Minimal twister sister-like self-cleaving ribozymes in the human genome revealed by deep mutational scanning [PDF]
eLifeDespite their importance in a wide range of living organisms, self-cleaving ribozymes in the human genome are few and poorly studied. Here, we performed deep mutational scanning and covariance analysis of two previously proposed self-cleaving ribozymes ...
Zhe Zhang +5 more
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Programming anti-ribozymes to sense trigger RNAs for modulating gene expression in mammalian cells [PDF]
Synthetic and Systems BiotechnologySynthetic RNA-based switches provide distinctive merits in modulating gene expression. Simple and flexible RNA-based switches are crucial for advancing the field of gene regulation, paving the way for innovative tools that can sense and manipulate ...
Wenhui Zhang +5 more
doaj +2 more sources
Computational De Novo Design of Group II Introns Yields Highly Active Ribozymes. [PDF]
ChembiochemGroup II Introns (G2Is) are large self‐splicing ribozymes with promising biotechnological applications. This study utilized RNA inverse folding to design three novel G2Is. The designed intron Arq.I2, an unexpectedly proficient ribozyme, self‐splices not only in vitro, but also in Escherichia coli cells.
Szokoli D +3 more
europepmc +2 more sources
Evolution of the division of labor between genes and enzymes in the RNA world. [PDF]
PLoS Computational Biology, 2014 The RNA world is a very likely interim stage of the evolution after the first replicators and before the advent of the genetic code and translated proteins.
Gergely Boza +4 more
doaj +6 more sources
From Enigma to Revelation: Unravelling Biological Functions of Ubiquitous Small Ribozymes
ChimiaRNA, widely recognized as an information-carrier molecule, is capable of catalyzing essential biological processes through ribozymes. Despite their ubiquity, specific functions in a biological context and phenotypes based on the ribozymes' activity are ...
Kasimir Kienbeck +5 more
exaly +3 more sources
Engineering of RNase P Ribozymes for Therapy against Human Cytomegalovirus Infection [PDF]
VirusesNucleic acid-based gene interference and editing strategies, such as antisense oligonucleotides, ribozymes, RNA interference (RNAi), and CRISPR/Cas9 coupled with guide RNAs, are exciting research tools and show great promise for clinical applications in ...
Adam Smith +3 more
doaj +2 more sources
A scalable system for the fast production of RNA with homogeneous terminal ends
RNA Biology, 2022 In vitro transcription (IVT) using T7 RNA polymerase has become the most common method to synthesize RNAs for use in basic research and pharmaceutical applications.
Yuchen Chen, Yan Cheng, Jinzhong Lin
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eLife, 2023 Self-cleaving ribozymes are RNA molecules that catalyze the cleavage of their own phosphodiester backbones. These ribozymes are found in all domains of life and are also a tool for biotechnical and synthetic biology applications.
Jessica M Roberts +4 more
doaj +1 more source
Single Mutation in Hammerhead Ribozyme Favors Cleavage Activity with Manganese over Magnesium
Non-Coding RNA, 2020 Hammerhead ribozymes are one of the most studied classes of ribozymes so far, from both the structural and biochemical point of views. The activity of most hammerhead ribozymes is cation-dependent. Mg2+ is one of the most abundant divalent cations in the
Mohammad Reza Naghdi +4 more
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RNA ligase ribozymes with a small catalytic core
Scientific Reports, 2023 Catalytic RNAs, or ribozymes, catalyze diverse chemical reactions that could have sustained primordial life in the hypothetical RNA world. Many natural ribozymes and laboratory evolved ribozymes exhibit efficient catalysis mediated by elaborate catalytic
Yoko Nomura, Yohei Yokobayashi
doaj +1 more source