Results 81 to 90 of about 34,904 (304)
High-throughput assay and engineering of self-cleaving ribozymes by sequencing
Nucleic Acids Research, 2015 Self-cleaving ribozymes are found in all domains of life and are believed to play important roles in biology. Additionally, self-cleaving ribozymes have been the subject of extensive engineering efforts for applications in synthetic biology.
Shungo Kobori +3 more
semanticscholar +1 more source
RNA BiologySelf-cleaving ribozymes are versatile tools for synthetic biologists when it comes to controlling gene expression. Up to date, 12 different classes are known, and over the past decades more and more details about their structure, cleavage mechanisms and ...
Dennis Kläge +2 more
doaj +1 more source
Rolling circle RNA synthesis catalyzed by RNA
eLife, 2022 RNA-catalyzed RNA replication is widely considered a key step in the emergence of life’s first genetic system. However, RNA replication can be impeded by the extraordinary stability of duplex RNA products, which must be dissociated for re-initiation of ...
Emil Laust Kristoffersen +3 more
doaj +1 more source
A minimal biochemical route towards de novo formation of synthetic phospholipid membranes. [PDF]
, 2019 All living cells consist of membrane compartments, which are mainly composed of phospholipids. Phospholipid synthesis is catalyzed by membrane-bound enzymes, which themselves require pre-existing membranes for function.
Bhattacharya, Ahanjit +3 more
core +2 more sources
Exploring the Potential and Advancements of Circular RNA Therapeutics
Exploration, EarlyView.Given the remarkable advantages in terms of stability, sustained expression profile, safety, wide range of druggable targets, scalable and cost‐effective manufacturing capabilities, circRNA is currently undergoing intensive investigation for various therapeutic applications such as vaccines, protein replacement, genetic disease treatment, gene therapy,
Lei Wang +6 more
wiley +1 more source
RNA Synthesis by in Vitro Selected Ribozymes for Recreating an RNA World
Life, 2015 The RNA world hypothesis states that during an early stage of life, RNA molecules functioned as genome and as the only genome-encoded catalyst. This hypothesis is supported by several lines of evidence, one of which is the in vitro selection of catalytic
Lyssa L. Martin, P. Unrau, U. F. Müller
semanticscholar +1 more source
Viruses and Evolution – Viruses First? A Personal Perspective
Frontiers in Microbiology, 2019 The discovery of exoplanets within putative habitable zones revolutionized astrobiology in recent years. It stimulated interest in the question about the origin of life and its evolution.
Karin Moelling +2 more
doaj +1 more source
In vitro Evolution and Engineering for Improved Ribozyme Polymerase Production [PDF]
, 2011 According to the "RNA World Hypothesis" the first self replicating molecule formed from the prebiotic chemicals is the ribozyme. A model compartmentalized self replicating system for ribozyme polymerases is designed and used to improve activity,
Ayaz Ali Khan +2 more
core +1 more source
Inhibition of Murine Cytomegalovirus Infection in Animals by RNase P-Associated External Guide Sequences. [PDF]
, 2017 External guide sequence (EGS) RNAs are associated with ribonuclease P (RNase P), a tRNA processing enzyme, and represent promising agents for gene-targeting applications as they can direct RNase-P-mediated cleavage of a target mRNA.
Li, Wei +9 more
core +2 more sources
Development of cytosine and adenine base editors for maize precision breeding
Journal of Integrative Plant Biology, EarlyView.A series of maize cytosine and adenine base editors was developed using various deaminase variants, enabling efficient multiplex genome editing in maize. Precise editing of the ZmACC1/2 genes generated herbicide‐tolerant maize germplasm, offering a solution for weed management in maize‐soybean intercropping systems.
Xiao Fu +8 more
wiley +1 more source