Results 51 to 60 of about 34,904 (304)
Identification of antisense nucleic acid hybridization sites in mRNA molecules with self-quenching fluorescent reporter molecules [PDF]
, 2005 We describe a physical mRNA mapping strategy employing fluorescent self-quenching reporter molecules (SQRMs) that facilitates the identification of mRNA sequence accessible for hybridization with antisense nucleic acids in vitro and in vivo, real time ...
Do, Bao T. +11 more
core +2 more sources
Genome Biology, 2016 BackgroundCatalytic RNAs, or ribozymes, are regarded as fossils of a prebiotic RNA world that have remained in the genomes of modern organisms. The simplest ribozymes are the small self-cleaving RNAs, like the hammerhead ribozyme, which have been ...
Amelia Cervera +2 more
semanticscholar +1 more source
RNase H As Gene Modifier, Driver of Evolution and Antiviral Defense
Frontiers in Microbiology, 2017 Retroviral infections are ‘mini-symbiotic’ events supplying recipient cells with sequences for viral replication, including the reverse transcriptase (RT) and ribonuclease H (RNase H).
Karin Moelling +4 more
doaj +1 more source
Horizontal transfer between loose compartments stabilizes replication of fragmented ribozymes
, 2019 The emergence of replicases that can replicate themselves is a central issue in the origin of life. Recent experiments suggest that such replicases can be realized if an RNA polymerase ribozyme is divided into fragments short enough to be replicable by ...
Kamimura, Atsushi +3 more
core +2 more sources
RNase P Ribozymes Inhibit the Replication of Human Cytomegalovirus by Targeting Essential Viral Capsid Proteins. [PDF]
, 2015 An engineered RNase P-based ribozyme variant, which was generated using the in vitro selection procedure, was used to target the overlapping mRNA region of two proteins essential for human cytomegalovirus (HCMV) replication: capsid assembly protein (AP ...
LIU, Fenyong +9 more
core +3 more sources
Thirty-five years of research into ribozymes and nucleic acid catalysis: where do we stand today?
F1000Research, 2016 Since the discovery of the first catalytic RNA in 1981, the field of ribozyme research has developed from the discovery of catalytic RNA motifs in nature and the elucidation of their structures and catalytic mechanisms, into a field of engineering and ...
S. Müller +4 more
semanticscholar +1 more source
Ribozyme Activity of RNA Nonenzymatically Polymerized from 3′,5′-Cyclic GMP
Entropy, 2013 3′,5′-Cyclic GMP spontaneously nonenzymatically polymerizes in a base-catalyzed reaction affording G oligonucleotides. When reacted with fully or partially sequence-complementary RNA (oligo C), the abiotically generated oligo G RNA displays a typical ...
Samanta Pino +5 more
doaj +1 more source
Molecules, 2023 Kaposi’s sarcoma, an AIDS-defining illness, is caused by Kaposi’s sarcoma-associated herpesvirus (KSHV), an oncogenic virus. In this study, we engineered ribozymes derived from ribonuclease P (RNase P) catalytic RNA with targeting against the mRNA ...
Yujun Liu +3 more
doaj +1 more source
Circular RNAs with hammerhead ribozymes encoded in eukaryotic genomes: The enemy at home
RNA Biology, 2017 A new family of non-autonomous retrotransposons with self-cleaving hammerhead ribozymes, the so called retrozymes, has recently been found encoded in diverse plant genomes. These retroelements can be actively transcribed, and their RNAs accumulate in the
M. de la Peña, Amelia Cervera
semanticscholar +1 more source
Life, 2018 One of the most intriguing questions in biological science is how life originated on Earth. A large number of hypotheses have been proposed to explain it, each putting an emphasis on different events leading to functional translation and self-sustained ...
Dimiter Kunnev, Anastas Gospodinov
doaj +1 more source