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Hammerhead ribozymes directed against mRNA of an essential gene inhibit Escherichia coli growth and enhance tetracycline efficacy [PDF]
Frontiers in MicrobiologyAiming to find novel ways to inhibit bacterial growth, we tested hammerhead ribozymes targeting the mRNAacpP transcript, which encodes the essential acyl carrier protein in Escherichia coli.
Joanna Miszkiewicz-Golec +6 more
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Potential Applications of RNase P Ribozyme Against Hepatitis B Virus [PDF]
MoleculesNucleic acid-based gene-interfering molecules, such as antisense oligonucleotides, ribozymes, and small interfering RNA (siRNA), represent exciting gene-targeting agents for therapeutic applications.
Thomas Sorrell, Yujun Liu, Fenyong Liu
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Minimal twister sister-like self-cleaving ribozymes in the human genome revealed by deep mutational scanning [PDF]
eLifeDespite their importance in a wide range of living organisms, self-cleaving ribozymes in the human genome are few and poorly studied. Here, we performed deep mutational scanning and covariance analysis of two previously proposed self-cleaving ribozymes ...
Zhe Zhang +5 more
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Programming anti-ribozymes to sense trigger RNAs for modulating gene expression in mammalian cells [PDF]
Synthetic and Systems BiotechnologySynthetic RNA-based switches provide distinctive merits in modulating gene expression. Simple and flexible RNA-based switches are crucial for advancing the field of gene regulation, paving the way for innovative tools that can sense and manipulate ...
Wenhui Zhang +5 more
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Twister ribozymes as highly versatile expression platforms for artificial riboswitches
Nature Communications, 2016 Twister ribozymes are small endonucleolytic RNA motifs. Here the authors develop twister ribozymes into RNA logic gates and cross-species synthetic genetic regulators.
Michele Felletti, Jörg S Hartig
exaly +2 more sources
Molecular Therapy: Nucleic Acids, 2022 The naturally occurring structure and biological functions of RNA are correlated, which includes hammerhead ribozymes. We proposed new variants of hammerhead ribozymes targeting conserved structural motifs of segment 5 of influenza A virus (IAV) (+)RNA ...
Tomasz Czapik +3 more
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A scalable system for the fast production of RNA with homogeneous terminal ends
RNA Biology, 2022 In vitro transcription (IVT) using T7 RNA polymerase has become the most common method to synthesize RNAs for use in basic research and pharmaceutical applications.
Yuchen Chen, Yan Cheng, Jinzhong Lin
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Single Mutation in Hammerhead Ribozyme Favors Cleavage Activity with Manganese over Magnesium
Non-Coding RNA, 2020 Hammerhead ribozymes are one of the most studied classes of ribozymes so far, from both the structural and biochemical point of views. The activity of most hammerhead ribozymes is cation-dependent. Mg2+ is one of the most abundant divalent cations in the
Mohammad Reza Naghdi +4 more
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eLife, 2023 Self-cleaving ribozymes are RNA molecules that catalyze the cleavage of their own phosphodiester backbones. These ribozymes are found in all domains of life and are also a tool for biotechnical and synthetic biology applications.
Jessica M Roberts +4 more
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Ligand‐dependent ribozymes [PDF]
WIREs RNA, 2016 The discovery of catalytic RNA (ribozymes) more than 30 years ago significantly widened the horizon of RNA‐based functions in natural systems. Similarly to the activity of protein enzymes that are often modulated by the presence of an interaction partner, some examples of naturally occurring ribozymes are influenced by ligands that can either act as ...
Felletti, Michele, Hartig, Jörg S.
openaire +3 more sources