Results 111 to 120 of about 34,904 (304)

Group I aptazymes as genetic regulatory switches

BMC Biotechnology, 2002
Background Allosteric ribozymes (aptazymes) that have extraordinary activation parameters have been generated in vitro by design and selection. For example, hammerhead and ligase ribozymes that are activated by small organic effectors and protein ...
Knudsen Scott M, Syrett Heather A, Thompson Kristin M, Ellington Andrew D   +3 more
doaj   +1 more source

Artificial and Natural Genetic Information Processing [PDF]

, 2017
Conventional methods of genetic engineering and more recent genome editing techniques focus on identifying genetic target sequences for manipulation.
Witzany, Guenther
core  

Rational Design of an Orthogonal Pair of Bimolecular RNase P Ribozymes through Heterologous Assembly of Their Modular Domains

Biology, 2019
The modular structural domains of multidomain RNA enzymes can often be dissected into separate domain RNAs and their noncovalent assembly can often reconstitute active enzymes.
Yuri Nozawa, Megumi Hagihara, Md Sohanur Rahman, Shigeyoshi Matsumura, Yoshiya Ikawa   +4 more
doaj   +1 more source

Selfishness versus functional cooperation in a stochastic protocell model [PDF]

, 2010
How to design an "evolvable" artificial system capable to increase in complexity? Although Darwin's theory of evolution by natural selection obviously offers a firm foundation, little hope of success seems to be expected from the explanatory adequacy of ...
Agresti, Beeby, Bell, Biebricher, Branciamore, Chen, Crow, Deamer, Drake, Elias Zintzaras, Ewens, Eörs Szathmáry, Fontanari, Grey, Gánti, Gánti, Haldane, Kacser, Kacser, Koch, Kondo, Kun, Könnyű, Luisi, Luisi, Luisi, Mansy, MathWorks, Mauro Santos, Maynard Smith, Mills, Moran, Niesert, Niesert, Oberholzer, Pohorille, Reader, Santos, Schaffner, Scheuring, Silvestre, Spiegelman, Stadler, Szabó, Szathmáry, Szathmáry, Szathmáry, Szathmáry, Szathmáry, Szostak, Takeuchi, Takeuchi, Traulsen, von Kiedrowski, von Kiedrowski, Whitesides, Zintzaras   +56 more
core   +2 more sources

Development of Enhanced Separation Techniques for Oligonucleotides Utilizing Mixed‐Mode Chromatography and 2D‐LC/UV/MS Analysis

Journal of Separation Science, Volume 48, Issue 8, August 2025.
ABSTRACT Oligonucleotides are traditionally analyzed using ion‐pair reversed‐phase liquid chromatography. In this project, we explored an alternative approach utilizing a reversed phase‐weak anion exchange mixed‐mode column, which combines separation based on both charge and hydrophobicity.
Jakob Haglöf, Jenny M. Nilsson, Jufang Wu Ludvigsson   +2 more
wiley   +1 more source

Ribozymes [PDF]

, 2009
Ribozimi su katalitičke molekule koje moraju biti savijene u visoko uređene tercijarne strukture, kao što to proteini rade da bi obavljali svoje funkcije. Glavni cilj je dovesti nukleofil i izrezujući fosfat u blizinu i aktivirati ih.
Zoko, Nikolina
core   +2 more sources

Q‐score as a reliability measure for protein, nucleic acid and small‐molecule atomic coordinate models derived from 3DEM maps

Acta Crystallographica Section D, Volume 81, Issue 8, Page 410-422, August 2025.
Q‐scores are calculated for atomic models derived from 3D electron microscopy maps, measure how well the model fits the map and reflect the quality of the map itself. Here, we develop a statistical model for Q‐scores applied to many maps and models in the EMDB and PDB, respectively, and show how it can be used to assess the reliability of entire models
Grigore Pintilie, Chenghua Shao, Zhe Wang, Brian P. Hudson, Justin W. Flatt, Michael F. Schmid, Kyle L. Morris, Stephen K. Burley, Wah Chiu   +8 more
wiley   +1 more source

Learning from ribozymes [PDF]

RNA, 2015
I had arranged to meet Tom Cech after the Pfizer Lecture he was giving at Harvard. This was 1986, RNA catalysis was pretty new, and I was a graduate student nearby at Brandeis interested in discussing with Tom the possibility of joining his group for a postdoc. His lecture was masterful—the audience in rapt attention. We all knew, and were taught, that
openaire   +3 more sources

Origin of Life

, 2012
The evolution of life has been a big enigma despite rapid advancements in the fields of biochemistry, astrobiology, and astrophysics in recent years.
A. Gibbons, Ashwini Kumar Lal, C. Bohler, C. Chyba, C. Huber, C.E. Manning, C.J. Salter, D. Penny, D. Warmflash, D.S. Heckman, D.S. Mckay, E.G. Roussel, F. Hoyle, F. Hoyle, F. Hoyle, G.F. Joyce, H.C. Arp, H.C. Urey, J. Li, J. Maddox, J. Postgate, J.B.S. Haldane, J.K. Fredrickson, J.V. Narlikar, J.W. Schopf, K. Kvenvolden, L.-H. Lin, L.E. Orgel, L.J. Richardson, M.T. Mondigan, N.C. Wickramsinghe, P. David, P. Ehrenfreund, P. Senapathy, R. Shapiro, R.D. Lorenz, S Arrhenius, S.J. Mojzsis, S.L. Miller, S.L. Miller, Stofan, T. Brock, T.D. Brack, W. Gilbert, W. Martin, Y.-J. Kuan, Y.-J. Ma   +46 more
core   +1 more source

An in vivo selection method to optimize trans-splicing ribozymes.

RNA: A publication of the RNA Society, 2012
Group I intron ribozymes can repair mutated mRNAs by replacing the 3'-terminal portion of the mRNA with their own 3'-exon. This trans-splicing reaction has the potential to treat genetic disorders and to selectively kill cancer cells or virus-infected ...
K. E. Olson, U. F. Müller
semanticscholar   +1 more source