fluorescence Polarization for Monitoring Ribozyme Reactions in Real Time
BioTechniques, 2000 Fluorescence polarization has been used recently to monitor diverse macromolecular interactions. In this report, the application of fluorescence polarization has been extended to monitor ribozyme reactions in real time.
K.K. Singh +4 more
doaj +1 more source
Communications Biology, 2023 Investigating gene function relies on the efficient manipulation of endogenous gene expression. Currently, a limited number of tools are available to robustly manipulate endogenous gene expression between “on” and “off” states. In this study, we insert a
Jie Fang +5 more
doaj +1 more source
Biology, 2014 The R3C ligase ribozyme is an artificial ligase ribozyme produced by modification of the ribozyme that lacks cytidine. Here, we attempted to modify the original R3C ribozyme (73 nucleotides) by reducing the number of nucleotides while maintaining the ...
Eri Kurihara +3 more
doaj +1 more source
A ribozyme-based regulatory system.
, 2022 A ribozyme is inserted into the 3’ untranslated region between the translation termination codon of the gene coding sequence and the poly-A tail. The ribozyme contains an aptamer to theophylline (purple) integrated into one of the hammerhead ribozyme’s ...
Christina D. Smolke (264988) +2 more
core +1 more source
Angewandte Chemie, EarlyView.Dehydroamino acids (DHAA) are atypical electrophilic sites that lead to crosslinked proteins in Alzheimer's disease (AD) brains. No catalyst for their formation has been known in humans. We report that metal oxides catalyze β‐elimination of phosphoserine and phosphothreonine in model peptides at pH 7.5°C and 37°C, yielding DHAAs.
Samuel W. Markovich +3 more
wiley +2 more sources
Inhibition of human cytomegalovirus immediate early gene expression and growth by a novel RNase P ribozyme variant. [PDF]
PLoS ONE, 2017 We have previously engineered new RNase P-based ribozyme variants with improved in vitro catalytic activity. In this study, we employed a novel engineered variant to target a shared mRNA region of human cytomegalovirus (HCMV) immediate early proteins 1 ...
Xu Sun +13 more
doaj +1 more source
Viruses, 2015 An engineered RNase P-based ribozyme variant, which was generated using the in vitro selection procedure, was used to target the overlapping mRNA region of two proteins essential for human cytomegalovirus (HCMV) replication: capsid assembly protein (AP ...
Zhu Yang +9 more
doaj +1 more source
A RNase P Ribozyme Inhibits Gene Expression and Replication of Hepatitis B Virus in Cultured Cells
Microorganisms, 2023 Hepatitis B virus (HBV), an international public health concern, is a leading viral cause of liver disease, such as hepatocellular carcinoma. Sequence-specific ribozymes derived from ribonuclease P (RNase P) catalytic RNA are being explored for gene ...
Bin Yan +3 more
doaj +1 more source
Hairpin Ribozyme Genes Curtail Alcohol Drinking: from Rational Design to in vivo Effects in the Rat
Molecular Therapy: Nucleic Acids, 2016 Ribozyme genes were designed to reduce voluntary alcohol drinking in a rat model of alcohol dependence. Acetaldehyde generated from alcohol in the liver is metabolized by the mitochondrial aldehyde dehydrogenase (ALDH2) such that diminishing ALDH2 ...
Amalia Sapag +5 more
doaj +1 more source