Results 21 to 30 of about 16,956 (222)
Ligand‐dependent ribozymes [PDF]
WIREs RNA, 2016 The discovery of catalytic RNA (ribozymes) more than 30 years ago significantly widened the horizon of RNA‐based functions in natural systems. Similarly to the activity of protein enzymes that are often modulated by the presence of an interaction partner, some examples of naturally occurring ribozymes are influenced by ligands that can either act as ...
Felletti, Michele, Hartig, Jörg S.
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A ribozyme-based regulatory system.
, 2022 A ribozyme is inserted into the 3’ untranslated region between the translation termination codon of the gene coding sequence and the poly-A tail. The ribozyme contains an aptamer to theophylline (purple) integrated into one of the hammerhead ribozyme’s ...
Christina D. Smolke (264988) +2 more
core +1 more source
A RNase P Ribozyme Inhibits Gene Expression and Replication of Hepatitis B Virus in Cultured Cells
Microorganisms, 2023 Hepatitis B virus (HBV), an international public health concern, is a leading viral cause of liver disease, such as hepatocellular carcinoma. Sequence-specific ribozymes derived from ribonuclease P (RNase P) catalytic RNA are being explored for gene ...
Bin Yan +3 more
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Inhibition of human cytomegalovirus immediate early gene expression and growth by a novel RNase P ribozyme variant. [PDF]
PLoS ONE, 2017 We have previously engineered new RNase P-based ribozyme variants with improved in vitro catalytic activity. In this study, we employed a novel engineered variant to target a shared mRNA region of human cytomegalovirus (HCMV) immediate early proteins 1 ...
Xu Sun +13 more
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Viruses, 2015 An engineered RNase P-based ribozyme variant, which was generated using the in vitro selection procedure, was used to target the overlapping mRNA region of two proteins essential for human cytomegalovirus (HCMV) replication: capsid assembly protein (AP ...
Zhu Yang +9 more
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Hairpin Ribozyme Genes Curtail Alcohol Drinking: from Rational Design to in vivo Effects in the Rat
Molecular Therapy: Nucleic Acids, 2016 Ribozyme genes were designed to reduce voluntary alcohol drinking in a rat model of alcohol dependence. Acetaldehyde generated from alcohol in the liver is metabolized by the mitochondrial aldehyde dehydrogenase (ALDH2) such that diminishing ALDH2 ...
Amalia Sapag +5 more
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Journal of Investigative Dermatology Symposium Proceedings, 2002 Since their initial discovery, ribozymes have shown great promise not just as a tool in the manipulation of gene expression, but also as a novel therapeutic agent. This review discusses the promises and pitfalls of ribozyme technology, with a special emphasis on cancer-related applications, though relevance to skin disease will also be discussed.
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Structure-based insights into self-cleavage by a four-way junctional twister-sister ribozyme
Nature Communications, 2017 Twister-sister is a self-cleaving ribozyme. Here, the authors report the 2.0 Å crystal structure of the four-way junctional twister-sister ribozyme in the pre-catalytic state and discuss mechanistic implications based on their mutagenesis experiments and
Luqian Zheng +7 more
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The Kiss Switch Brings Inactive R3C Ligase Ribozyme Back to Life
Biology, 2018 R3C ligase ribozyme catalyzes the nucleophilic attack by a 3′-hydroxyl on a 5′-α-phosphorus of triphosphates to form a 3′-5′-phosphodiester bond. In the present study, although the truncation of R3C ribozyme was accompanied by a large reduction in ...
Kana Tanizawa +4 more
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, 2021 The lariat capping ribozyme (LCrz) constitutes an independent class of ribozymes. It is the defining feature of twin-ribozyme group I introns found in eukaryotic microorganisms.
Steinar Daae Johansen +8 more
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