Results 41 to 50 of about 34,904 (304)
Molecular Therapy: Nucleic Acids, 2022 The naturally occurring structure and biological functions of RNA are correlated, which includes hammerhead ribozymes. We proposed new variants of hammerhead ribozymes targeting conserved structural motifs of segment 5 of influenza A virus (IAV) (+)RNA ...
Tomasz Czapik +3 more
doaj
Structural Insights Into Tautomeric Dynamics in Nucleic Acids and in Antiviral Nucleoside Analogs
Frontiers in Molecular Biosciences, 2022 DNA (2′-deoxyribonucleic acid) and RNA (ribonucleic acid) play diverse functional roles in biology and disease. Despite being comprised primarily of only four cognate nucleobases, nucleic acids can adopt complex three-dimensional structures, and RNA in ...
Bogdan I. Fedeles +2 more
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Error thresholds for self- and cross-specific enzymatic replication [PDF]
, 2010 The information content of a non-enzymatic self-replicator is limited by Eigen's error threshold. Presumably, enzymatic replication can maintain higher complexity, but in a competitive environment such a replicator is faced with two problems related to ...
Acevedo +56 more
core +4 more sources
Ribozyme Mediated gRNA Generation for In Vitro and In Vivo CRISPR/Cas9 Mutagenesis. [PDF]
PLoS ONE, 2016 CRISPR/Cas9 is now regularly used for targeted mutagenesis in a wide variety of systems. Here we report the use of ribozymes for the generation of gRNAs both in vitro and in zebrafish embryos.
Raymond Teck Ho Lee +2 more
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Nucleic Acids Research, 2016 Clustered regularly interspaced short palindromic repeat/Cas9 (CRISPR/Cas9) system has emerged in recent years as a highly efficient RNA-guided gene manipulation platform.
Li Xu +4 more
semanticscholar +1 more source
Multiple prebiotic metals mediate translation. [PDF]
, 2018 Today, Mg2+ is an essential cofactor with diverse structural and functional roles in life's oldest macromolecular machine, the translation system. We tested whether ancient Earth conditions (low O2, high Fe2+, and high Mn2+) can revert the ribosome to a ...
Bowman, Jessica C +8 more
core +1 more source
Hammerhead Ribozymes: True Metal or Nucleobase Catalysis? Where Is the Catalytic Power from?
Molecules, 2010 The hammerhead ribozyme was first considered as a metalloenzyme despite persistent inconsistencies between structural and functional data. In the last decade, metal ions were confirmed as catalysts in self-splicing ribozymes but displaced by nucleobases ...
Fabrice Leclerc
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The Effect of Environment on the Evolution and Proliferation of Protocells of Increasing Complexity
Life, 2022 The formation, growth, division and proliferation of protocells containing RNA strands is an important step in ensuring the viability of a mixed RNA–lipid world.
Suvam Roy, Supratim Sengupta
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A reverse transcriptase ribozyme [PDF]
eLife, 2017 A highly evolved RNA polymerase ribozyme was found to also be capable of functioning as a reverse transcriptase, an activity that has never been demonstrated before for RNA. This activity is thought to have been crucial for the transition from RNA to DNA genomes during the early history of life on Earth, when it similarly could have arisen as a ...
Biswajit Samanta, Gerald F Joyce
openaire +3 more sources
Molecules, 2010 In vitro selection with long random RNA libraries has been used as a powerful method to generate novel functional RNAs, although it often requires laborious structural analysis of isolated RNA molecules.
Yuki Fujita +3 more
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