Results 261 to 270 of about 34,904 (304)
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Fluorescent RNA labeling using self-alkylating ribozymes.
ACS Chemical Biology, 2014The ability to fluorescently label specific RNA sequences is of significant utility for both in vitro and live cell applications. Currently, most RNA labeling methods utilize RNA-nucleic acid or RNA-protein molecular recognition.
A. Sharma +6 more
semanticscholar +1 more source
Science, 1999
Ribozymes are RNA molecules that behave like enzymes, cleaving other RNA transcripts into pieces. They have potential therapeutic value because they can be designed to chew up viral RNAs or to repair damaged mRNA transcripts. However, getting the ribozyme to the correct part of the cell where the target RNA resides is a challenge.
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Ribozymes are RNA molecules that behave like enzymes, cleaving other RNA transcripts into pieces. They have potential therapeutic value because they can be designed to chew up viral RNAs or to repair damaged mRNA transcripts. However, getting the ribozyme to the correct part of the cell where the target RNA resides is a challenge.
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Biopolymers, 2003
AbstractThe hairpin ribozyme is a naturally occurring RNA that catalyzes sequence‐specific cleavage and ligation of RNA. It has been the subject of extensive biochemical and structural studies, perhaps the most detailed for any catalytic RNA to date. Comparison of the structures of its constituent domains free and fully assembled demonstrates that the ...
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AbstractThe hairpin ribozyme is a naturally occurring RNA that catalyzes sequence‐specific cleavage and ligation of RNA. It has been the subject of extensive biochemical and structural studies, perhaps the most detailed for any catalytic RNA to date. Comparison of the structures of its constituent domains free and fully assembled demonstrates that the ...
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Ribozyme Structures and Mechanisms
Annual Review of Biophysics and Biomolecular Structure, 2000▪ Abstract The past few years have seen exciting advances in understanding the structure and function of catalytic RNA. Crystal structures of several ribozymes have provided detailed insight into the folds of RNA molecules. Models of other biologically important RNAs have been constructed based on structural, phylogenetic, and biochemical data ...
Elizabeth A. Doherty, Jennifer A. Doudna
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Isolation of new ribozymes from a large pool of random sequences [see comment].
Science, 1993An iterative in vitro selection procedure was used to isolate a new class of catalytic RNAs (ribozymes) from a large pool of random-sequence RNA molecules.
D. Bartel, J. Szostak
semanticscholar +1 more source
Computational design and biosensor applications of small molecule-sensing allosteric ribozymes.
Biomacromolecules, 2013Here I describe accurate and time-efficient computational methods for designing small molecule-sensing allosteric ribozymes that serve as logic gates with NOT or YES Boolean logic functions.
Robert Penchovsky
semanticscholar +1 more source
ACS Synthetic Biology, 2012
Here we describe molecular implementations of integrated digital circuits, including a three-input AND logic gate, a two-input multiplexer, and 1-to-2 decoder using allosteric ribozymes. Furthermore, we demonstrate a multiplexer-decoder circuit.
Robert Penchovsky
semanticscholar +1 more source
Here we describe molecular implementations of integrated digital circuits, including a three-input AND logic gate, a two-input multiplexer, and 1-to-2 decoder using allosteric ribozymes. Furthermore, we demonstrate a multiplexer-decoder circuit.
Robert Penchovsky
semanticscholar +1 more source
Practical Ribozymes: Making ribozymes work in cells
Current Biology, 1994Ways are being found to increase the efficacy of ribozymes in cells, offering hope that they may soon begin to fulfill their promise as new reagents for inactivating specific RNAs in cells.
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Ribozymes as potential anti-HIV-1 therapeutic agents.
Science, 1990Certain RNA molecules, called ribozymes, possess enzymatic, self-cleaving activity. The cleavage reaction is catalytic and no energy source is required. Ribozymes of the "hammerhead" motif were identified in plant RNA pathogens.
N. Sarver +6 more
semanticscholar +1 more source
Thermodynamic Dissection of the Substrate−Ribozyme Interaction in the Hammerhead Ribozyme
Biochemistry, 1998The free energy of substrate binding to the hammerhead ribozyme was compared for 10 different hammerheads that differed in the length and sequence of their substrate recognition helices. These hammerheads were selected because neither ribozyme nor substrate oligonucleotide formed detectable alternate secondary structures.
Tracy K. Stage-Zimmermann +3 more
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