Results 121 to 130 of about 34,904 (304)
Design and Preparation of a Multimeric Self-Cleaving Hammerhead Ribozyme
BioTechniques, 1997 The activity of a ribozyme can be impaired by additional sequences at the 5′ and 3′ termini of the catalytic sequence. To approach this problem, a system was designed that minimizes sequences upstream and downstream from active regions of a hammerhead ...
Juan Ruiz +3 more
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Advanced ScienceThe regulation of gene expression by light enables the versatile, spatiotemporal manipulation of biological function in bacterial and mammalian cells.
Georg Pietruschka +9 more
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Modulation of Group I Ribozyme Activity by Cationic Porphyrins
Biology, 2015 The effects of cationic porphyrins on the catalytic activities of four group I ribozymes were investigated. A cationic porphyrin possessing four pyridinium moieties (pPyP) inhibited two group IC3 ribozymes (Syn Rz and Azo Rz) and a group IC1 ribozyme ...
Shigeyoshi Matsumura +4 more
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Ribozymes, genomics and therapeutics
Chemistry & Biology, 1999 Genome-sequencing projects are proceeding at a rapid pace and determining the function of open reading frames is the next great challenge. Ribozymes with site-specific cleaving activity could aid greatly in this process. High-throughput screening methods to identify optimal target sites for ribozyme cleavage will provide tools for functional genomics ...
openaire +3 more sources
Synthesis of 2’-Modified Nucleotides
Molecules, 2000 We show in this work the development of modified building blocks for the synthesis of nuclease resistant hammerhead ribozymes. These modified monomers retain the 2’-OH which is essential for the activity of these molecules.
Adolfo Iribarren +2 more
doaj +1 more source
Identification of Hammerhead Ribozymes in All Domains of Life Reveals Novel Structural Variations
PLoS Comput. Biol., 2011 Hammerhead ribozymes are small self-cleaving RNAs that promote strand scission by internal phosphoester transfer. Comparative sequence analysis was used to identify numerous additional representatives of this ribozyme class than were previously known ...
J. Perreault +6 more
semanticscholar +1 more source
Efficient and Robust
mSphere, 2017 The notoriously low efficiency of Paramyxoviridae reverse genetics systems has posed a limiting barrier to the study of viruses in this family. Previous approaches to reverse genetics have utilized a wide variety of techniques to overcome the technical ...
Shannon M. Beaty +9 more
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Structure-based Search Reveals Hammerhead Ribozymes in the Human Microbiome*♦
Journal of Biological Chemistry, 2011 Deep sequencing of viral or bacterial nucleic acids monitors the presence and diversity of microbes in select populations and locations. Metagenomic study of mammalian viromes can help trace paths of viral transmissions within or between species.
Randi M. Jimenez, E. Delwart, A. Lupták
semanticscholar +1 more source
Ecology and Evolution in the RNA World Dynamics and Stability of Prebiotic Replicator Systems
Life, 2017 As of today, the most credible scientific paradigm pertaining to the origin of life on Earth is undoubtedly the RNA World scenario. It is built on the assumption that catalytically active replicators (most probably RNA-like macromolecules) may have been ...
András Szilágyi +5 more
doaj +1 more source
Construction of a ribozyme directed against human interleukin-6 mRNA: evaluation of its catalytic activity in vitro and in vivo [PDF]
, 1994 M. Mahieu +4 more
openalex +1 more source